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Article

Effects of Diazinon on the Survival, Blood Parameters, Gills, and Liver of Grass Carp (Ctenopharyngodon idella Valenciennes, 1844; Teleostei: Cyprinidae)

by
Sara Vali
1,
Nava Majidiyan
2,
Dariush Azadikhah
3,
Matin Varcheh
4,
Nikola Tresnakova
5 and
Caterina Faggio
6,*
1
Department of Fisheries Sciences, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Science and Natural Resources, Gorgan 49138-15739, Iran
2
Department of Veterinary Medicine, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia 57169-63896, Iran
3
Department of Pathobiology, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia 57169-63896, Iran
4
Department of Chemistry, Faculty of Basic Sciences, Arak Branch, Islamic Azad University, Arak 38361-19131, Iran
5
Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology, Faculty of Fisheries and Protection of Waters, University of South Bohemia in Ceske Budejovice, Zatisi 728/II, 389 25 Vodnany, Czech Republic
6
Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale Ferdinando Stagno D’Alcontres 31, 98166 Messina, Italy
*
Author to whom correspondence should be addressed.
Water 2022, 14(9), 1357; https://doi.org/10.3390/w14091357
Submission received: 13 March 2022 / Revised: 14 April 2022 / Accepted: 20 April 2022 / Published: 22 April 2022
(This article belongs to the Section Biodiversity and Functionality of Aquatic Ecosystems)
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Abstract

:
Diazinon (DZN) is a widely used pesticide that can affect the vital organs of non-target aquatic animals—mainly fish. This study evaluated the acute toxicity (LC50) of six DZN concentrations (0.5, 0.75, 1.5, 2, 2.5, and 3 mg·L−1) and, based on its evaluation after 24 h, 48 h, 72 h, and 96 h, two sublethal concentrations for chronic toxicity testing (0.01 and 0.05 mg·L−1) for 21 days of exposure to DZN on grass carp fingerlings (Ctenopharyngodon idella Valenciennes, 1844). The median lethal concentrations of DZN at 24, 48, 72, and 96 h were 1.83, 1.57, 1.35, and 1.12 mg·L−1, respectively. Next, histological observations after 96 h LC50 showed oedema of the primary lamellae of the gills at low pesticide concentrations (0.5 to 1 mg·L−1) and extensive necrosis of primary lamellae of the gills at higher concentrations (1.5 to 3 mg·L−1). Moreover, cytoplasmic vacuolation and extensive necrosis were observed in liver tissue. Increased skin mucus, unbalanced swimming on the water surface, and increased gill opercula movements were noted during chronic exposure. Haematological parameters such as haematocrit, red blood cell count, white blood cell count, haemoglobin, and mean corpuscular volume were significantly reduced after 21 days of exposure to 0.05 mg·L−1 of DNZ (p < 0.05). The present study shows that DZN has various toxic effects on grass carp, which may pose a potential risk for other fish species.

