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Article

Role of Calcium/Calcineurin Signalling in Regulating Intracellular Reactive Oxygen Species Homeostasis in Saccharomyces cerevisiae

by
Guohui Li
1,2,
Wenxuan Fu
3,
Yu Deng
1,2 and
Yunying Zhao
1,3,*
1
National Engineering Laboratory for Cereal Fermentation Technology (NELCF), School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China
2
Jiangsu Provincial Research Center for Bioactive Product Processing Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China
3
Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China
*
Author to whom correspondence should be addressed.
Genes 2021, 12(9), 1311; https://doi.org/10.3390/genes12091311
Submission received: 29 June 2021 / Revised: 19 August 2021 / Accepted: 22 August 2021 / Published: 25 August 2021
(This article belongs to the Special Issue Yeast Applications in Gene Mutation)
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Abstract

:
The calcium/calcineurin signalling pathway is required for cell survival under various environmental stresses. Using Saccharomyces cerevisiae, we explored the mechanism underlying calcium-regulated homeostasis of intracellular reactive oxygen species (ROS). We found that deletion of acyltransferase Akr1 and C-5 sterol desaturase Erg3 increased the intracellular ROS levels and cell death, and this could be inhibited by the addition of calcium. The hexose transporter Hxt1 and the amino acid permease Agp1 play crucial roles in maintaining intracellular ROS levels, and calcium induced the expression of the HXT1 and AGP1 genes. The cytosolic calcium concentration was decreased in both the akr1Δ and erg3Δ mutants relative to wild-type cells, potentially lowering basal expression of HXT1 and AGP1. Moreover, the calcium/calcineurin signalling pathway also induced the expression of AKR1 and ERG3, indicating that Akr1 and Erg3 might perform functions that help yeast cells to survive under high calcium concentrations. Our results provided mechanistic insight into how calcium regulated intracellular ROS levels in yeast.

Graphical Abstract

1. Introduction

Calcium ions are intracellular signalling molecules that regulate many cellular processes in eukaryotic cells including programmed cell death, muscle contraction and cell proliferation [1]. The concentration of Ca2+ in organelles can be maintained in an optimal range via the calcium signalling pathway. For example, in Saccharomyces cerevisiae the cytoplasmic Ca2+ concentration is kept in the optimal range of 50–200 nM when it is lower than 1 μΜ or higher than 100 mM in the external environment [2,3]. Moreover, the calcium homeostasis system and signalling regulation pathway in yeast cells are conserved, and similar to those in mammalian cardiac muscle cells [4,5]. Therefore, the budding yeast S. cerevisiae provides powerful genetic and genomic tools for exploring calcium homeostasis and the signal transduction system in yeast cells, and the knowledge may be applicable to higher eukaryotes and useful for curing human diseases, such as pathological heart enlargement and heart failure [6,7].
Reactive oxygen species (ROS) play important roles in the regulation of cellular processes such as apoptosis and oxidative stress. Accumulated ROS inside cells cause oxidative damage to proteins, nucleic acids and other biological macromolecules [8]. Moreover, the accumulation of ROS plays important roles in cell death and necrosis in a variety of types of cells [9]. Importantly, oxidative damage and programmed cell death caused by ROS have been associated with serious human diseases such as heart disease, amyotrophic lateral sclerosis and other serious ailments [10]. In addition, ROS play crucial roles in cell aging and cancer development [11]. In aerobic respiration, electrons leak from the electron transport and generate ROS. Additionally, exposure to heavy metals, herbicides, ultraviolet radiation, chemicals, air pollution and other exogenous factors can also lead to ROS generation [12]. Many factors affect the transition from respiration to aerobic fermentation, including glucose transport, glycolytic enzymes and transcriptional factors that regulate the expression of genes involved in respiratory depression. In S. cerevisiae, glucose itself transmits respiratory inhibition signals through the Crabtree effect [13]. Glucose activates a series of signal transduction pathways involving G-protein-coupled receptors, hexokinases and transmembrane glucose receptors that work together to inhibit respiration and promote aerobic fermentation [14].
Mitochondria are the major site of energy metabolism in eukaryotic cells, and ATP is synthesised in these organelles through oxidative phosphorylation (ox-phos) [15]. Mitochondria are also the site of porphyrin and steroid hormones and lipid metabolism [16], and they play crucial roles in regulating intracellular Ca2+ homeostasis [17] and glucose sensing [18]. The main source of ROS is mitochondrial respiration, in which single electron reduction produces O2, superoxide anions and their derivatives [15]. Ca2+ is a ubiquitous signalling molecule in eukaryotic cells, and many pathological diseases are caused by Ca2+ imbalance in mitochondria [19]. Ca2+ overload in the mitochondrial matrix leads to the production of excessive ROS, the permeability transition pore of mitochondria is opened, and cytochrome C is released, which eventually leads to cell death [20,21]. When yeast cells are only stimulated by Ca2+, the increase in Ca2+ concentration in mitochondria promotes the production of more ATP in mitochondria by increasing respiratory chain activity, thus stimulating cell growth [15]. However, when the concentration of calcium ions exceeds a certain range, ROS levels in cells can increase, as occurs following stimulation by external pathogenic drugs, and in severe cases this can lead to cell death [22,23].
The absence of the ERG3 gene, encoding a C-5 sterol desaturase, leads to the failure of sterol synthesis, resulting in a change in lipid composition in the cell membrane [24,25]. Furthermore, the permeability of the cell membrane is altered, more ROS enter the cell, and intracellular ROS levels increase [15]. AKR1 encodes an integral membrane protein that is responsible for the palmitoylation of some proteins [26]. The HIP14/AKRL1 protein acyltransferase in mammals is a homolog of Akr1 that can modify several neuronal proteins [27,28]. In budding yeast, deletion of the AKR1 gene prevents palmitoylation of Yck1 and Yck2, preventing them from being correctly located in the cytoplasmic membrane, resulting in increased intracellular respiration, production of excessive ROS, and ultimately cell death [29].
In our previous study, we identified mutants of ERG3 and AKR1 that were sensitive to high calcium stress [30]. Herein, we describe a new role for calcium in regulating intracellular ROS levels in akr1∆ and erg3∆ mutants. Deletion of ERG3 or AKR1 elevated intracellular ROS levels in yeast cells and calcium could inhibit this increase. The mechanism did not involve calcium-regulated restoration of membrane localisation of Yck1 and Yck2 but involved calcium-induced expression of HXT1 and AGP1. In addition, calcium also induced the expression of AKR1 and ERG3, and cytosolic calcium levels in akr1∆ and erg3∆ mutants were lower than in wild-type (WT) cells. We therefore speculate that, in WT cells, calcium induces AKR1 and ERG3 expression to protect cells against high calcium stress. When AKR1 or ERG3 is deleted, the cytosolic calcium concentration is reduced, which leads to decreased expression of HXT1 and AGP1. Adding calcium upregulated HXT1 and AGP1 expression to reduce intracellular ROS levels to normal ranges.

