Genome-Wide Analysis Reveals the Secondary Metabolome in Streptomyces kanasensis ZX01
Abstract
:1. Introduction
2. Materials and Methods
2.1. Strains, Plasmids and Growth Conditions
2.2. Genome Sequencing, Annotation, and Analysis
2.3. Quantitative Real-time PCR Analysis
2.4. Construction of nsdA-Null Mutant
2.5. Purification and Determination of Glycoprotein GP-1
2.6. Statistical Analyses
3. Results and Discussion
3.1. Sequencing and Gene Annotation of the S. kanasensis ZX01
3.2. Gene Clusters for Secondary Metabolites
3.3. Regulators for Secondary Metabolism in S. kanasensis ZX01 Genome
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Cluster | Type | GenBank ID | Location | Most Similar Known Biosynthetic Gene Cluster | Similarity (%) * | |
---|---|---|---|---|---|---|
From | To | |||||
1 | Siderophore | LNSV01000002 | 180883 | 189602 | Macrotetrolide | 33 |
2 | Siderophore | LNSV01000023 | 1 | 11484 | Desferrioxamine B | 100 |
3 | Siderophore | LNSV01000114 | 10308 | 18860 | - | - |
4 | Terpene | LNSV01000004 | 55721 | 76761 | - | - |
5 | Terpene | LNSV01000004 | 119146 | 140210 | - | - |
6 | Terpene | LNSV01000006 | 39446 | 61665 | - | - |
7 | Terpene | LNSV01000031 | 32044 | 58653 | Hopene | 61 |
8 | Terpene | LNSV01000035 | 41851 | 62261 | - | - |
9 | Terpene | LNSV01000039 | 39913 | 59882 | Isorenieratene | 85 |
10 | Phenazine | LNSV01000013 | 37297 | 57785 | Marinophenazines | 30 |
11 | Phosphonate | LNSV01000030 | 1 | 34405 | Azinomycin B | 6 |
12 | Bacteriocin | LNSV01000030 | 45699 | 57147 | Enduracidin | 6 |
13 | Ectoine | LNSV01000074 | 2911 | 13309 | Ectoine | 100 |
14 | Lassopeptide | LNSV01000003 | 18630 | 41325 | Collismycin A | 7 |
15 | Thiopeptide | LNSV01000094 | 1 | 22641 | Granaticin | 8 |
16 | T1pks | LNSV01000017 | 13669 | 61120 | Monensin | 5 |
17 | T1pks-Otherks | LNSV01000001 | 32321 | 84798 | - | - |
18 | T1pks-Butyrolactone-NRPs | LNSV01000041 | 1 | 58607 | Micromonolactam | 100 |
19 | T3pks | LNSV01000059 | 19531 | 42521 | Merochlorin | 4 |
20 | Otherks-Phenazine | LNSV01000010 | 440 | 49063 | Esmeraldin | 64 |
21 | NRPs | LNSV01000098 | 1 | 22529 | - | - |
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Zhang, G.; Yu, D.; Sang, B.; Feng, J.; Han, L.; Zhang, X. Genome-Wide Analysis Reveals the Secondary Metabolome in Streptomyces kanasensis ZX01. Genes 2017, 8, 346. https://doi.org/10.3390/genes8120346
Zhang G, Yu D, Sang B, Feng J, Han L, Zhang X. Genome-Wide Analysis Reveals the Secondary Metabolome in Streptomyces kanasensis ZX01. Genes. 2017; 8(12):346. https://doi.org/10.3390/genes8120346
Chicago/Turabian StyleZhang, Guoqiang, Dailin Yu, Bu Sang, Juntao Feng, Lirong Han, and Xing Zhang. 2017. "Genome-Wide Analysis Reveals the Secondary Metabolome in Streptomyces kanasensis ZX01" Genes 8, no. 12: 346. https://doi.org/10.3390/genes8120346
APA StyleZhang, G., Yu, D., Sang, B., Feng, J., Han, L., & Zhang, X. (2017). Genome-Wide Analysis Reveals the Secondary Metabolome in Streptomyces kanasensis ZX01. Genes, 8(12), 346. https://doi.org/10.3390/genes8120346