Evidence of Interspecific Chromosomal Diversification in Rainbowfishes (Melanotaeniidae, Teleostei)
Abstract
:1. Introduction
2. Materials and Methods
2.1. Studied Material
2.2. Chromosome Preparation and Staining
2.3. Fluorescence In Situ Hybridisation (FISH) with Telomeric and rRNA Genes Probes
2.4. Microscopy and Image Analyses
3. Results
3.1. Karyotypes
3.2. rDNA Chromosome Mapping
3.3. Telomere Mapping
3.4. CMA3/DAPI Staining
3.5. C Banding
4. Discussion
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Species | No. of Individuals | No. of Metaphases Examined | ||||
---|---|---|---|---|---|---|
Giemsa | C Banding | CMA3 | Telomeres | FISH | ||
Melanotaenia sp. | 3 ; 3 | 50 | 13 | 31 | 10 | 15 |
Glossolepis incisus | 2 ; 2 | 22 | 21 | 13 | 12 | 16 |
Iriatherina werneri | 2 ; 5 | 25 | 35 | 30 | 35 | 28 |
Rhadinocentrus ornatus | 2 ; 2 | 40 | 14 | 10 | 59 | 22 |
Cairnsichthys rhombosomoides | 2 ; 2 | 80 | 40 | 15 | 14 | 28 |
Species | 2n | Karyotype Composition | NF | 28S rDNA Clusters | 5S rDNA Clusters | Telomeres | AT-Rich/GC-Rich Regions | Heterochromatin Regions |
---|---|---|---|---|---|---|---|---|
Melanotaenia sp. | 48 | 48st/a | 48 | 2 | 4 | no ITS | 2 pericenromeric | 32 centromeric |
Glossolepis incisus | 48 | 48st/a | 48 | 2 | 4 | no ITS | 2 pericenromeric | 14 centromeric |
Iriatherina werneri | 48 | 48st/a | 48 | 2 | 4 | no ITS | 2 pericenromeric | 36 centromeric 4 interstitial |
Rhadinocentrus ornatus | 48 | 12sm + 36st/a | 60 | 2 | 4 | no ITS | 2 telomeric | 28 centromeric 2telomeric |
Cairnsichthys rhombosomoides | 48 | 8sm + 40st/a | 56 | 2 | 8 | no ITS | 2 pericenromeric | 8 centromeric 4 interstitial 2 large clusters |
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Majtánová, Z.; Unmack, P.J.; Prasongmaneerut, T.; Shams, F.; Srikulnath, K.; Ráb, P.; Ezaz, T. Evidence of Interspecific Chromosomal Diversification in Rainbowfishes (Melanotaeniidae, Teleostei). Genes 2020, 11, 818. https://doi.org/10.3390/genes11070818
Majtánová Z, Unmack PJ, Prasongmaneerut T, Shams F, Srikulnath K, Ráb P, Ezaz T. Evidence of Interspecific Chromosomal Diversification in Rainbowfishes (Melanotaeniidae, Teleostei). Genes. 2020; 11(7):818. https://doi.org/10.3390/genes11070818
Chicago/Turabian StyleMajtánová, Zuzana, Peter J. Unmack, Tulyawat Prasongmaneerut, Foyez Shams, Kornsorn Srikulnath, Petr Ráb, and Tariq Ezaz. 2020. "Evidence of Interspecific Chromosomal Diversification in Rainbowfishes (Melanotaeniidae, Teleostei)" Genes 11, no. 7: 818. https://doi.org/10.3390/genes11070818
APA StyleMajtánová, Z., Unmack, P. J., Prasongmaneerut, T., Shams, F., Srikulnath, K., Ráb, P., & Ezaz, T. (2020). Evidence of Interspecific Chromosomal Diversification in Rainbowfishes (Melanotaeniidae, Teleostei). Genes, 11(7), 818. https://doi.org/10.3390/genes11070818