2. Materials and Methods

2.1. Yeast Strains and Growth Media

The S. cerevisiae strains used in this study are listed in Table 1. YPD medium (2% w/v glucose, 2% w/v tryptone and 1% w/v yeast extract) was used to culture yeast cells under nonselective conditions. SC-URA or SC-HIS medium (0.17% w/v yeast nitrogen base, 2% w/v glucose, 0.5% ammonia sulphate) lacking uracil or histidine was used for maintenance of plasmids or selection, respectively. Solid media were prepared by adding 2% (w/v) agar to YPD or SC media when necessary. All yeast strains were cultured at 30 °C.

2.2. DNA Manipulations

To investigate the localisation of Yck1 and Yck2, the fusion proteins of GFP-Yck1, GFP-Yck2 were constructed in the WT BY4741, akr1Δ, and erg3Δ mutant strains, respectively. To achieve this, the HIS3MX6-PADH1-GFP cassette was first amplified with primers YCK1-NGFP-F/YCK1-NGFP-R, YCK2-NGFP-F/YCK2-NGFP-R from plasmid pEASY-T1-HIS3MX6-PADH1-GFP [32], respectively, and integrated into the WT BY4741, akr1Δ, or erg3Δ mutant strains. Correct integration was confirmed by PCR with primers GFP-check-F and YCK1-check-R or YCK2-check-R, respectively. To construct the Akr1-GFP and Erg3-GFP fusion proteins in the WT BY4741, the GFP-kanMX6 cassette was amplified with primers AKR1-CGFP-F/AKR1-CGFP-R or ERG3-CGFP-F/ERG3-CGFP-R from the plasmid pFA6a-GFP-kanMX6 [33], respectively. To overexpress HXT1 or AGP1 gene in akr1Δ and erg3Δ mutant strains, the HIS3MX6-PADH1 cassette was first amplified with primers HXT1-NF/HXT1-NR or AGP1-NF/AGP1-NR from HIS3MX6-PADH1-GFP plasmid and then integrated into akr1Δ and erg3Δ mutants. The correct transformants were tested by PCR with primers HXT1-check-F/HXT1-check-R or AGP1-check-F/AGP1-check-R, respectively. All the transformants were screened in solid SD-HIS media. The correct integration was confirmed by PCR with primers AKR1-check-F/AKR1-check-R and ERG3-check-F ERG3-check-R, respectively. The primers were all listed in Table S1. Log phase cells expressing the indicated fusion proteins were first treated with or without 0.2 M CaCl2 for 60 min. All cells were visualised using a Nikon Eclipse 80i epifluorescence microscope.
To construct the HXT1-lacZ and AGP1-lacZ reporters, we first amplified the promoter of HXT1 and AGP1 with primer pairs HXT1-LF/HXT1-LR or AGP1-LF/AGP1-LR, respectively, and replaced it with the PMR1 promoter in pRS316-PMR1-lacZ [34], yielding pRS316-HXT1-lacZ and pRS316-AGP1-lacZ.

2.3. ROS and Cell Death Assay

Yeast cells were first cultured overnight in YPD medium, then inoculated into fresh YPD medium to an initial OD600 of ~0.1. When cells had grown to the middle log phase, they were split into two aliquots, and cultured in the absence or presence of 0.2 M CaCl2 for an additional 2 h. Intracellular ROS levels and cell death were measured as previously described [35,36,37]. More than 500 cells were used each analysis, and data are presented as the mean ± standard deviation (SD) from three independent samples.

2.4. The β-Galactosidase Activity Assay

To measure PHXT1- and PAGP1-driven β-galactosidase activities, pRS316-HXT1-lacZ and pRS316-AGP1-lacZ plasmids were transformed into WT BY4741, akr1Δ, erg3Δ and sod1Δ mutant strains and were screened in solid SD-URA media. Transformants were first cultured overnight in SD-URA medium, inoculated into two aliquots of fresh YPD medium and grown to the middle log phase, then treated with or without 0.2 M CaCl2 for an additional 2 h. Yeast cells were harvested by centrifugation to extract total proteins, and β-galactosidase activity was measured as described previously [34]. Data are presented as the mean ± SD from six independent experiments.

2.5. RNA Extraction and qRT-PCR Analysis

For RNA preparation, the indicated yeast cells were first treated with or without 0.2 M CaCl2 for 2 h. Cells were then collected and total RNA was extracted by the hot phenol method [38]. First-strand cDNA was synthesised using a Primer Script RT Reagent Kit (CWBiotech, Beijing, China) according to the manufacturer’s instructions. The mRNA expression levels of HXT1, AGP1, AKR1 and ERG3 were estimated by PCR with the primer pairs listed in Table S1. The ACT1 gene was used as an internal control. The results were analyzed through the −ΔΔCt method [39]. Each reaction was carried out in triplicate.

2.6. Cytosolic Calcium Concentration Measurements

To test the cytosolic calcium concentration, WT BY4741, akr1Δ and erg3Δ mutants were transformed with pEVP11-AEQ89 plasmid harbouring the aequorin protein [40]. Aequorin luminescence measurements were performed as described previously [32]. Luminescence was recorded every second by a Synergy H4 fluorescence reader (BioTek, Kocherwaldstr. 34, D-74177 Bad Friedrichshall, Germany) for 10 s before and 120 s after CaCl2 injection. Cytosolic free Ca2+ concentrations were calculated as described previously [41].

3. Results

3.1. Calcium Inhibits ROS and Cell Death in akr1Δ and erg3Δ Mutants

From a genome-wide screen, we previously identified 120 calcium-sensitive mutants [30]. To investigate how calcium regulates intracellular ROS levels, we investigated intracellular ROS levels in calcium-sensitive mutants. We found that cells lacking the C-5 sterol desaturase Erg3 and the integral membrane protein Akr1, which is responsible for palmitoylation of certain target proteins, exhibited elevated intracellular ROS levels compared with WT cells (Figure 1A). Interestingly, when calcium was added to the culture medium, the high intracellular ROS levels in erg3∆ and akr1∆ mutants could be inhibited (Figure 1A). Because intracellular ROS levels are closely related to cell death, we wondered whether mutants of AKR1 and ERG3 genes could also lead to greater cell death. We therefore assessed cell death in erg3∆ and akr1∆ mutants with or without calcium treatment. As shown in Figure 1B, cell death rates were higher in both erg3∆ and akr1∆ mutants than in WT cells, and calcium could also inhibit the elevated cell death in erg3∆ and akr1∆ mutants.

