Adsorption Behavior of Nonylphenol on Polystyrene Microplastics and Their Cytotoxicity in Human Caco-2 Cells
Round 1
Reviewer 1 Report
Comments for author File: 
Comments.docx
Author Response
Please see the attachment.
Author Response File: 
Author Response.pdf
Reviewer 2 Report
General comments:
In this paper, the adsorption behavior of nonylphenol (NP) by polystyrene microplastics (PS-MPs) and its cytotoxicity on Caco-2 cells were studied. The structure of the whole article is relatively clear, but there are a lot of detailed errors, such as the image format is not unified, the problem of upper and lower indices, etc., please carefully check and modify. In addition, the conclusion of the article is relatively simple, and the discussion about the research results is shallow. There is a lack of comparison and analysis with the research results of others, and many arguments lack references, with only 28 references in the whole paper, which is not convincing. It is suggested that the manuscript needs a major revision.
Specific comments:
1. Line 154, the format of “CO2” is incorrect, and it should be “CO2”. Please check the format of the upper and lower labels.
2. Line 225, “The composition and characteristic diffraction peaks of PS-MPs corresponded to previous reports”, please add references here. Comparative analysis with previous research results requires literature support.
3. Line 228, please redraw the ruler in the SEM diagram in Figure 1, the current ruler is not very standard and intuitive.
4. Line 243, the numbering form of Figure 2 is different from that before. The numbering form is (1) (2) (3), while the numbering form of Figure 1 before is (a) (b) (c). Please unify the numbering form of pictures in the whole text.
In addition, in Figure 2(1), the qt concentration of the first four particle sizes is quite different from that of the last particle size, which leads to a lot of white space in the figure. It is suggested to interrupt the ordinate “190-192” and “186-187”, which may make the figure more beautiful.
Moreover, it is suggested to make the lines in Figure 2 thicker, because the lines are too thin and not clear at present.
5. Line 269, in Figure 3, why is there only (2) no error bar in the figure?
6. Line 278, please check whether there is a problem with the “R2” format here. Should it be “R2”?
7. Line 287, “3.5. Adso”, please check for errors in “Abso” and modify this title.
8. Line 295, “suggesting that the PS-MPs had homogeneous surface sites and monolayer adsorption was occurring”, please add references, speculative conclusions must be based on references, pay attention to such problems throughout the article.
9. Line 312, it is suggested to modify the color matching in Figure 4. The current color matching is too monotonous, choose some colorful colors to match.
10. Line 325, “3.6.3 Apoptosis”, the position of this Level 3 title is wrong. This is a serious detail error, please check and correct it carefully.
11. Line 355, the combination of (1) and (2) in Figure 6 is not harmonious, so it is suggested to modify the drawing again.
12. Line 375, “difference between the marked groups”, why should the “between” in this sentence be bold? Please correct it. Be sure to check the whole passage carefully for formatting errors.
13. Line 376, “4. Discussion”, this part is not in-depth enough, and the reference is not enough to make the conclusion not strong. The “3. Results” section above also has this problem. The discussion about the results is shallow, and there is a lack of comparative analysis with others’ research results.
14. Line 468, there are only 28 references in the whole paper. It is suggested to have in-depth discussion and supplement the literature where appropriate.
Author Response
Please see the attachment.
Author Response File: Author Response.pdf
Round 2
Reviewer 2 Report
Accept
