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Article

Genomic and Transcriptomic Characterization of Atypical Recurrent Flank Alopecia in the Cesky Fousek

by
Silvie Neradilová
1,
Alexandria M. Schauer
2,3,
Jessica J. Hayward
4,
Magdalena A. T. Brunner
2,3,
Magdalena Bohutínská
5,
Vidhya Jagannathan
6,
Laurie B. Connell
7,
Adam R. Boyko
4,†,
Monika M. Welle
2,3,† and
Barbora Černá Bolfíková
1,*,†
1
Department of Animal Science and Food Processing, Faculty of Tropical AgriSciences, Czech University of Life Sciences Prague, 16500 Prague, Czech Republic
2
Institute of Animal Pathology, Vetsuisse Faculty, University of Bern, 3012 Bern, Switzerland
3
DermFocus, University of Bern, 3012 Bern, Switzerland
4
Department of Biomedical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
5
Department of Botany, Faculty of Science, Charles University, 12800 Prague, Czech Republic
6
Institute of Genetics, Vetsuisse Faculty, University of Bern, 3012 Bern, Switzerland
7
Molecular & Biomedical Sciences and School of Marine Sciences, University of Maine, Orono, ME 04469, USA
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Genes 2022, 13(4), 650; https://doi.org/10.3390/genes13040650
Submission received: 20 February 2022 / Revised: 3 April 2022 / Accepted: 5 April 2022 / Published: 7 April 2022
(This article belongs to the Special Issue Canine Genetics 2)
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Abstract

Non-inflammatory alopecia is a frequent skin problem in dogs, causing damaged coat integrity and compromised appearance of affected individuals. In this study, we examined the Cesky Fousek breed, which displays atypical recurrent flank alopecia (aRFA) at a high frequency. This type of alopecia can be quite severe and is characterized by seasonal episodes of well demarcated alopecic areas without hyperpigmentation. The genetic component responsible for aRFA remains unknown. Thus, here we aimed to identify variants involved in aRFA using a combination of histological, genomic, and transcriptomic data. We showed that aRFA is histologically similar to recurrent flank alopecia, characterized by a lack of anagen hair follicles and the presence of severely shortened telogen or kenogen hair follicles. We performed a genome-wide association study (GWAS) using 216 dogs phenotyped for aRFA and identified associations on chromosomes 19, 8, 30, 36, and 21, highlighting 144 candidate genes, which suggests a polygenic basis for aRFA. By comparing the skin cell transcription pattern of six aRFA and five control dogs, we identified 236 strongly differentially expressed genes (DEGs). We showed that the GWAS genes associated with aRFA are often predicted to interact with DEGs, suggesting their joint contribution to the development of the disease. Together, these genes affect four major metabolic pathways connected to aRFA: collagen formation, muscle structure/contraction, lipid metabolism, and the immune system.
Keywords: dog; GWAS; canine alopecia; atypical recurrent flank alopecia; Cesky Fousek; RNA-seq; differential gene expression; skin biopsies dog; GWAS; canine alopecia; atypical recurrent flank alopecia; Cesky Fousek; RNA-seq; differential gene expression; skin biopsies

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MDPI and ACS Style

Neradilová, S.; Schauer, A.M.; Hayward, J.J.; Brunner, M.A.T.; Bohutínská, M.; Jagannathan, V.; Connell, L.B.; Boyko, A.R.; Welle, M.M.; Černá Bolfíková, B. Genomic and Transcriptomic Characterization of Atypical Recurrent Flank Alopecia in the Cesky Fousek. Genes 2022, 13, 650. https://doi.org/10.3390/genes13040650

AMA Style

Neradilová S, Schauer AM, Hayward JJ, Brunner MAT, Bohutínská M, Jagannathan V, Connell LB, Boyko AR, Welle MM, Černá Bolfíková B. Genomic and Transcriptomic Characterization of Atypical Recurrent Flank Alopecia in the Cesky Fousek. Genes. 2022; 13(4):650. https://doi.org/10.3390/genes13040650

Chicago/Turabian Style

Neradilová, Silvie, Alexandria M. Schauer, Jessica J. Hayward, Magdalena A. T. Brunner, Magdalena Bohutínská, Vidhya Jagannathan, Laurie B. Connell, Adam R. Boyko, Monika M. Welle, and Barbora Černá Bolfíková. 2022. "Genomic and Transcriptomic Characterization of Atypical Recurrent Flank Alopecia in the Cesky Fousek" Genes 13, no. 4: 650. https://doi.org/10.3390/genes13040650

APA Style

Neradilová, S., Schauer, A. M., Hayward, J. J., Brunner, M. A. T., Bohutínská, M., Jagannathan, V., Connell, L. B., Boyko, A. R., Welle, M. M., & Černá Bolfíková, B. (2022). Genomic and Transcriptomic Characterization of Atypical Recurrent Flank Alopecia in the Cesky Fousek. Genes, 13(4), 650. https://doi.org/10.3390/genes13040650

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