Heterologous Expression of Platycodon grandiflorus PgF3′5′H Modifies Flower Color Pigmentation in Tobacco

Ma, Lulin; Jia, Wenjie; Duan, Qing; Du, Wenwen; Li, Xiang; Cui, Guangfen; Wang, Xiangning; Wang, Jihua

doi:10.3390/genes14101920

Open AccessArticle

Heterologous Expression of Platycodon grandiflorus PgF3′5′H Modifies Flower Color Pigmentation in Tobacco

by

Lulin Ma

,

Wenjie Jia

,

Qing Duan

,

Wenwen Du

,

Xiang Li

,

Guangfen Cui

,

Xiangning Wang

and

Jihua Wang

^*

Flower Research Institute of Yunnan Academy of Agricultural Sciences, Key Lab of Yunnan Flower Breeding, National Engineering Research Center For Ornamental Horticulture, Kunming 650205, China

^*

Author to whom correspondence should be addressed.

Genes 2023, 14(10), 1920; https://doi.org/10.3390/genes14101920

Submission received: 1 September 2023 / Revised: 28 September 2023 / Accepted: 4 October 2023 / Published: 9 October 2023

(This article belongs to the Special Issue 5Gs in Crop Genetic and Genomic Improvement)

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Abstract

Flavonoid-3′,5′-hydroxylase (F3′5′H) is the key enzyme for the biosynthesis of delphinidin-based anthocyanins, which are generally required for purple or blue flowers. Previously, we isolated a full-length cDNA of PgF3′5′H from Platycodon grandiflorus, which shared the highest homology with Campanula medium F3′5′H. In this study, PgF3′5′H was subcloned into a plant over-expression vector and transformed into tobacco via Agrobacterium tumefaciens to investigate its catalytic function. Positive transgenic tobacco T₀ plants were obtained by hygromycin resistance screening and PCR detection. PgF3′5′H showed a higher expression level in all PgF3′5′H transgenic tobacco plants than in control plants. Under the drive of the cauliflower mosaic virus (CaMV) 35S promoter, the over-expressed PgF3′5′H produced dihydromyricetin (DHM) and some new anthocyanin pigments (including delphinidin, petunidin, peonidin, and malvidin derivatives), and increased dihydrokaempferol (DHK), taxifolin, tridactyl, cyanidin derivatives, and pelargonidin derivatives in PgF3′5′H transgenic tobacco plants by ultra-performance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS) analysis, resulting in a dramatic color alteration from light pink to magenta. These results indicate that PgF3′5′H products have F3′5′H enzyme activity. In addition, PgF3′5′H transfer alters flavonoid pigment synthesis and accumulation in tobacco. Thus, PgF3′5′H may be considered a candidate gene for gene engineering to enhance anthocyanin accumulation and the molecular breeding project for blue flowers.

Keywords: PgF3′5′H; transgenic tobacco; over-expression; delphinidin-based anthocyanin; blue flower color

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MDPI and ACS Style

Ma, L.; Jia, W.; Duan, Q.; Du, W.; Li, X.; Cui, G.; Wang, X.; Wang, J. Heterologous Expression of Platycodon grandiflorus PgF3′5′H Modifies Flower Color Pigmentation in Tobacco. Genes 2023, 14, 1920. https://doi.org/10.3390/genes14101920

AMA Style

Ma L, Jia W, Duan Q, Du W, Li X, Cui G, Wang X, Wang J. Heterologous Expression of Platycodon grandiflorus PgF3′5′H Modifies Flower Color Pigmentation in Tobacco. Genes. 2023; 14(10):1920. https://doi.org/10.3390/genes14101920

Chicago/Turabian Style

Ma, Lulin, Wenjie Jia, Qing Duan, Wenwen Du, Xiang Li, Guangfen Cui, Xiangning Wang, and Jihua Wang. 2023. "Heterologous Expression of Platycodon grandiflorus PgF3′5′H Modifies Flower Color Pigmentation in Tobacco" Genes 14, no. 10: 1920. https://doi.org/10.3390/genes14101920

APA Style

Ma, L., Jia, W., Duan, Q., Du, W., Li, X., Cui, G., Wang, X., & Wang, J. (2023). Heterologous Expression of Platycodon grandiflorus PgF3′5′H Modifies Flower Color Pigmentation in Tobacco. Genes, 14(10), 1920. https://doi.org/10.3390/genes14101920

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Heterologous Expression of Platycodon grandiflorus PgF3′5′H Modifies Flower Color Pigmentation in Tobacco

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