3.2. Akr1 and Erg3 Do Not Influence the Localisation of Yck1 and Yck2

Yck1 and Yck2 are plasma membrane-associated casein kinase 1 (CK1) isoforms in yeast. Before Yck1 and Yck2 traffic to the plasma membrane, they must first be palmitoylated by Akr1 in the Golgi [42]. Yck1 and Yck2 are essential for inhibiting cellular respiration, and the deletion of these two genes can enhance respiration, causing cells to produce excessive oxygen free radicals, leading to cell death [43]. In akr1∆ mutant cells, Yck1 and Yck2 cannot be palmitoylated and are therefore retained in the cytoplasm [44]. Here, we showed that calcium could inhibit elevated ROS levels and cell death in erg3∆ and akr1∆ mutants; hence, we evaluated whether the cytosolic localisation of Yck1 and Yck2 could be repaired by adding calcium. To achieve this, we tagged Yck1 and Yck2 with GFP at their N-termini in WT BY4741, akr1Δ and erg3Δ mutant strains to investigate their subcellular localisation. In WT BY4741 and erg3Δ mutant cells, Yck1-GFP and Yck1-GFP fusion proteins displayed a plasma membrane location with or without 0.2 M CaCl2 (Figure 2). In contrast, in akr1Δ mutant cells, Yck1-GFP and Yck1-GFP fusion proteins displayed a cytosolic localisation, and addition of calcium could not relocate these proteins to the plasma membrane. These results indicated that the inhibition of ROS generation and cell death in akr1Δ and erg3Δ mutant strains by calcium was not achieved by changing the cellular localisation of Yck1 and Yck2.

3.3. Calcium Induces the Expression of HXT1 and AGP1

The protein encoded by HXT1 is a glucose-induced hexose transporter [45], and its expression is tightly regulated by the concentration of glucose in the culture medium [46]. AGP1 is a gene encoding a low-affinity amino acid permease that is important for the uptake of amino acids as a nitrogen source in yeast cells [47,48]. The inhibition of the expression of these two genes leads to increased ROS levels in cells, leading to cell death. It has been reported that extracellular amino acids induced AGP1 expression through the casein kinase isoforms Yck1 and Yck2 [49]. Moreover, the deletion of AKR1 and superoxide dismutase 1 (SOD1) genes leads to decreased expression of HXT1 and AGP1 [50].
In order to investigate whether the inhibition of intracellular ROS levels and cell death in erg3∆ and akr1∆ mutants by calcium were related to the expression of HXT1 and AGP1 genes, we measured the expression levels of HXT1 and AGP1 in WT BY4741, akr1Δ, erg3Δ and sod1Δ mutant strains. First, we constructed the HXT1-lacZ and AGP1-lacZ reporter plasmids and measured their expression levels through β-galactosidase activity assays. As shown in Figure 3A,B, the expression of both HXT1-lacZ and AGP1-lacZ was significantly reduced in akr1Δ, erg3Δ and sod1Δ mutants compared with WT cells in YPD medium. When yeast cells were treated with 0.2 M CaCl2, the β-galactosidase activities of HXT1-lacZ and AGP1-lacZ reporters were stimulated in WT BY4741, akr1Δ, erg3Δ and sod1Δ mutant strains. Specifically, the expression level of HXT1-lacZ in akr1Δ and erg3Δ mutants were both higher than that of the WT BY4741 cells after calcium treatment. Although the expression level of AGP1-lacZ was slightly lower in akr1Δ and erg3Δ mutants than that of the WT BY4741 cells after induction by calcium (0.85- and 0.8-fold of WT, respectively), this difference is relatively smaller than when there was no calcium treatment (both 0.59 fold of WT in akr1Δ and erg3Δ mutants).
To verify the induction of HXT1 and AGP1 by calcium, we measured the relative expression levels of HXT1 and AGP1 genes by the qRT-PCR method. Expression levels of HXT1 and AGP1 were significantly up-regulated after treatment with 0.2 M CaCl2 in WT BY4741, akr1Δ and erg3Δ mutant cells (Figure 3C,D), consistent with the results of expression of the AGP1-lacZ and HXT1-lacZ reporters. These results indicate that elevated intracellular ROS levels and cell death in akr1Δ and erg3Δ mutant cells might be caused by decreased expression of HXT1 and AGP1, and calcium could inhibit these phenomena by inducing the expression of HXT1 and AGP1.
To investigate whether the high ROS levels are due to the low HXT1 or AGP1 expression, HXT1 or AGP1 were overexpressed in akr1Δ and erg3Δ mutants, respectively. As shown in Figure 4, the overexpression of both HXT1 and AGP1 could significantly reduce the elevated intracellular ROS levels in akr1Δ and erg3Δ mutants, although the ROS levels were still higher than that of WT cells. These results indicated that the expression levels of both HXT1 and AGP1 were closely related to the intracellular ROS levels of akr1Δ and erg3Δ mutants.

3.4. Calcium Positively Regulates AKR1 and ERG3 Expression

Since akr1Δ and erg3Δ mutants are sensitive to calcium [30], and because calcium can inhibit elevated intracellular ROS levels and cell death caused by the deletion of AKR1 and ERG3, we wondered why akr1Δ and erg3Δ mutants are sensitive to calcium. One reason might be that deletion of AKR1 or ERG3 can cause increased cytosolic calcium levels that lead to akr1Δ and erg3Δ mutants being sensitive to calcium responses. The other reason might be that calcium can activate AKR1 and ERG3 to perform functions that help mutant cells to survive in response to high concentrations of calcium. To test these hypotheses, we first investigated cytosolic calcium levels in WT BY4741, akr1Δ and erg3Δ mutant strains using the pEVP11-AEQ89 plasmid [40]. As shown in Figure 5, the cytosolic calcium concentrations in both akr1Δ and erg3Δ mutants were lower than in the WT BY4741 strain. This result might explain why expression of HXT1 and AGP1 was lower than in WT BY4741 without calcium treatment.
Because the reduced cytosolic calcium concentrations in akr1Δ and erg3Δ mutants are not the cause of calcium sensitivity, we next tested whether expression of AKR1 and ERG3 could be induced by calcium. The expression levels of ERG3 and AKR1 genes in BY4741 and crz1Δ strains were not significantly different without calcium treatment. Expression levels of ERG3 and AKR1 genes were significantly induced by calcium, but there was no significant difference in expression of ERG3 and AKR1 genes in crz1Δ after calcium treatment (Figure 6A,B).
We also analysed the subcellular localisation of Akr1-GFP and Egr3-GFP in WT BY4741 cells with or without calcium treatment. As shown in Figure 6C, expression of Akr1-GFP and Egr3-GFP was very low and their fluorescent location signals were very weak. After adding 0.2 M CaCl2, the Akr1-GFP fusion protein could be clearly observed in the Golgi apparatus, while the Erg3-GFP fusion protein was localised in the endoplasmic reticulum as previously reported [51]. These results indicated that calcium regulated the expression of ERG3 and AKR1 genes through the transcriptional factor Crz1.