1. Introduction

Today, residual concentrations of pesticides are ubiquitously found in all environmental compartments worldwide [1,2,3,4,5,6]. Due to the increasing usage of pesticides, these environmental pollutants directly or indirectly threaten aquatic ecosystems the most [1,7,8,9]. On the other hand, pesticides are needed for maintaining increasing efficiency of agricultural production due to growing populations [10,11,12,13]. The importance of aquatic ecosystems is priceless for humans, organisms, and even for the function of other compartments of nature [1,7,8,9].
The provinces of Guilan, Mazandaran, and Golestan, with a combined surface area of 1.5 million ha in the north of Iran, are dedicated to cultivating various standard and rain-fed crops [14,15,16,17]. According to the FAO database [18], more than 6.8 tons of pesticides were used in agricultural areas in 2019, with 1.7 tons of insecticides in Iran. There is growing global consumption of one of the most frequently used organophosphorus insecticides—diazinon (DZN), which is primarily used in agriculture, households, and recreational areas [19,20,21,22]. Generally, the highest concentration of DZN in catchments usually occurs after leaching from agricultural areas, followed by heavy rainfall and runoff into areas where DZN is not used [23]. Moreover, depending on their manufacturer or classification, commercial pesticides usually have a different percentage of the active ingredient. Commercial pesticides usually contain different solvents, which increase their dissolution, half-life, and toxicity compared with initial compositions [4]. The next issue may be the bioaccumulation index, which in the case of DZN varies in different fish species [24]. The most important abnormality recorded due to DZN toxicity is vertical and horizontal deformation of the spine [25]. The effects of DZN on blood indices in grass carp include decreased red blood cell (RBC) counts and drops in haematocrit (Hct) and haemoglobin (Hb) values [26].
All of these reasons together may lead to the problematic detection of source pollution of DZN and its monitoring. Despite these difficulties, Dahmardeh Behrooz et al. [27] detected diazinon in three major rivers of the Caspian Sea at levels of 41 to 145 ng·L−1. Notably, residual pesticide concentrations have more destructive effects on non-target aquatic organisms than on target organisms (pests) in several cases, resulting in higher susceptibility and faster and higher mortality [28,29,30,31,32]. DZN at a concentration of more than 10 mg·L−1 in water can cause acute toxic effects on freshwater ecosystems [33]. The toxicity of DZN is based on its ability to block acetylcholinesterase (AChE) activity, which influences the nervous systems of fish and, consequently, disturbs the secretion of the hormones [19,33]. Generally, organophosphorus pesticides in the fish lead to loss of physical strength, stiffness, and deformity of the spine in larvae and juveniles—possibly due to impaired muscle control [34].
Fish populations are one of the essential links in the food network, with humans at the top [19,33]. Considering the linking of trophic levels, pesticides are closely related to fish protein when absorbed through the gills, as the primary target organ, and pose a health risk when contaminated fish is eaten [35]. Therefore, fish may be considered good indicators of contamination, because of their biochemical responses [15,24,35,36,37,38,39]. Pesticides strongly influence fish’s vital organs in various ways. Environmental stress caused by xenobiotics usually affects fish and other aquatic organisms through reactive oxygen species mechanisms, leading to tissue damage (e.g., hyperplasia, cytoplasmic vacuolation, and necrosis), as well as changes in behavioural responses (e.g., changes in swimming patterns) and haematological parameters (e.g., haematocrit, red blood cell counts, and haemoglobin) [35,40,41,42,43,44,45,46].
The objective of this study was to evaluate the acute and chronic toxicity of the commercial insecticide diazinon (DZN, 60% active agent) to grass carp fingerlings (Ctenopharyngodon idella, Valenciennes 1844), which belong to the family Cyprinidae (Teleostei). The euryhaline grass carp is mainly native to East Asia’s freshwater rivers and lakes, tolerating a wide temperature range from 7 to 33 °C [47]. It is one of the most highly steamed fish species in the northern part of Iran. Histological changes in gills and livers were observed after an acute toxicity test (96 h LC50). Changes in red blood cell (RBC) and white blood cell (WBC) counts, haemoglobin concentration (Hb), haematocrit (Hct), mean corpuscular volume (MCV), and mean corpuscular haemoglobin concentration (MCHC) were detected after 21 days of chronic exposure.

2. Materials and Methods

2.1. Ethical Standard

The used procedures for rearing, toxicity, and sampling agreed with protocol 27140 recommended by the ethical committee of the Gorgan University of Agricultural Science and Natural Resources in 2019. Fish were acclimated with methods for experimental tests as suggested for fish, macroinvertebrates, and amphibians [41,42,43].

2.2. Testing Chemical

The commercial insecticide diazinon, with 60% active agent, was purchased from the company Alborz Behsam Co. in Tehran (Iran).

2.3. Experimental Animals

Fingerling grass carp (Ctenopharyngodon idella; N = 217, weight 14.4 ± 1.3 g) were purchased from a local commercial hatchery (Iran). Adaptation for laboratory conditions was maintained in 10 tanks, each with 250 L of tap water. During one week of acclimatisation, juveniles were fed twice per day at a rate of 2% body weight by commercial feed (GC-705, Faradane Co., Shahrekord, Iran) until 24 h before the initiation test [48]. The experimental room was maintained with a 12 h light and 12 h dark photoperiod and a temperature of 23 ± 2 °C. Water physicochemical parameters were measured daily (pH 7.4–7.9, NH3 < 0.02 mg·L−1, BOD 865 ± 45 mg·L−1, CaCO3 210 mg).

