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10.3390/cells12202428
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Communication

Scanning Ion-Conductance Microscopy for Studying Mechanical Properties of Neuronal Cells during Local Delivery of Glutamate

by
Vasilii Kolmogorov
1,2,
Alexander Erofeev
1,
Alexander Vaneev
1,2,
Lyubov Gorbacheva
3,4,
Dmitry Kolesov
5,
Natalia Klyachko
2,
Yuri Korchev
6,7 and
Petr Gorelkin
1,*
1
Research Laboratory of Biophysics, National University of Science and Technology “MISIS”, Moscow 119049, Russia
2
Faculty of Chemistry, Lomonosov Moscow State University, Moscow 119991, Russia
3
Faculty of Biology, Lomonosov Moscow State University, Moscow 119991, Russia
4
Faculty of Biomedicine, Pirogov Russian National Research Medical University, Moscow 117997, Russia
5
Research Laboratory of SPM, Moscow Polytechnic University, Moscow 107023, Russia
6
Department of Medicine, Imperial College London, London SW7 2BX, UK
7
Nano Life Science Institute (WPI-NanoLSI), Kanazawa University, Kanazawa 920-1192, Japan
*
Author to whom correspondence should be addressed.
Cells 2023, 12(20), 2428; https://doi.org/10.3390/cells12202428
Submission received: 11 August 2023 / Revised: 6 October 2023 / Accepted: 8 October 2023 / Published: 11 October 2023
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Abstract

Mechanical properties of neuronal cells have a key role for growth, generation of traction forces, adhesion, migration, etc. Mechanical properties are regulated by chemical signaling, neurotransmitters, and neuronal ion exchange. Disturbance of chemical signaling is accompanied by several diseases such as ischemia, trauma, and neurodegenerative diseases. It is known that the disturbance of chemical signaling, like that caused by glutamate excitotoxicity, leads to the structural reorganization of the cytoskeleton of neuronal cells and the deviation of native mechanical properties. Thus, to investigate the mechanical properties of living neuronal cells in the presence of glutamate, it is crucial to use noncontact and low-stress methods, which are the advantages of scanning ion-conductance microscopy (SICM). Moreover, a nanopipette may be used for the local delivery of small molecules as well as for a probe. In this work, SICM was used as an advanced technique for the simultaneous local delivery of glutamate and investigation of living neuronal cell morphology and mechanical behavior caused by an excitotoxic effect of glutamate.
Keywords: scanning probe microscopy; scanning ion-conductance microscopy; cell biomechanics; nanopipette; local delivery; neuronal cells scanning probe microscopy; scanning ion-conductance microscopy; cell biomechanics; nanopipette; local delivery; neuronal cells

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MDPI and ACS Style

Kolmogorov, V.; Erofeev, A.; Vaneev, A.; Gorbacheva, L.; Kolesov, D.; Klyachko, N.; Korchev, Y.; Gorelkin, P. Scanning Ion-Conductance Microscopy for Studying Mechanical Properties of Neuronal Cells during Local Delivery of Glutamate. Cells 2023, 12, 2428. https://doi.org/10.3390/cells12202428

AMA Style

Kolmogorov V, Erofeev A, Vaneev A, Gorbacheva L, Kolesov D, Klyachko N, Korchev Y, Gorelkin P. Scanning Ion-Conductance Microscopy for Studying Mechanical Properties of Neuronal Cells during Local Delivery of Glutamate. Cells. 2023; 12(20):2428. https://doi.org/10.3390/cells12202428

Chicago/Turabian Style

Kolmogorov, Vasilii, Alexander Erofeev, Alexander Vaneev, Lyubov Gorbacheva, Dmitry Kolesov, Natalia Klyachko, Yuri Korchev, and Petr Gorelkin. 2023. "Scanning Ion-Conductance Microscopy for Studying Mechanical Properties of Neuronal Cells during Local Delivery of Glutamate" Cells 12, no. 20: 2428. https://doi.org/10.3390/cells12202428

APA Style

Kolmogorov, V., Erofeev, A., Vaneev, A., Gorbacheva, L., Kolesov, D., Klyachko, N., Korchev, Y., & Gorelkin, P. (2023). Scanning Ion-Conductance Microscopy for Studying Mechanical Properties of Neuronal Cells during Local Delivery of Glutamate. Cells, 12(20), 2428. https://doi.org/10.3390/cells12202428

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