4. Discussion

In this study, we found that mutants of C-5 sterol desaturase Erg3 and palmitoyl transferase Akr1 involved in protein palmitoylation could cause elevated intracellular ROS levels and cell death. Furthermore, the addition of calcium reversed these phenomena in the akr1Δ and erg3Δ mutant strains. The deletion of ERG3 and AKR1 led to reduced cytosolic calcium content and a consequent decrease in expression of the hexose transporter HXT1 and the amino acid permease AGP1. The addition of calcium induced expression of HXT1 and AGP1 to levels observed in WT cells, which helped to protect akr1Δ and erg3Δ mutant cells against elevated intracellular ROS and cell death. In addition, calcium induced the expression of ERG3 and AKR1 through the transcriptional factor Crz1, implying that Akr1 and Erg3 performed functions that helped yeast cells to survive in response to high concentrations of calcium.
Expression of the yeast low-affinity glucose transporter Hxt1 is regulated by glucose availability: it is activated when there is glucose in the culture medium and inhibited when glucose is scare [52]. In budding yeast, three signalling pathways (Rgt2/Snf3, AMPK and cAMP-PKA) are employed to sense glucose in the culture medium. When glucose was added to glucose-starved or glucose-derepressed cells, it has been reported that Gpr1/Gpa2 and Ras complex which can activate cAMP are needed for this process [53]. In the absence of glucose, the three pathways are inactivated by the Rgt1 repressor, which represses HXT1 expression by recruiting the corepressor complex Ssn6-Tup1 and the corepressor Mth1 to the HXT promoters [54,55,56]. Therefore, multiple signalling pathways have been reported to regulate HXT1 expression. First, the TOR kinase pathway and the PP2A protein phosphatase complex might be involved in glucose-induced HXT1 expression [57,58]. Secondly, PKA plays important roles in maintaining the stability of Hxt1 by regulating its turnover in response to glucose starvation [46]. In addition to transcriptional regulation, Hxt1 and Agp1 can also be regulated post-translationally. For instance, Hxt1 and Agp1 can be internalised and degraded in the vacuole [59,60]. Grr1, one of the F-box components of the SKp1/cullin/F-box (SCF, one class of E3 enzymes) protein is involved in induction of HXT1 and AGP1 expression by interacting with Rgt1 in response to glucose and exogenous amino acids, respectively [61,62]. Herein, we demonstrated that calcium signalling was also involved in the expression of HXT1 and AGP1, indicating that calcium signalling was also involved in the regulation of glucose-related respiration.
In yeast cells, an important function of the calcium/calcineurin-dependent signalling pathway is to regulate the expression of genes by activating the transcription factors Crz1/TCN1/Hal8 [63,64,65]. The zinc finger motif of the activated Crz1 then specifically binds to the calcineurin-dependent response element (CDRE; conserved sequence 5′-GNGGC(G/T)CA-3′) in the promoter, a 24 bp DNA sequence that is essential for the calcium/calcineurin-dependent induction of gene expression [63]. Calcium/calcineurin and Crz1 regulate various genes, including those encoding membrane-binding proteins, plasma membrane proteins and protein components of the cell wall [66]. Genes encoding proteins participating in vesicle transport, lipid/sterol synthesis and protein degradation, cell wall integrity and ion and small molecule transport are also regulated by the calcium/calcineurin signalling pathway. The calcium/calcineurin pathway also induces the expression of genes involved in key components in this and other signalling pathways, which provides various feedback mechanisms regulating this pathway and crosstalk with other signalling pathways [66]. In the present study, we demonstrated that calcium regulates the expression of HXT1, AGP1, AKR1 and ERG3. Analysis of the promoter sequences of these four genes revealed that all contain predicted CDREs in their promoter regions. Therefore, we concluded that calcium-regulated expression of these four genes mainly acted through the transcription factor Crz1 that binds CDRE motifs in their promoters. In addition, since mitochondrial respiration is a main source of ROS [15], and since mitochondria also play crucial roles in regulating intracellular calcium homeostasis [17] and glucose sensing [18], thus, the link between calcium and ROS homeostasis as well as mitochondrial respiration needs to be further studied.

5. Conclusions

In conclusion, our results unveiled a new role for calcium/calcineurin signalling in regulating intracellular ROS homeostasis. By inducing the expression of HXT1 and AGP1, calcium inhibited the elevated intracellular ROS levels and cell death in akr1∆ and erg3∆ mutants. Our studies will help elucidate the mechanisms by which the calcium/calcineurin pathway regulates intracellular ROS levels and cell death in budding yeast.

Supplementary Materials

The following are available online at https://www.mdpi.com/article/10.3390/genes12091311/s1, Table S1: Primers used in this study.

Author Contributions

Conceptualization, Y.Z. and G.L.; methodology, Y.Z.; software, W.F.; formal analysis, Y.Z. and Y.D.; data curation, G.L., Y.Z. and W.F.; writing—original draft preparation, Y.Z.; writing—review and editing, Y.D.; funding acquisition, Y.Z. and Y.D. All authors have read and agreed to the published version of the manuscript.

Funding

This work was funded by grants from the National Key R&D Program of China (2021YFC2100700 and 2019YFA0905502), the National Natural Science Foundation of China (22008088 and 21877053), the Natural Science Foundation of Jiangsu Province (BK20181345), the Open Foundation of Jiangsu Key Laboratory of Industrial Biotechnology (KLIB-KF201807), and the Top-notch Academic Programs Project of Jiangsu Higher Education Institutions.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

