Breast Cancer-Derived Microparticles Reduce Cancer Cell Adhesion, an Effect Augmented by Chemotherapy
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture
2.2. Extraction and Quantification of TMP
2.3. Modified Boyden Chamber Assay
2.4. Cell Spreading Assay
2.5. Cell Viability AlamarBlueTM Assay
2.6. Pillar Fabrication
2.7. CD44 Short Hairpin RNA
2.8. Western Blot
2.9. Tumor-Derived Microparticle Proteomics
2.10. Ex Vivo Pulmonary Metastasis Assay
2.11. Blood Samples from Cancer Patients
2.12. Immunostaining and Imaging
2.13. Statistical Analysis
3. Results
3.1. TMPs from Highly Metastatic or Chemotherapy-Treated Tumor Cells Induce Tumor Cell Invasion
3.2. TMPs from Highly Metastatic or Chemotherapy-Treated Tumor Cells Inhibit Tumor Cell Seeding
3.3. TMPs from Highly Metastatic or Chemotherapy-Treated Tumor Cells Inhibit Cellular Adhesion and Destroy Actin Filaments
3.4. TMPs Increase the Pace of Metastatic Cell Biomechanical Forces
3.5. Proteomic Analysis of TMPs
3.6. CD44-Expressing TMPs Decrease Cell Adhesion and Support Tumor Cell Metastatic Properties
3.7. TMPs Expressing CD44 were Increased in Breast Cancer Patients Treated with Chemotherapy
4. Discussion
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Shechter, D.; Harel, M.; Mukherjee, A.; Sagredo, L.M.; Loven, D.; Prinz, E.; Avraham, S.; Orian-Rousseau, V.; Geiger, T.; Shaked, Y.; et al. Breast Cancer-Derived Microparticles Reduce Cancer Cell Adhesion, an Effect Augmented by Chemotherapy. Cells 2020, 9, 2269. https://doi.org/10.3390/cells9102269
Shechter D, Harel M, Mukherjee A, Sagredo LM, Loven D, Prinz E, Avraham S, Orian-Rousseau V, Geiger T, Shaked Y, et al. Breast Cancer-Derived Microparticles Reduce Cancer Cell Adhesion, an Effect Augmented by Chemotherapy. Cells. 2020; 9(10):2269. https://doi.org/10.3390/cells9102269
Chicago/Turabian StyleShechter, Dvir, Michal Harel, Abhishek Mukherjee, Leonel M. Sagredo, David Loven, Elad Prinz, Shimrit Avraham, Veronique Orian-Rousseau, Tamar Geiger, Yuval Shaked, and et al. 2020. "Breast Cancer-Derived Microparticles Reduce Cancer Cell Adhesion, an Effect Augmented by Chemotherapy" Cells 9, no. 10: 2269. https://doi.org/10.3390/cells9102269