2.4. Acute Toxicity Test (LC50)

A preliminary acute toxicity test was conducted according to the procedure described in OECD guideline 203 [49]. In total, 147 grass carp fingerlings were randomly selected and divided into one control group and six treatment groups (21 fingerlings per group). Diazinon (DZN) concentrations were 0, 0.5, 0.75, 1.5, 2, 2.5, and 3 mg·L−1 during the acute toxicity test (LC50 96 h). The chemical was used as described by Chorehi et al. [16] in the laboratory facility. Each test was performed in triplicate with four time intervals (24, 48, 72, and 96 h). Experiments were carried out in 21 fiberglass tanks (dehydration volume: 50 L). Fish were not fed during the acute toxicity tests, and water was not replaced. The survival under different DZN concentrations was evaluated and counted over the exposure period, and the dead fish were removed immediately. Median lethal concentrations of DZN were calculated by probit analysis using SPSS software (version 19.0) (Armonk, NY, USA).

Histopathological Observation

Samples were taken from live fish after 96 h. They were dissected from the gills to the anus and the viscera. The gills and liver were fixed in Bouin’s fluid for 48 h and kept in a buffer solution for the next 12 h. After dehydration, the organs were submerged in paraffin. Slides at a thickness of 5–7 μm were produced using a microtome. After staining with hematoxylin and eosin (H&E), the slides were examined under a light microscope and photographed [23].

2.5. Chronic Toxicity Test

The chronic toxicity of diazinon (DZN) was determined according to acute toxicity test evaluation on grass carp fingerlings (N = 63). Fish were randomly divided into three groups (21 fish per group). Diazinon concentrations were 0, 0.01, and 0.05 mL·L−1. Each group of tests was performed in triplicate, and sublethal concentrations were tested for 21 days [2]. The nominal concentration of DZN was restored by computing daily water exchange in each tank (dehydration volume: 50 L) and adding the DZN. During the test, fish were fed twice a day at a rate of 2% body weight with commercial feed (GC-705, Faradane Co., Shahrekord, Iran). The photoperiod and water physicochemical parameters responded to the adaptation period.

Haematological Parameters

After 21 days, seven fish were randomly sampled from each treatment and killed quickly with a blow to the head. Blood was taken immediately from the caudal vessels using heparinised syringes and transferred to heparinised 1.5 mL Eppendorf tubes, kept on ice. The whole blood was suspended in the diluent for red and white blood cell levels using a haemocytometer [50]. Haemoglobin concentration (Hb) was measured using a commercial kit (Pars Azmun Co., Tehran, Iran) by photometric assay of cyanmethemoglobin. Furthermore, the chemical solutions were centrifuged before measuring the absorbance. Haematocrit (Hct) was determined by centrifuging whole blood in heparinised microhaematocrit capillary tubes at 3500× g for 10 min (Osterode, Germany). Mean corpuscular volume (MCV), mean red blood cell count (RBC), mean white blood cell count (WBC), and mean haemoglobin concentration (Hb) were calculated as described by Houston [51].

2.6. Data Analysis

Data analyses were performed using SPSS 19.0 software (IBM, SPSS Inc., Chicago, IL, USA). Before conducting statistical analyses, all data were tested for normality (Kolmogorov–Smirnov test). One-way analysis of variance (ANOVA) followed by Tukey’s post hoc test was used to assess the significant effects of DZN concentrations on mortality rate. All of the data were expressed as the mean ± standard deviation (SD). Median lethal concentrations of DZN for these fish were calculated by probit analysis. A value of p < 0.05 was considered statistically significant for all tests. The levels of tissue damage in the samples were scored as described by Banaee et al. [20] and Yalsuyi et al. [15].

3. Results

3.1. Acute Toxicity Test (LC50)

Mortality was not observed during the adaptation period in the control group (without DZN) or in any of the treatments during the LC50 96 h test. Fish’s physical symptoms due to DZN’s toxicity to grass carp species were clinically observed, including body tremor; haemorrhage in the caudal fin and head; irregular, rapid, and sudden circular swimming; and lethargy and discolouration of the studied fish. Results concerning the effects of DZN on grass carp fingerlings were observed at concentrations of 1.5 and 2 mg·L−1, with 48 and 72 h mortality rates over 50%, while at 2.5 and 3 mg·L−1, almost 100% loss was always observed. There was a significant correlation between DZN concentration and fish mortality rate (p < 0.01; Figure 1).
After 96 h, results showed that nominal DNZ concentrations higher than 0.099 mg·L−1 can lead to fish mortality; moreover, the 96 h LC50 of DZN was 1.124 mg·L−1 (Table 1).