The data used to support the findings of this study are included within the article and the Supplementary Materials.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Wu, H.; Peisley, A.; Graef, I.A.; Crabtree, G.R. NFAT signaling and the invention of vertebrates. Trends Cell Biol. 2007, 17, 251–260. [Google Scholar] [CrossRef]
  2. Bonilla, M.; Cunningham, K.W. Calcium release and influx in yeast: TRPC and VGCC rule another kingdom. Sci. STKE 2002, 2002, pe17. [Google Scholar] [CrossRef] [PubMed]
  3. Muller, E.M.; Mackin, N.A.; Erdman, S.E.; Cunningham, K.W. Fig1p facilitates Ca2+ influx and cell fusion during mating of Saccharomyces cerevisiae. J. Biol. Chem. 2003, 278, 38461–38469. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  4. Berridge, M.J.; Bootman, M.D.; Roderick, H.L. Calcium signalling: Dynamics, homeostasis and remodelling. Nat. Rev. Mol. Cell Biol. 2003, 4, 517–529. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  5. Petersen, O.H.; Michalak, M.; Verkhratsky, A. Calcium signalling: Past, present and future. Cell Calcium 2005, 38, 161–169. [Google Scholar] [CrossRef] [PubMed]
  6. Cui, J.; Kaandorp, J.A.; Sloot, P.M.; Lloyd, C.M.; Filatov, M.V. Calcium homeostasis and signaling in yeast cells and cardiac myocytes. FEMS Yeast Res. 2009, 9, 1137–1147. [Google Scholar] [CrossRef] [Green Version]
  7. Kho, C.; Lee, A.; Jeong, D.; Oh, J.G.; Chaanine, A.H.; Kizana, E.; Park, W.J.; Hajjar, R.J. SUMO1-dependent modulation of SERCA2a in heart failure. Nature 2011, 477, 601–605. [Google Scholar] [CrossRef] [PubMed]
  8. Nita, M.; Grzybowski, A. The Role of the reactive oxygen species and oxidative stress in the pathomechanism of the age-related ocular diseases and other pathologies of the anterior and posterior eye segments in adults. Oxid. Med. Cell. Longev. 2016, 2016, 3164734. [Google Scholar] [CrossRef] [Green Version]
  9. Dixon, S.J.; Stockwell, B.R. The role of iron and reactive oxygen species in cell death. Nat. Chem. Biol. 2014, 10, 9–17. [Google Scholar] [CrossRef]
  10. Pedroso, N.; Matias, A.C.; Cyrne, L.; Antunes, F.; Borges, C.; Malho, R.; de Almeida, R.F.; Herrero, E.; Marinho, H.S. Modulation of plasma membrane lipid profile and microdomains by H2O2 in Saccharomyces cerevisiae. Free Radic. Biol. Med. 2009, 46, 289–298. [Google Scholar] [CrossRef]
  11. Pelicano, H.; Carney, D.; Huang, P. ROS stress in cancer cells and therapeutic implications. Drug Resist. Updates 2004, 7, 97–110. [Google Scholar] [CrossRef] [PubMed]
  12. Sudbrak, R.; Brown, J.; Dobson-Stone, C.; Carter, S.; Ramser, J.; White, J.; Healy, E.; Dissanayake, M.; Larregue, M.; Perrussel, M.; et al. Hailey-Hailey disease is caused by mutations in ATP2C1 encoding a novel Ca2+ pump. Hum. Mol. Genet. 2000, 9, 1131–1140. [Google Scholar] [CrossRef] [PubMed]
  13. Crabtree, H.G. Observations on the carbohydrate metabolism of tumours. Biochem. J. 1929, 23, 536–545. [Google Scholar] [CrossRef]
  14. Zaman, S.; Lippman, S.I.; Zhao, X.; Broach, J.R. How Saccharomyces responds to nutrients. Annu. Rev. Genet. 2008, 42, 27–81. [Google Scholar] [CrossRef]
  15. Brookes, P.S.; Yoon, Y.; Robotham, J.L.; Anders, M.W.; Sheu, S.S. Calcium, ATP, and ROS: A mitochondrial love-hate triangle. Am. J. Physiol. Cell Physiol. 2004, 287, C817–C833. [Google Scholar] [CrossRef]
  16. O’Reilly, C.M.; Fogarty, K.E.; Drummond, R.M.; Tuft, R.A.; Walsh, J.V., Jr. Quantitative analysis of spontaneous mitochondrial depolarizations. Biophys. J. 2003, 85, 3350–3357. [Google Scholar] [CrossRef] [Green Version]
  17. Gunter, T.E.; Buntinas, L.; Sparagna, G.; Eliseev, R.; Gunter, K. Mitochondrial calcium transport: Mechanisms and functions. Cell Calcium 2000, 28, 285–296. [Google Scholar] [CrossRef]
  18. Maechler, P. Mitochondria as the conductor of metabolic signals for insulin exocytosis in pancreatic β-cells. Cell. Mol. Life Sci. 2002, 59, 1803–1818. [Google Scholar] [CrossRef] [PubMed]
  19. Medler, K.F. Calcium signaling in taste cells: Regulation required. Chem. Senses 2010, 35, 753–765. [Google Scholar] [CrossRef] [Green Version]
  20. Knyazeva, T.A.; Malyugin, E.F.; Zarinskaya, S.A.; Archakov, A.I. Solubilization of cytochrome c in ischemic liver tissue. Vopr. Meditsinskoi Khimii 1975, 21, 481–485. [Google Scholar] [PubMed]
  21. Loeffler, M.; Kroemer, G. The mitochondrion in cell death control: Certainties and incognita. Exp. Cell Res. 2000, 256, 19–26. [Google Scholar] [CrossRef]
  22. Ton, V.K.; Rao, R. Expression of Hailey-Hailey disease mutations in yeast. J. Investig. Dermatol. 2004, 123, 1192–1194. [Google Scholar] [CrossRef] [Green Version]
  23. Pahl, H.L. Signal transduction from the endoplasmic reticulum to the cell nucleus. Physiol. Rev. 1999, 79, 683–701. [Google Scholar] [CrossRef]
  24. Arthington, B.A.; Bennett, L.G.; Skatrud, P.L.; Guynn, C.J.; Barbuch, R.J.; Ulbright, C.E.; Bard, M. Cloning, disruption and sequence of the gene encoding yeast C-5 sterol desaturase. Gene 1991, 102, 39–44. [Google Scholar] [CrossRef]
  25. Lees, N.D.; Skaggs, B.; Kirsch, D.R.; Bard, M. Cloning of the late genes in the ergosterol biosynthetic pathway of Saccharomyces cerevisiae—A review. Lipids 1995, 30, 221–226. [Google Scholar] [CrossRef] [PubMed]
  26. Roth, A.F.; Feng, Y.; Chen, L.Y.; Davis, N.G. The yeast DHHC cysteine-rich domain protein Akr1p is a palmitoyl transferase. J. Cell Biol. 2002, 159, 23–28. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  27. Ducker, C.E.; Stettler, E.M.; French, K.J.; Upson, J.J.; Smith, C.D. Huntingtin interacting protein 14 is an oncogenic human protein: Palmitoyl acyltransferase. Oncogene 2004, 23, 9230–9237. [Google Scholar] [CrossRef] [Green Version]
