LIP1 Regulates the Plant Circadian Oscillator by Modulating the Function of the Clock Component GIGANTEA
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials and Growth Conditions
2.2. Analysis of Gene Expression
2.3. Yeast Two-Hybrid Screening and Testing Pairwise Protein Interactions
2.4. Analysis of Pavement Cell Morphology
2.5. Physiological Assays
2.6. Statistical Analysis
3. Results
3.1. Pattern and Level of Clock Gene Expression in the lip1-2 Mutant
3.2. Identification of Proteins Interacting with LIP1
3.3. Genetic Analysis Identifies GI as the Clock Component Targeted by LIP1
3.4. Additive Effects of LIP1 and GI on Salt Stress Responses and Photomorphogenesis
3.5. Regulation of Cell Morphology and Control of Flowering Time Are Not Shared Functions of LIP1 and GI
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Marker | Genotype | Period (h) ± SE | p-Values |
---|---|---|---|
CCR2:LUC | Col | 27.71 ± 0.25 | <0.001 |
lip1-2 | 24.41 ± 0.29 | 0.009 | |
gi-101 | 25.91 ± 0.23 | 0.35 | |
lip1-2 gi-101 | 25.72 ± 0.51 | - | |
CAB2:LUC | Col | 27.36 ± 0.52 | <0.001 |
lip1-2 | 25.02 ± 0.27 | 0.016 | |
toc1-4 | 25.88 ± 0.38 | 0.006 | |
lip1-2 toc1-4 | 24.26 ± 0.41 | - | |
CCR2:LUC | Col | 27.81 ± 0.33 | 0.002 |
lip1-2 | 24.16 ± 0.71 | <0.001 | |
ztl-3 | 31.12 ± 0.44 | 0.021 | |
lip1-2 ztl-3 | 29.48 ± 0.63 | - | |
CCR2:LUC | Col | 27.43 ± 0.38 | <0.001 |
lip1-2 | 24.38 ± 0.54 | 0.027 | |
cca1-1 | 25.79 ± 0.65 | 0.007 | |
lip1-2 cca1-1 | 23.51 ± 0.18 | - |
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Hajdu, A.; Nyári, D.; Terecskei, K.; Gyula, P.; Ádám, É.; Dobos, O.; Mérai, Z.; Kozma-Bognár, L. LIP1 Regulates the Plant Circadian Oscillator by Modulating the Function of the Clock Component GIGANTEA. Cells 2024, 13, 1503. https://doi.org/10.3390/cells13171503
Hajdu A, Nyári D, Terecskei K, Gyula P, Ádám É, Dobos O, Mérai Z, Kozma-Bognár L. LIP1 Regulates the Plant Circadian Oscillator by Modulating the Function of the Clock Component GIGANTEA. Cells. 2024; 13(17):1503. https://doi.org/10.3390/cells13171503
Chicago/Turabian StyleHajdu, Anita, Dóra Nyári, Kata Terecskei, Péter Gyula, Éva Ádám, Orsolya Dobos, Zsuzsanna Mérai, and László Kozma-Bognár. 2024. "LIP1 Regulates the Plant Circadian Oscillator by Modulating the Function of the Clock Component GIGANTEA" Cells 13, no. 17: 1503. https://doi.org/10.3390/cells13171503