Preliminary Single-Cell RNA-Sequencing Analysis Uncovers Adipocyte Heterogeneity in Lipedema
Abstract
:1. Introduction
2. Materials and Methods
2.1. Patient Recruitment and Sample Collection
2.2. Adipose Tissue Homogenization, Enzymatic Cell Dissociation, and Single-Cell RNA Sequencing
2.3. Complementary DNA Synthesis and Amplification
2.4. Data Processing and Statistical Analysis
3. Results and Discussion
4. Summary and Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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LIPEDEMA CLASSIFICATION: STAGES (I–III) AND MORPHOLOGY (I–V) | |||||
---|---|---|---|---|---|
STAGES | Stage I Thickened but soft subcutaneous tissue with small palpable nodules; smooth skin surface | Stage II Thickened subcutaneous tissue, soft; some larger nodules; uneven skin surface. | Stage III Thickened subcutaneous tissue; indurated, large nodules and formation of folds | ||
MORPHOLOGY | Type I: Buttock | Type II: Thigh | Type III: Entire leg | Type IV: Arm (often associated withType II/III) | Type V: Lower Leg |
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Pagani, A.; Duscher, D.; Kempa, S.; Ghods, M.; Prantl, L. Preliminary Single-Cell RNA-Sequencing Analysis Uncovers Adipocyte Heterogeneity in Lipedema. Cells 2024, 13, 1028. https://doi.org/10.3390/cells13121028
Pagani A, Duscher D, Kempa S, Ghods M, Prantl L. Preliminary Single-Cell RNA-Sequencing Analysis Uncovers Adipocyte Heterogeneity in Lipedema. Cells. 2024; 13(12):1028. https://doi.org/10.3390/cells13121028
Chicago/Turabian StylePagani, Andrea, Dominik Duscher, Sally Kempa, Mojtaba Ghods, and Lukas Prantl. 2024. "Preliminary Single-Cell RNA-Sequencing Analysis Uncovers Adipocyte Heterogeneity in Lipedema" Cells 13, no. 12: 1028. https://doi.org/10.3390/cells13121028