  28. Huang, K.; Yanai, A.; Kang, R.; Arstikaitis, P.; Singaraja, R.R.; Metzler, M.; Mullard, A.; Haigh, B.; Gauthier-Campbell, C.; Gutekunst, C.A.; et al. Huntingtin-interacting protein HIP14 is a palmitoyl transferase involved in palmitoylation and trafficking of multiple neuronal proteins. Neuron 2004, 44, 977–986. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  29. Carmona-Gutierrez, D.; Eisenberg, T.; Buttner, S.; Meisinger, C.; Kroemer, G.; Madeo, F. Apoptosis in yeast: Triggers, pathways, subroutines. Cell Death Differ. 2010, 17, 763–773. [Google Scholar] [CrossRef] [Green Version]
  30. Zhao, Y.Y.; Du, J.C.; Zhao, G.; Jiang, L.H. Activation of calcineurin is mainly responsible for the calcium sensitivity of gene deletion mutations in the genome of budding yeast. Genomics 2013, 101, 49–56. [Google Scholar] [CrossRef] [Green Version]
  31. Winzeler, E.A.; Shoemaker, D.D.; Astromoff, A.; Liang, H.; Anderson, K.; Andre, B.; Bangham, R.; Benito, R.; Boeke, J.D.; Bussey, H.; et al. Functional characterization of the S. cerevisiae genome by gene deletion and parallel analysis. Science 1999, 285, 901–906. [Google Scholar] [CrossRef] [Green Version]
  32. Zhao, Y.Y.; Yan, H.B.; Happeck, R.; Peiter-Volk, T.; Xu, H.H.; Zhang, Y.; Peiter, E.; Triplet, C.V.; Whiteway, M.; Jiang, L.H. The plasma membrane protein Rch1 is a negative regulator of cytosolic calcium homeostasis and positively regulated by the calcium/calcineurin signaling pathway in budding yeast. Eur. J. Cell Biol. 2016, 95, 164–174. [Google Scholar] [CrossRef]
  33. Longtine, M.S.; McKenzie, A.; Demarini, D.J.; Shah, N.G.; Wach, A.; Brachat, A.; Philippsen, P.; Pringle, J.R. Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae. Yeast 1998, 14, 953–961. [Google Scholar] [CrossRef]
  34. Zhao, Y.; Du, J.; Xiong, B.; Xu, H.; Jiang, L. ESCRT components regulate the expression of the ER/Golgi calcium pump gene PMR1 through the Rim101/Nrg1 pathway in budding yeast. J. Mol. Cell Biol. 2013, 5, 336–344. [Google Scholar] [CrossRef] [Green Version]
  35. Madeo, F.; Frohlich, E.; Frohlich, K.U. A yeast mutant showing diagnostic markers of early and late apoptosis. J. Cell Biol. 1997, 139, 729–734. [Google Scholar] [CrossRef] [Green Version]
  36. Cao, C.; Cao, Z.; Yu, P.; Zhao, Y. Genome-wide identification for genes involved in sodium dodecyl sulfate toxicity in Saccharomyces cerevisiae. BMC Microbiol. 2020, 20, 34. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  37. Buttner, S.; Eisenberg, T.; Carmona-Gutierrez, D.; Ruli, D.; Knauer, H.; Ruckenstuhl, C.; Sigrist, C.; Wissing, S.; Kollroser, M.; Frohlich, K.U.; et al. Endonuclease G regulates budding yeast life and death. Mol. Cell 2007, 25, 233–246. [Google Scholar] [CrossRef]
  38. Kohrer, K.; Domdey, H. Preparation of high molecular weight RNA. Methods Enzymol. 1991, 194, 398–405. [Google Scholar] [PubMed]
  39. Livak, K.J.; Schmittgen, T.D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods 2001, 25, 402–408. [Google Scholar] [CrossRef]
  40. Batiza, A.F.; Schulz, T.; Masson, P.H. Yeast respond to hypotonic shock with a calcium pulse. J. Biol. Chem. 1996, 271, 23357–23362. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  41. Peiter, E.; Sun, J.; Heckmann, A.B.; Venkateshwaran, M.; Riely, B.K.; Otegui, M.S.; Edwards, A.; Freshour, G.; Hahn, M.G.; Cook, D.R.; et al. The Medicago truncatula DMI1 protein modulates cytosolic calcium signaling. Plant Physiol. 2007, 145, 192–203. [Google Scholar] [CrossRef] [Green Version]
  42. Babu, P.; Deschenes, R.J.; Robinson, L.C. Akr1p-dependent palmitoylation of Yck2p yeast casein kinase 1 is necessary and sufficient for plasma membrane targeting. J. Biol. Chem. 2004, 279, 27138–27147. [Google Scholar] [CrossRef] [Green Version]
  43. Moriya, H.; Johnston, M. Glucose sensing and signaling in Saccharomyces cerevisiae through the Rgt2 glucose sensor and casein kinase I. Proc. Natl. Acad. Sci. USA 2004, 101, 1572–1577. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  44. Pasula, S.; Chakraborty, S.; Choi, J.H.; Kim, J.H. Role of casein kinase 1 in the glucose sensor-mediated signaling pathway in yeast. BMC Cell Biol. 2010, 11, 17. [Google Scholar] [CrossRef] [Green Version]
  45. Lewis, D.A.; Bisson, L.F. The Hxt1 gene-product of Saccharomyces-Cerevisiae is a new member of the family of hexose transporters. Mol. Cell. Biol. 1991, 11, 3804–3813. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  46. Roy, A.; Kim, Y.B.; Cho, K.H.; Kim, J.H. Glucose starvation-induced turnover of the yeast glucose transporter Hxt1. Biochim. Biophys. Acta 2014, 1840, 2878–2885. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  47. Bernard, F.; Andre, B. Genetic analysis of the signalling pathway activated by external amino acids in Saccharomyces cerevisiae. Mol. Microbiol. 2001, 41, 489–502. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  48. Schreve, J.L.; Sin, J.K.; Garrett, J.M. The Saccharomyces cerevisiae YCC5 (YCL025c) gene encodes an amino acid permease, Agp1, which transports asparagine and glutamine. J. Bacteriol. 1998, 180, 2556–2559. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  49. Liu, Z.; Thornton, J.; Spirek, M.; Butow, R.A. Activation of the SPS amino acid-sensing pathway in Saccharomyces cerevisiae correlates with the phosphorylation state of a sensor component, Ptr3. Mol. Cell. Biol. 2008, 28, 551–563. [Google Scholar] [CrossRef] [Green Version]
  50. Reddi, A.R.; Culotta, V.C. SOD1 integrates signals from oxygen and glucose to repress respiration. Cell 2013, 152, 224–235. [Google Scholar] [CrossRef] [Green Version]
  51. Huh, W.K.; Falvo, J.V.; Gerke, L.C.; Carroll, A.S.; Howson, R.W.; Weissman, J.S.; O’Shea, E.K. Global analysis of protein localization in budding yeast. Nature 2003, 425, 686–691. [Google Scholar] [CrossRef]
  52. Ozcan, S.; Johnston, M. Function and regulation of yeast hexose transporters. Microbiol. Mol. Biol. Rev. 1999, 63, 554–569. [Google Scholar] [CrossRef] [Green Version]
  53. Belotti, F.; Tisi, R.; Paiardi, C.; Rigamonti, M.; Groppi, S.; Martegani, E. Localization of Ras signaling complex in budding yeast. Biochim. Biophys. Acta 2012, 1823, 1208–1216. [Google Scholar] [CrossRef] [Green Version]
