Nanoparticle-Mediated Genetic Transformation in a Selaginella Species
Abstract
:1. Introduction
2. Materials and Methods
2.1. Synthesis of Arginine-Functionalized Nanohydroxyapatite Particles
2.2. Plant Growth
2.3. The Cloning and Plasmid Isolation of the G3GFP-GUS Fusion Construct
2.4. Preparation of pGWB452-G3GFP::GUS|R-nHA Conjugate Solution
2.5. Transient Transformation of pGWB452-G3GFP::GUS|R-nHA Conjugates in S. moellendorffii
2.6. Determination of GFP or GUS Reporter Gene Expression
2.7. The Plasmid Isolation of the pAXY001 Expression Clone That Contains the eYGFPuv Gene
2.8. Preparation of pAXY001|R-nHA Conjugate Solution and Transient Transformation of pAXY001|R-nHA Conjugates into S. moellendorffii
2.9. Agrobacterium-Mediated Delivery of GUS Reporter Gene
2.10. Characterization of Conjugates
2.10.1. Transmission Electron Microscopy Imaging
2.10.2. Zeta Potential Measurements
3. Results
3.1. Arginine-Functionalized Nanohydroxyapatite Particle-Mediated Expression of GFP or GUS Reporter Genes in S. moellendorffii Sporophylls
3.2. Arginine-Functionalized Nanohydroxyapatite Particle-Mediated Expression of eYGFPuv Reporter Gene in S. moellendorffii Sporophylls
3.3. Agrobacterium-Mediated Transient Expression of GUS in S. moellendorffii Sporophylls
3.4. Characterization of Conjugates
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Ariyarathne, M.A.; Wone, B.; Wijewantha, N.; Wone, B.W.M. Nanoparticle-Mediated Genetic Transformation in a Selaginella Species. Genes 2024, 15, 1091. https://doi.org/10.3390/genes15081091
Ariyarathne MA, Wone B, Wijewantha N, Wone BWM. Nanoparticle-Mediated Genetic Transformation in a Selaginella Species. Genes. 2024; 15(8):1091. https://doi.org/10.3390/genes15081091
Chicago/Turabian StyleAriyarathne, Madhavi A., Beate Wone, Nisitha Wijewantha, and Bernard W. M. Wone. 2024. "Nanoparticle-Mediated Genetic Transformation in a Selaginella Species" Genes 15, no. 8: 1091. https://doi.org/10.3390/genes15081091