Transgene-Free Cynomolgus Monkey iPSCs Generated under Chemically Defined Conditions

Tereshchenko, Yuliia; Esiyok, Nesil; Garea-Rodríguez, Enrique; Repetto, Daniele; Behr, Rüdiger; Rodríguez-Polo, Ignacio

doi:10.3390/cells13060558

Open AccessCommunication

Transgene-Free Cynomolgus Monkey iPSCs Generated under Chemically Defined Conditions

by

Yuliia Tereshchenko

^1,2,†,

Nesil Esiyok

^1,†,

Enrique Garea-Rodríguez

³,

Daniele Repetto

³,

Rüdiger Behr

^1,2,*

and

Ignacio Rodríguez-Polo

^{1,2,4,*,†,‡}

¹

Research Platform Degenerative Diseases, German Primate Center-Leibniz Institute for Primate Research, Kellnerweg 4, 37077 Göttingen, Germany

²

German Center for Cardiovascular Research (DZHK), Partner Site Göttingen, 37075 Göttingen, Germany

³

Charles River Laboratories Germany GmbH, Hans-Adolf-Krebs-Weg 9, 37077 Göttingen, Germany

⁴

Department of Developmental Biology, Göttingen Center for Molecular Biosciences, University of Göttingen, Justus-von-Liebig Weg 11, 37077 Göttingen, Germany

^*

Authors to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

^‡

Current address: Stem Cell & Human Development Laboratory, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, UK.

Cells 2024, 13(6), 558; https://doi.org/10.3390/cells13060558

Submission received: 20 December 2023 / Revised: 11 March 2024 / Accepted: 15 March 2024 / Published: 21 March 2024

(This article belongs to the Collection Stem Cells in Tissue Engineering and Regeneration)

Download

Browse Figures

Review Reports Versions Notes

Abstract

Non-human primates (NHPs) are pivotal animal models for translating novel cell replacement therapies into clinical applications, including validating the safety and efficacy of induced pluripotent stem cell (iPSC)-derived products. Preclinical development and the testing of cell-based therapies ideally comprise xenogeneic (human stem cells into NHPs) and allogenic (NHP stem cells into NHPs) transplantation studies. For the allogeneic approach, it is necessary to generate NHP-iPSCs with generally equivalent quality to the human counterparts that will be used later on in patients. Here, we report the generation and characterization of transgene- and feeder-free cynomolgus monkey (Macaca fascicularis) iPSCs (Cyno-iPSCs). These novel cell lines have been generated according to a previously developed protocol for the generation of rhesus macaque, baboon, and human iPSC lines. Beyond their generation, we demonstrate the potential of the novel Cyno-iPSCs to differentiate into two clinically relevant cell types, i.e., cardiomyocytes and neurons. Overall, we provide a resource of novel iPSCs from the most frequently used NHP species in the regulatory testing of biologics and classical pharmaceutics to expand our panel of iPSC lines from NHP species with high relevance in preclinical testing and translational research.

Keywords: macaque; iPSC; stem cell; non-human primate; neuronal differentiation; cardiac differentiation; regeneration

Share and Cite

MDPI and ACS Style

Tereshchenko, Y.; Esiyok, N.; Garea-Rodríguez, E.; Repetto, D.; Behr, R.; Rodríguez-Polo, I. Transgene-Free Cynomolgus Monkey iPSCs Generated under Chemically Defined Conditions. Cells 2024, 13, 558. https://doi.org/10.3390/cells13060558

AMA Style

Tereshchenko Y, Esiyok N, Garea-Rodríguez E, Repetto D, Behr R, Rodríguez-Polo I. Transgene-Free Cynomolgus Monkey iPSCs Generated under Chemically Defined Conditions. Cells. 2024; 13(6):558. https://doi.org/10.3390/cells13060558

Chicago/Turabian Style

Tereshchenko, Yuliia, Nesil Esiyok, Enrique Garea-Rodríguez, Daniele Repetto, Rüdiger Behr, and Ignacio Rodríguez-Polo. 2024. "Transgene-Free Cynomolgus Monkey iPSCs Generated under Chemically Defined Conditions" Cells 13, no. 6: 558. https://doi.org/10.3390/cells13060558

APA Style

Tereshchenko, Y., Esiyok, N., Garea-Rodríguez, E., Repetto, D., Behr, R., & Rodríguez-Polo, I. (2024). Transgene-Free Cynomolgus Monkey iPSCs Generated under Chemically Defined Conditions. Cells, 13(6), 558. https://doi.org/10.3390/cells13060558

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Menu

Transgene-Free Cynomolgus Monkey iPSCs Generated under Chemically Defined Conditions

Abstract

Share and Cite

Article Metrics

Article Access Statistics

Further Information

Guidelines

MDPI Initiatives

Follow MDPI