  54. Rolland, F.; Winderickx, J.; Thevelein, J.M. Glucose-sensing and -signalling mechanisms in yeast. FEMS Yeast Res. 2002, 2, 183–201. [Google Scholar] [CrossRef]
  55. Johnston, M.; Kim, J.H. Glucose as a hormone: Receptor-mediated glucose sensing in the yeast Saccharomyces cerevisiae. Biochem. Soc. Trans. 2005, 33, 247–252. [Google Scholar] [CrossRef] [Green Version]
  56. Kim, J.H.; Roy, A.; Jouandot, D., 2nd; Cho, K.H. The glucose signaling network in yeast. Biochim. Biophys. Acta 2013, 1830, 5204–5210. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  57. Tomas-Cobos, L.; Viana, R.; Sanz, P. TOR kinase pathway and 14-3-3 proteins regulate glucose-induced expression of HXT1, a yeast low-affinity glucose transporter. Yeast 2005, 22, 471–479. [Google Scholar] [CrossRef] [Green Version]
  58. Souza, A.A.; Miranda, M.N.; da Silva, S.F.; Bozaquel-Morais, B.; Masuda, C.A.; Ghislain, M.; Montero-Lomeli, M. Expression of the glucose transporter HXT1 involves the Ser-Thr protein phosphatase Sit4 in Saccharomyces cerevisiae. FEMS Yeast Res. 2012, 12, 907–917. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  59. Roy, A.; Dement, A.D.; Cho, K.H.; Kim, J.H. Assessing glucose uptake through the yeast hexose transporter 1 (Hxt1). PLoS ONE 2015, 10, e0121985. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  60. Tanahashi, R.; Matsushita, T.; Nishimura, A.; Takagi, H. Downregulation of the broad-specificity amino acid permease Agp1 mediated by the ubiquitin ligase Rsp5 and the arrestin-like protein Bul1 in yeast. Biosci. Biotechnol. Biochem. 2021, 85, 1266–1274. [Google Scholar] [CrossRef]
  61. Hsiung, Y.G.; Chang, H.C.; Pellequer, J.L.; La Valle, R.; Lanker, S.; Wittenberg, C. F-box protein Grr1 interacts with phosphorylated targets via the cationic surface of its leucine-rich repeat. Mol. Cell. Biol. 2001, 21, 2506–2520. [Google Scholar] [CrossRef] [Green Version]
  62. Iraqui, I.; Vissers, S.; Bernard, F.; de Craene, J.O.; Boles, E.; Urrestarazu, A.; Andre, B. Amino acid signaling in Saccharomyces cerevisiae: A permease-like sensor of external amino acids and F-Box protein Grr1p are required for transcriptional induction of the AGP1 gene, which encodes a broad-specificity amino acid permease. Mol. Cell. Biol. 1999, 19, 989–1001. [Google Scholar] [CrossRef] [Green Version]
  63. Stathopoulos, A.M.; Cyert, M.S. Calcineurin acts through the CRZ1/TCN1-encoded transcription factor to regulate gene expression in yeast. Genes Dev. 1997, 11, 3432–3444. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  64. Matheos, D.P.; Kingsbury, T.J.; Ahsan, U.S.; Cunningham, K.W. Tcn1p/Crz1p a calcineurin-dependent transcription factor that differentially regulates gene expression in Saccharomyces cerevisiae. Genes Dev. 1997, 11, 3445–3458. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  65. Bastajian, N.; Friesen, H.; Andrews, B.J. Bck2 Acts through the MADS box protein mcm1 to activate cell-cycle-regulated genes in budding yeast. PLoS Genet. 2013, 9, e1003507. [Google Scholar] [CrossRef] [Green Version]
  66. Cyert, M.S. Calcineurin signaling in Saccharomyces cereviside: How yeast go crazy in response to stress. Biochem. Biophys. Res. Commun. 2003, 311, 1143–1150. [Google Scholar] [CrossRef]
Genes 12 01311 g001 550
Figure 1. Calcium inhibits elevated ROS levels and cell death in akr1∆ and erg3∆ mutants. (A) Intracellular ROS levels in wild-type (WT) BY4741, akr1∆ and erg3∆ strains with or without calcium treatment. (B) Cell death in WT BY4741, akr1∆ and erg3∆ strains with or without calcium treatment. Results were analysed using paired-samples t-test function of SPSS 19.0. The significant difference of p < 0.01 is showed as “*” or “#” when cells were treated without or with calcium, respectively.
Figure 1. Calcium inhibits elevated ROS levels and cell death in akr1∆ and erg3∆ mutants. (A) Intracellular ROS levels in wild-type (WT) BY4741, akr1∆ and erg3∆ strains with or without calcium treatment. (B) Cell death in WT BY4741, akr1∆ and erg3∆ strains with or without calcium treatment. Results were analysed using paired-samples t-test function of SPSS 19.0. The significant difference of p < 0.01 is showed as “*” or “#” when cells were treated without or with calcium, respectively.
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Genes 12 01311 g002 550
Figure 2. Mutants of AKR1 and ERG3 cannot recover cytosolic localisation of Yck1-GFP and Yck2-GFP. Wild-type BY4741, akr1∆ and erg3∆ cells expressing GFP-tagged Yck1 or Yck2 were grown to early log phase in YPD medium then were treated with 0.2 M CaCl2. Cells were visualised under a Nikon ECLIPSE 80i fluorescence microscope.
Figure 2. Mutants of AKR1 and ERG3 cannot recover cytosolic localisation of Yck1-GFP and Yck2-GFP. Wild-type BY4741, akr1∆ and erg3∆ cells expressing GFP-tagged Yck1 or Yck2 were grown to early log phase in YPD medium then were treated with 0.2 M CaCl2. Cells were visualised under a Nikon ECLIPSE 80i fluorescence microscope.
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Genes 12 01311 g003 550
Figure 3. (A,B) β-galactosidase activity of HXT1-lacZ and AGP1-lacZ in WT, akr1Δ, erg3Δ and crz1Δ strains in response to 0.2 M CaCl2. Data are the means of six independent experiments. (C,D) Relative expression levels of HXT1 and AGP1 genes in response to 0.2 M CaCl2. WT, akr1Δ and erg3Δ strains were treated with 0.2 M CaCl2 for 2 h and expression of the indicated genes was measured by qRT-PCR. Values are averages of three independent assays for each strain. Results were analysed using paired-samples t-test function of SPSS 19.0. The significant difference of p < 0.01 is showed as “*” or “#” when cells were treated without or with calcium, respectively.
