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Article

Efficient Generation of Pancreatic Progenitor Cells from Induced Pluripotent Stem Cells Derived from a Non-Invasive and Accessible Tissue Source—The Plucked Hair Follicle

by
Amatullah Fatehi
1,2,3,
Marwa Sadat
1,2,
Muneera Fayyad
3,
Jean Tang
2,4,
Duhyun Han
3,
Ian M. Rogers
1,2,4,* and
Drew Taylor
3,*
1
Department of Physiology, University of Toronto, Toronto, ON M5S 1A1, Canada
2
Lunenfeld Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, ON M5G 1X5, Canada
3
Acorn Biolabs Inc., Toronto, ON M5G 2N2, Canada
4
Department of Obstetrics and Gynaecology, University of Toronto, Toronto, ON M5S 1A1, Canada
*
Authors to whom correspondence should be addressed.
Cells 2024, 13(12), 1010; https://doi.org/10.3390/cells13121010
Submission received: 20 April 2024 / Revised: 5 June 2024 / Accepted: 6 June 2024 / Published: 10 June 2024
(This article belongs to the Collection Stem Cells in Tissue Engineering and Regeneration)
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Abstract

The advent of induced pluripotent stem cell (iPSC) technology has brought about transformative advancements in regenerative medicine, offering novel avenues for disease modeling, drug testing, and cell-based therapies. Patient-specific iPSC-based treatments hold the promise of mitigating immune rejection risks. However, the intricacies and costs of producing autologous therapies present commercial challenges. The hair follicle is a multi-germ layered versatile cell source that can be harvested at any age. It is a rich source of keratinocytes, fibroblasts, multipotent stromal cells, and the newly defined Hair Follicle-Associated Pluripotent Stem Cells (HAP). It can also be obtained non-invasively and transported via regular mail channels, making it the ideal starting material for an autologous biobank. In this study, cryopreserved hair follicle-derived iPSC lines (HF-iPS) were established through integration-free vectors, encompassing a diverse cohort. These genetically stable lines exhibited robust expression of pluripotency markers, and showcased tri-lineage differentiation potential. The HF-iPSCs effectively differentiated into double-positive cKIT+/CXCR4+ definitive endoderm cells and NKX6.1+/PDX1+ pancreatic progenitor cells, affirming their pluripotent attributes. We anticipate that the use of plucked hair follicles as an accessible, non-invasive cell source to obtain patient cells, in conjunction with the use of episomal vectors for reprogramming, will improve the future generation of clinically applicable pancreatic progenitor cells for the treatment of Type I Diabetes.
Keywords: induced pluripotent stem cells; regenerative medicine; autologous; non-invasive; keratinocytes; hair follicles; pancreatic progenitor cells induced pluripotent stem cells; regenerative medicine; autologous; non-invasive; keratinocytes; hair follicles; pancreatic progenitor cells

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MDPI and ACS Style

Fatehi, A.; Sadat, M.; Fayyad, M.; Tang, J.; Han, D.; Rogers, I.M.; Taylor, D. Efficient Generation of Pancreatic Progenitor Cells from Induced Pluripotent Stem Cells Derived from a Non-Invasive and Accessible Tissue Source—The Plucked Hair Follicle. Cells 2024, 13, 1010. https://doi.org/10.3390/cells13121010

AMA Style

Fatehi A, Sadat M, Fayyad M, Tang J, Han D, Rogers IM, Taylor D. Efficient Generation of Pancreatic Progenitor Cells from Induced Pluripotent Stem Cells Derived from a Non-Invasive and Accessible Tissue Source—The Plucked Hair Follicle. Cells. 2024; 13(12):1010. https://doi.org/10.3390/cells13121010

Chicago/Turabian Style

Fatehi, Amatullah, Marwa Sadat, Muneera Fayyad, Jean Tang, Duhyun Han, Ian M. Rogers, and Drew Taylor. 2024. "Efficient Generation of Pancreatic Progenitor Cells from Induced Pluripotent Stem Cells Derived from a Non-Invasive and Accessible Tissue Source—The Plucked Hair Follicle" Cells 13, no. 12: 1010. https://doi.org/10.3390/cells13121010

APA Style

Fatehi, A., Sadat, M., Fayyad, M., Tang, J., Han, D., Rogers, I. M., & Taylor, D. (2024). Efficient Generation of Pancreatic Progenitor Cells from Induced Pluripotent Stem Cells Derived from a Non-Invasive and Accessible Tissue Source—The Plucked Hair Follicle. Cells, 13(12), 1010. https://doi.org/10.3390/cells13121010

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