Histopathological Observation

Tissue damage was not observed in the control group samples. There was a significant correlation between tissue damage and concentrations of DZN (p < 0.01). Several forms of liver damage—such as cytoplasmic vacuolation of the hepatocytes, necrosis of liver tissue, dilation of sinusoids, and vascular dilation—were observed in all treatments (Table 2). Damage to the liver at different doses of DZN is shown in Figure 2.
Gill lesions were observed in all treatments. There was a significant correlation between gill lesions and DZN concentration (p < 0.01). Lethal concentrations of DZN led to primary lamellar oedema, hyperplasia, and hypertrophy of squamous cells of the secondary lamellae. Secondary lamellar sloughing, haemorrhage, and necrosis of gill cells and tissue were also observed (Figure 3). However, primary lamellar oedema, hyperplasia, and hypertrophy of the gills were more commonly seen than other lesions (Table 3).

3.2. Chronic Toxicity Test

Some clinical signs—such as behavioural alterations, increased skin mucus, unbalanced swimming on the water surface, slow swimming near the water surface, increased opercular movements, and alterations in the fish’s colour patterns—were observed in fish exposed to sublethal concentrations of DZN.

Haematological Parameters

Haematocrit, haemoglobin, red blood cells, and white blood cells were significantly reduced after 21 days of exposure (Table 4). However, there were no significant differences between blood parameters in the control group and those treated with 0.01 mg·L−1 of DZN (p > 0.05). Finally, the mortality rate at 0.05 mg·L−1 of DNZ was less than 10% of the fish population.
As shown in Table 5, the coefficient of determination did not differ significantly during the experiments. The minimum exposure time was considered to be 24 h. Based on LC50, DZN can be classified as a toxic pesticide.

