Perlecan Facilitates Neuronal Nitric Oxide Synthase Delocalization in Denervation-Induced Muscle Atrophy
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Denervation Surgery
2.3. Muscle Sampling
2.4. Immunofluorescence Microscopy
2.5. Total Protein Extraction
2.6. Western Blotting
2.7. Statistical Analyses
3. Results
3.1. Changes in Muscle Weight after Denervation Surgery
3.2. Changes in Membrane nNOS Expression
3.3. FoxO Signaling and Ubiquitin—Proteasome Signaling Activation
4. Discussion
4.1. Perlecan Promotes Skeletal Muscle Atrophy Following Denervation
4.2. Perlecan Facilitates nNOS Delocalization, FoxO Signaling, and Ubiquitin—Proteasome System Activation
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Nakada, S.; Yamashita, Y.; Machida, S.; Miyagoe-Suzuki, Y.; Arikawa-Hirasawa, E. Perlecan Facilitates Neuronal Nitric Oxide Synthase Delocalization in Denervation-Induced Muscle Atrophy. Cells 2020, 9, 2524. https://doi.org/10.3390/cells9112524
Nakada S, Yamashita Y, Machida S, Miyagoe-Suzuki Y, Arikawa-Hirasawa E. Perlecan Facilitates Neuronal Nitric Oxide Synthase Delocalization in Denervation-Induced Muscle Atrophy. Cells. 2020; 9(11):2524. https://doi.org/10.3390/cells9112524
Chicago/Turabian StyleNakada, Satoshi, Yuri Yamashita, Shuichi Machida, Yuko Miyagoe-Suzuki, and Eri Arikawa-Hirasawa. 2020. "Perlecan Facilitates Neuronal Nitric Oxide Synthase Delocalization in Denervation-Induced Muscle Atrophy" Cells 9, no. 11: 2524. https://doi.org/10.3390/cells9112524