Molecular Composition and Biological Activity of a Novel Acetonitrile–Water Extract of Lens Culinaris Medik in Murine Native Cells and Cell Lines Exposed to Different Chemotherapeutics Using Mass Spectrometry
, Gerardo Centoducati 2, Nicola Colonna 3 and Domenico Tricarico 1,*Round 1
Reviewer 1 Report
Despite of the novel developed formulation is improved in terms of the observed effects in respect to the previously published hydroalcoholic extract from the same labs, some questions remain open, for instance the authors proposed some candidates in the osteoblast and bone marrow cells protection against very potent cytotoxic compounds such as doxorubucine and staurosporine and in the renal and neuronal protections but no quantification of the individual molecules were provided. This need to be explained or at least justified in a specific paragraph in the discussion.
Mayor concern:
Abstract :
Line 34 please delete “helpful in sarcoma” hypothesis not tested in the present work
Results:
-figures 6, 7 and 8 can be combined in one figure
-high resolution figures must be provided.
Discussion :
-can be shorten focusing on the main findings and conclusions
-a paragraph reporting the limitation of their work must be provided in the discussion sections
Minor concerns:
English Editing is required
Line 665: replace extract with solvent
Line 678-735 shoul be more concise
Line 739: check for redundancy
Revised reference n° 38
Author Response
We thank the reviewer for his positive comment on our manuscript.
Mayor concern:
Abstract :
Line 34 Please delete “helpful in sarcoma” hypothesis not tested in the present work
ok
Results:
-figures 6, 7, and 8 can be combined into one figure
-high-resolution figures must be provided.
ok
Discussion :
-can be shorten focusing on the main findings and conclusions
ok
-a paragraph reporting the limitation of their work must be provided in the discussion sections
ok
Minor concerns:
English Editing is required
ok
Line 665: replace extract with solvent
ok
Line 678-735 should be more concise
ok
Line 739: Check for redundancy
ok
Revised reference n° 38
ok
Reviewer 2 Report
In figure 9a, change the plot and/or brown color because the groups are not differentiated
For figure 9b the pink groups do not differ from each other
In figure 10a use a different dither or color because it is difficult to distinguish the different groups of green color
Author Response
We thank the reviewer for her/his positive comment on our manuscript.
Comments and Suggestions for Authors
In figure 9a, change the plot and/or brown color because the groups are not differentiated
For figure 9b the pink groups do not differ from each other
In figure 10a use a different dither or color because it is difficult to distinguish the different groups of green color
Answer
As requested Figures 9ab and 10ab were revised to better differentiate between groups
