DNase II Can Efficiently Digest RNA and Needs to Be Redefined as a Nuclease
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Collection of Saliva
2.3. Degradation of Yeast RNA by Saliva
2.4. Degradation of Nucleic Acids by DNase II
2.5. Exonuclease Digestion of Products after RNA Digestion by DNase II
2.6. Dephosphorylation of RNA Products Digested by DNase II
2.7. Phosphorylation of Purchased DNA
2.8. Ligation of Products Digested by DNase II
3. Results
3.1. Efficient Degradation of RNA by DNase II under Acidic Conditions
3.2. RNA Is Digested by DNase II Similarly to ssDNA
3.3. Determination of Terminal Structure of RNA Cleavage Products
3.4. DNase II Only Has Weak Sequence Specificity for Cleaving RNA and Can Cleave Most RNA Sequences Efficiently under Relatively Strong Conditions
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Zhuang, J.; Du, X.; Liu, K.; Hao, J.; Wang, H.; An, R.; Liang, X. DNase II Can Efficiently Digest RNA and Needs to Be Redefined as a Nuclease. Cells 2024, 13, 1525. https://doi.org/10.3390/cells13181525
Zhuang J, Du X, Liu K, Hao J, Wang H, An R, Liang X. DNase II Can Efficiently Digest RNA and Needs to Be Redefined as a Nuclease. Cells. 2024; 13(18):1525. https://doi.org/10.3390/cells13181525
Chicago/Turabian StyleZhuang, Jingyun, Xinmei Du, Kehan Liu, Jing Hao, Haoyu Wang, Ran An, and Xingguo Liang. 2024. "DNase II Can Efficiently Digest RNA and Needs to Be Redefined as a Nuclease" Cells 13, no. 18: 1525. https://doi.org/10.3390/cells13181525