Figure 3. (A,B) β-galactosidase activity of HXT1-lacZ and AGP1-lacZ in WT, akr1Δ, erg3Δ and crz1Δ strains in response to 0.2 M CaCl2. Data are the means of six independent experiments. (C,D) Relative expression levels of HXT1 and AGP1 genes in response to 0.2 M CaCl2. WT, akr1Δ and erg3Δ strains were treated with 0.2 M CaCl2 for 2 h and expression of the indicated genes was measured by qRT-PCR. Values are averages of three independent assays for each strain. Results were analysed using paired-samples t-test function of SPSS 19.0. The significant difference of p < 0.01 is showed as “*” or “#” when cells were treated without or with calcium, respectively.
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Genes 12 01311 g004 550
Figure 4. Overexpression of HXT1 or AGP1 could inhibit the increased intracellular ROS levels in akr1∆ and erg3∆ mutants. (A) Intracellular ROS levels in wild-type (WT) BY4741, and akr1∆ and erg3∆ mutants overexpressing HXT1. (B) Intracellular ROS levels in wild-type (WT) BY4741, and akr1∆ and erg3∆ mutants overexpressing AGP1. Results were analysed using paired-samples t-test function of SPSS 19.0. The significant difference of p < 0.01 is showed as “*” or “#” when cells were treated with or without calcium.
Figure 4. Overexpression of HXT1 or AGP1 could inhibit the increased intracellular ROS levels in akr1∆ and erg3∆ mutants. (A) Intracellular ROS levels in wild-type (WT) BY4741, and akr1∆ and erg3∆ mutants overexpressing HXT1. (B) Intracellular ROS levels in wild-type (WT) BY4741, and akr1∆ and erg3∆ mutants overexpressing AGP1. Results were analysed using paired-samples t-test function of SPSS 19.0. The significant difference of p < 0.01 is showed as “*” or “#” when cells were treated with or without calcium.
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Figure 5. Cytosolic Ca2+ levels are decreased in akr1Δ and erg3Δ strains. WT, akr1Δ and erg3Δ strains harbouring the pEVP11-AEQ plasmid were used to monitor cytosolic Ca2+ concentrations. Data are the means of three independent experiments.
Figure 5. Cytosolic Ca2+ levels are decreased in akr1Δ and erg3Δ strains. WT, akr1Δ and erg3Δ strains harbouring the pEVP11-AEQ plasmid were used to monitor cytosolic Ca2+ concentrations. Data are the means of three independent experiments.
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Figure 6. Expression levels of AKR1 and ERG3 are regulated by the calcium/calcineurin pathway. (A) Relative expression levels of the AKR1 gene in response to 0.2 M CaCl2. (B) Relative expression levels of the ERG3 gene in response to 0.2 M CaCl2. WT and crz1Δ strains were treated with 0.2 M CaCl2 for 2 h and expression of the indicated genes was measured by qRT-PCR. Values are averages of three independent assays for each strain. (C) Subcellular localisation of Erg3-GFP and Akr1-GFP proteins in response to 0.2 M CaCl2. Wildtype BY4741 cells expressing Erg3-GFP or Akr1-GFP were grown to early log phase in YPD medium then treated with 0.2 M CaCl2. Cells were visualised under a Nikon ECLIPSE 80i fluorescence microscope. Results were analysed using paired-samples t-test function of SPSS 19.0. The significant difference of p < 0.01 is showed as “*” or “#” when cells were treated without or with calcium, respectively.
Figure 6. Expression levels of AKR1 and ERG3 are regulated by the calcium/calcineurin pathway. (A) Relative expression levels of the AKR1 gene in response to 0.2 M CaCl2. (B) Relative expression levels of the ERG3 gene in response to 0.2 M CaCl2. WT and crz1Δ strains were treated with 0.2 M CaCl2 for 2 h and expression of the indicated genes was measured by qRT-PCR. Values are averages of three independent assays for each strain. (C) Subcellular localisation of Erg3-GFP and Akr1-GFP proteins in response to 0.2 M CaCl2. Wildtype BY4741 cells expressing Erg3-GFP or Akr1-GFP were grown to early log phase in YPD medium then treated with 0.2 M CaCl2. Cells were visualised under a Nikon ECLIPSE 80i fluorescence microscope. Results were analysed using paired-samples t-test function of SPSS 19.0. The significant difference of p < 0.01 is showed as “*” or “#” when cells were treated without or with calcium, respectively.
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Table
Table 1. Strains used in this study.
Table 1. Strains used in this study.
NameRelevant GenotypeSource/Reference
BY4741MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0[31]
akr1ΔBY4741 akr1::kanMX4[31]
erg3ΔBY4741 erg3::kanMX4[31]
sod1ΔBY4741 sod1::kanMX4[31]
crz1ΔBY4741 crz1::kanMX4[31]
BY4741 GFP-YCK1BY4741 HIS3MX6- GFP-YCK1This study
akr1Δ GFP-YCK1BY4741 akr1::kanMX4 HIS3MX6-GFP-YCK1This study
erg3Δ GFP-YCK1BY4741 erg3::kanMX4 HIS3MX6-GFP-YCK1This study
BY4741 GFP-YCK2BY4741 HIS3MX6-GFP-YCK2This study
akr1Δ GFP-YCK2BY4741 akr1::kanMX4 HIS3MX6-GFP-YCK2This study
erg3Δ GFP-YCK2BY4741 erg3::kanMX4 HIS3MX6-GFP-YCK2This study
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Li, G.; Fu, W.; Deng, Y.; Zhao, Y. Role of Calcium/Calcineurin Signalling in Regulating Intracellular Reactive Oxygen Species Homeostasis in Saccharomyces cerevisiae. Genes 2021, 12, 1311. https://doi.org/10.3390/genes12091311

AMA Style

Li G, Fu W, Deng Y, Zhao Y. Role of Calcium/Calcineurin Signalling in Regulating Intracellular Reactive Oxygen Species Homeostasis in Saccharomyces cerevisiae. Genes. 2021; 12(9):1311. https://doi.org/10.3390/genes12091311

Chicago/Turabian Style

Li, Guohui, Wenxuan Fu, Yu Deng, and Yunying Zhao. 2021. "Role of Calcium/Calcineurin Signalling in Regulating Intracellular Reactive Oxygen Species Homeostasis in Saccharomyces cerevisiae" Genes 12, no. 9: 1311. https://doi.org/10.3390/genes12091311

APA Style

Li, G., Fu, W., Deng, Y., & Zhao, Y. (2021). Role of Calcium/Calcineurin Signalling in Regulating Intracellular Reactive Oxygen Species Homeostasis in Saccharomyces cerevisiae. Genes, 12(9), 1311. https://doi.org/10.3390/genes12091311

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