Study of Quasispecies Complexity and Liver Damage Progression after Liver Transplantation in Hepatitis C Virus Infected Patients
Abstract
:1. Introduction
2. Materials and Methods
2.1. RNA Extraction, RT-PCR, Heminested-PCR Amplification and Quantification
2.2. Ultradeep Pyrosequencing (UDPS)
2.3. Nucleotide Haplotypes and Diversity Analyses
2.4. Phylogenetic Analysis
2.5. Biostatistic Analyses
3. Results
3.1. Viral Kinetics and Histological Grade
3.2. Phylogenetic Studies
3.3. Unidimensional Analyses
3.4. Principal Components Analysis
3.5. Associations between Diversity Measures and Fibrosis at 1 Year Post-LT
3.6. Associations between VL and Fibrosis at 1 Year Post-LT
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Diversity Measures | ||||||
---|---|---|---|---|---|---|
Adjusted R2 (D0) | Adjusted R2 (D1) | Adjusted R2 (D2) | Adjusted R2 (Dinf) | Adjusted R2 (Mf) | Adjusted R2 (π) | |
Pre-LT | 0.086 | 0.064 | 0.118 | 0.111 | 0.021 | 0.027 |
Post-LT | 0.060 | 0.026 | 0.019 | 0.025 | 0.063 | 0.051 |
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Llorens-Revull, M.; Gregori, J.; Dopazo, C.; Rodriguez-Frías, F.; Garcia-Cehic, D.; Soria, M.E.; Chen, Q.; Rando, A.; Perales, C.; Esteban, J.I.; et al. Study of Quasispecies Complexity and Liver Damage Progression after Liver Transplantation in Hepatitis C Virus Infected Patients. Genes 2021, 12, 1731. https://doi.org/10.3390/genes12111731
Llorens-Revull M, Gregori J, Dopazo C, Rodriguez-Frías F, Garcia-Cehic D, Soria ME, Chen Q, Rando A, Perales C, Esteban JI, et al. Study of Quasispecies Complexity and Liver Damage Progression after Liver Transplantation in Hepatitis C Virus Infected Patients. Genes. 2021; 12(11):1731. https://doi.org/10.3390/genes12111731
Chicago/Turabian StyleLlorens-Revull, Meritxell, Josep Gregori, Cristina Dopazo, Francisco Rodriguez-Frías, Damir Garcia-Cehic, Maria Eugenia Soria, Qian Chen, Ariadna Rando, Celia Perales, Juan Ignacio Esteban, and et al. 2021. "Study of Quasispecies Complexity and Liver Damage Progression after Liver Transplantation in Hepatitis C Virus Infected Patients" Genes 12, no. 11: 1731. https://doi.org/10.3390/genes12111731