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Article

Establishment of an Efficient In Vitro Propagation of Cnidium officinale Makino and Selection of Superior Clones through Flow Cytometric Assessment of DNA Content

by
Hyung-Eun Kim
1,2,
Jong-Eun Han
1,
Hyoshin Lee
3,
Hosakatte Niranjana Murthy
1,4,
Hyuk-Joon Kwon
5,
Gun-Myung Lee
5 and
So-Young Park
1,*
1
Department of Horticultural Science, Chungbuk National University, Cheongju 28644, Korea
2
Korea Disease Control and Prevention Agency, Osong-eup, Cheongju 28159, Korea
3
Department of Forest Genetic Resources, National Institute of Forest Science, 39 Onjeong-ro, Suwon 16631, Korea
4
Department of Botany, Karnatak University, Dharwad 580003, India
5
Food Science R&D Center, Kolmar BNH Co., Seocho-gu, Seoul 30003, Korea
*
Author to whom correspondence should be addressed.
Genes 2022, 13(10), 1815; https://doi.org/10.3390/genes13101815
Submission received: 19 September 2022 / Revised: 6 October 2022 / Accepted: 6 October 2022 / Published: 8 October 2022
(This article belongs to the Special Issue Phylogenetics, Genetics, and Breeding of Medicinal Plants)
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Abstract

Cnidium officinale is a valuable medicinal plant cultivated in Asia for its rhizomes. This study reports the in vitro regeneration of Cnidium officinale plants and the induction of rhizomes from microshoots. The rhizomatous buds of Cnidium officinale induced multiple shoots on Murashige and Skoog (MS) medium supplemented with 0.5 mg L−1 BA, which led to the regeneration of plants within four weeks of culture. After four weeks of culture, the plants were assessed for fresh weight, the number of leaves, the number of roots, and the length of roots to compare the performance of the different clones. The clones with good growth characteristics were selected with the aid of a flow cytometric analysis of 2C nuclear DNA content. The plants bearing high DNA values showed better growth characteristics. Various factors, namely, sucrose concentration (30, 50, 70, and 90 g L−1), ABA (0, 0.5, 1.0, and 2.0 mg L−1), the synergistic effects of BA (1.0 mg L−1) + NAA (0.5 mg L−1) and BA (1.0 mg L−1) + NAA (0.5 mg L−1) + ABA (1.0 mg L−1) with or without activated charcoal (1 g L−1), and light and dark incubation were tested on rhizome formation from microshoots. The results of the above experiments suggest that MS medium supplemented with 50 g L−1 sucrose, 1.0 mg L−1 ABA, and 1 g L−1 AC is good for the induction of rhizomes from the shoots of Cnidium officinale. Plantlets with rhizomes were successfully transferred to pots, and they showed 100% survival.
Keywords: flow cytometry; medicinal plant; multiple shoots; plant regeneration; rhizomes flow cytometry; medicinal plant; multiple shoots; plant regeneration; rhizomes

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MDPI and ACS Style

Kim, H.-E.; Han, J.-E.; Lee, H.; Murthy, H.N.; Kwon, H.-J.; Lee, G.-M.; Park, S.-Y. Establishment of an Efficient In Vitro Propagation of Cnidium officinale Makino and Selection of Superior Clones through Flow Cytometric Assessment of DNA Content. Genes 2022, 13, 1815. https://doi.org/10.3390/genes13101815

AMA Style

Kim H-E, Han J-E, Lee H, Murthy HN, Kwon H-J, Lee G-M, Park S-Y. Establishment of an Efficient In Vitro Propagation of Cnidium officinale Makino and Selection of Superior Clones through Flow Cytometric Assessment of DNA Content. Genes. 2022; 13(10):1815. https://doi.org/10.3390/genes13101815

Chicago/Turabian Style

Kim, Hyung-Eun, Jong-Eun Han, Hyoshin Lee, Hosakatte Niranjana Murthy, Hyuk-Joon Kwon, Gun-Myung Lee, and So-Young Park. 2022. "Establishment of an Efficient In Vitro Propagation of Cnidium officinale Makino and Selection of Superior Clones through Flow Cytometric Assessment of DNA Content" Genes 13, no. 10: 1815. https://doi.org/10.3390/genes13101815

APA Style

Kim, H.-E., Han, J.-E., Lee, H., Murthy, H. N., Kwon, H.-J., Lee, G.-M., & Park, S.-Y. (2022). Establishment of an Efficient In Vitro Propagation of Cnidium officinale Makino and Selection of Superior Clones through Flow Cytometric Assessment of DNA Content. Genes, 13(10), 1815. https://doi.org/10.3390/genes13101815

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