A Clonal NG2-Glia Cell Response in a Mouse Model of Multiple Sclerosis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. StarTrack DNA Vectors
2.3. In Utero Electroporation (IUE)
2.4. EAE Induction
2.5. Immunohistochemistry
2.6. Imaging Acquisition and Data Analysis
3. Results
3.1. Spatial Distribution of the Cortical Progeny of NG2-Glia Derived from Single Embryonic Progenitors in EAE-Lesioned Mouse Brain
3.2. Clonal Distribution of the Cell Progeny from Individual Embryonic Pallial NG2-Glia Progenitors in EAE-Lesioned Brains
3.3. Relationship Between Sibling NG2-Glia and EAE Brain Lesions
3.4. Morphometric Analyses of NG2-Glia in EAE Lesions
3.5. Clonal NG2-Glia Response to EAE Lesion
4. Discussion
4.1. Heterogeneity in the Clonal Distribution of NG2-Glia in a Mouse Model of MS
4.2. Heterogenic and Clonal NG2-Glia Responses to EAE Lesions
4.3. NG2-Glia Activate in Response to EAE Lesions, Undergoing a Progressive Morphological Change to Reactivity Characterized by an Increase in Their Size, Domain, and Arborization
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Barriola, S.; Pérez-Cerdá, F.; Matute, C.; Bribián, A.; López-Mascaraque, L. A Clonal NG2-Glia Cell Response in a Mouse Model of Multiple Sclerosis. Cells 2020, 9, 1279. https://doi.org/10.3390/cells9051279
Barriola S, Pérez-Cerdá F, Matute C, Bribián A, López-Mascaraque L. A Clonal NG2-Glia Cell Response in a Mouse Model of Multiple Sclerosis. Cells. 2020; 9(5):1279. https://doi.org/10.3390/cells9051279
Chicago/Turabian StyleBarriola, Sonsoles, Fernando Pérez-Cerdá, Carlos Matute, Ana Bribián, and Laura López-Mascaraque. 2020. "A Clonal NG2-Glia Cell Response in a Mouse Model of Multiple Sclerosis" Cells 9, no. 5: 1279. https://doi.org/10.3390/cells9051279