Histone Arginine Methylation as a Regulator of Gene Expression in the Dehydrating African Clawed Frog (Xenopus laevis)
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animal Treatments and Tissues
2.2. Total Protein Extraction
2.3. Electrophoresis
2.4. Immunoblotting
2.5. Data Quantification
3. Results
3.1. Protein Arginine Methyl Transferases
3.2. Methylated Histone Residues
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Appendix A
Antibody | Company | Catalogue # |
---|---|---|
PRMT1 | Genetex | GTX128211 |
PRMT2 | Santa Cruz | sc-393254 |
PRMT3 | Genetex | GTX116478 |
PRMT4 | Active Motif | AB_2793205 |
PRMT5 | Abclonal | A2290 |
PRMT6 | Abclonal | A7814 |
PRMT7 | Abclonal | A12159 |
PRMT8 | Novus | NBP1-55401 |
H3R2m2a | MyBioSource | MBS9402172 |
H3R8m2s | MyBioSource | MB59607605 |
H3R8m2a | MyBioSource | MBS9409769 |
H3R26m2a | Abclonal | A2375 |
H4R3m2a | Abclonal | A2376 |
H4R3m2s | MyBioSource | MBS126223 |
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Rehman, S.; Parent, M.; Storey, K.B. Histone Arginine Methylation as a Regulator of Gene Expression in the Dehydrating African Clawed Frog (Xenopus laevis). Genes 2024, 15, 1156. https://doi.org/10.3390/genes15091156
Rehman S, Parent M, Storey KB. Histone Arginine Methylation as a Regulator of Gene Expression in the Dehydrating African Clawed Frog (Xenopus laevis). Genes. 2024; 15(9):1156. https://doi.org/10.3390/genes15091156
Chicago/Turabian StyleRehman, Saif, Mackenzie Parent, and Kenneth B. Storey. 2024. "Histone Arginine Methylation as a Regulator of Gene Expression in the Dehydrating African Clawed Frog (Xenopus laevis)" Genes 15, no. 9: 1156. https://doi.org/10.3390/genes15091156