4. Discussion

Environmental pollution—especially with industrial wastewater containing various pollutants, toxic metals, and agricultural pesticides—is the most crucial factor in the morbidity and mortality of aquatic organisms—especially fish [53,54,55,56]. There are several methods for evaluating the toxicity of pollutants, such as blood assessments, physiological studies, or histopathological studies. However, each of these methods has several limitations. For example, the 96 h LC50 test can measure only lethal concentrations of contaminants in laboratory conditions. Hence, using two or three methods together can be a valuable tool for assessing toxicity to organisms. [57]. This assessment was proven by this study on the toxicity of DZN to grass carp (Ctenopharyngodon idella) fingerlings. The 96 h LC50 test was performed with an evaluation of gill and liver histology, followed by a chronic toxicity test of complementary changes in haematological parameters.
This study’s haematological assessments showed that sublethal concentrations of DZN can cause liver and gill lesions, and reduce haematocrit and blood cell counts in fish, as was made evident during the tests. A fish’s physiological response to the contaminated environment is reflected in haematological indices, such as haemoglobin, haematocrit, and blood cell counts, inducing alterations in fish’s oxygen-carrying capacity via reducing RBC counts and osmotic disturbances via tissue lesions. Significant changes occur in the liver structure due to DZN exposure, including necrosis and hypertrophy, decreasing the fish’s Hb, RBCs, WBCs, and Hct values. According to Table 2, a decrease was observed in RBC counts, haemoglobin, and haematocrit levels in the fish exposed to different concentrations of DZN compared to the control group. Fish exposed to pesticides are likely to be overactive so as to get out of a stressful environment, and they need more oxygen to increase their energy requirements [6]. Alternatively, fish may secrete mucus in large quantities to cover the body—especially the gills—to eliminate the toxin’s irritating effects. Undoubtedly, this coating reduces gas exchange through the gills. Due to the decrease in supply and elevated oxygen demand, oxygen deficiency is expected to occur in the fish [58]. The reduction in RBC counts depends on the damage to membrane structure and the accumulation of RBCs in the gills, stress resulting from malnutrition, the disintegration of RBCs, and the biological concentration of toxins in the kidneys and liver [51,59]. WBCs play an essential role in the body’s immune system. The reduced number of WBCs and, consequently, weakened immune system observed in the present study may reflect leucopoiesis degradation and direct damage to the cell membrane [53,60,61]. Al-Otaibi et al. [62] evaluated the chronic effects of DZN on the blood, liver, and gills of catfish (Clarias gariepinus), reporting that increased levels of DZN reduced the number of WBCs, similar to the present study. One of the reasons for the decrease in RBCs is the decrease in haemoglobin levels, and iron is one of the main components of haemoglobin in the blood stored in the liver [58]. One disadvantage of DZN we observed was the reduction in iron content due to the effect of this toxin on the liver, which reduces the levels of haemoglobin in the blood. With the decrease in haemoglobin levels, tissues are exposed to low oxygen levels, leading to decreased metabolism and physical activity [20]. DZN, as an organophosphate, is an acetylcholinesterase inhibitor, causing continuous stimulation of muscle and nerve fibres and, consequently, fatigue and tetanus [63]. This nerve stimulation can induce immune system alterations by affecting the transmission of signals from nerve terminals to cells [64,65].
Fish’s increasing or decreasing motility and behavioural patterns mainly interfere with nervous systems and sensory receptors, resulting from the fish’s responses to environmental stimuli, such as pesticides [20,66]. In the present study, several clinical signs of behavioural changes were recorded in fish, such as unbalanced swimming on the water surface, slow swimming near the water surface, and anxiety, which can constitute a direct effect of DZN. Al-Asgah et al. [67] linked behavioural changes and blood cell responses. They reported that alterations in these indices reveal toxic stress in the treated animals. Therefore, it is crucial to understand the destructive effects of pesticides on the fish as an essential link in the food chain, because changes in the food chain may lead to an imbalance in the whole aquatic ecosystem [67]. The present study’s results were similar to those of Pirbeigi et al. [11] and Kavitha et al. [1].
Insecticides may influence acetylcholinesterase activity, leading to decreased motility in the fish [54,55,59]. DZN has also shown to be metabolised into toxic derivatives in the liver via cytochrome P450 monooxygenase [68].
The effect of certain insecticides on acetylcholinesterase activity may also lead to decreasing motility in the fish. DZN was reported to be metabolised into toxic derivatives in the liver by cytochrome P450 monooxygenases, hydrolysed in microsomes, and then excreted from the body. In a study on the effects of DZN on the Persian sturgeon, (Acipenser persicus), the 96 h LC50 was reported as 4.38 mg·L−1 [69]. In the case of A. nudiventris and Rutilus kutum, it was 3.6 and 1.9 mg·L−1, respectively [70]. Measuring haematological indices is a good tool for evaluating the effects of pollutants on fish. Al-Asgah et al. [67] reported alterations in several haematological indices as indicators of exposure to pollutants. In the present study, the liver was exposed to 1, 1.5, 2, 2.5, and 3 mg·L−1 for 96 h. When elevating the DZN concentration, the damage to the liver increased. These lesions started through the aggregation of erythrocytes within hepatic blood vessels at 1 mg·L−1, and continued to extensive necrosis at 3 mg·L−1. In this study, the gills were also exposed to 1, 1.5, 2, 2.5, and 3 mg·L−1 for 96 h. The lesions started with hyperaemia at 1 mg·L−1, and continued to extensive necrosis of gill cells and haemorrhage at 3 mg·L−1 (Table 3).
Hedayati et al. [47] reported that deltamethrin causes more serious gill damage than DZN during chronic toxicity tests on the iridescent shark (Pangasius hypophthalmus). Other studies have reported changes in the morphology of the gills when exposed to toxins [21,71].
Gill damage leads to impaired gas exchange capacity and respiratory distress. Similar results concerning gill damage in fish after exposure to some toxins were reported by Omitoyin et al. [72], Rahman et al. [73], and Ezemony and Ogbomida [74]. Svoboda et al. [75] reported that DZN significantly affects blood indices in grass carp by reducing the number of red blood cells, as well as levels of haemoglobin and haematocrit. They suggested that these alterations after exposure to DZN could be due to the destruction of haematopoietic tissues in the fish’s kidneys. Although haematopoietic organs were not examined in the present study, the results related to blood parameters were similar to the findings of their study. These results reinforce the possibility that diazinon may have a toxic effect on hematopoietic tissues.

5. Conclusions

This study showed that diazinon (DZN) is classified as poisonous to grass carp (C. idella) and, even at low concentrations, can alter their haematological indices and histopathology. Histopathological parameters are sensitive and significant indicators for the evaluation of the effects of DZN on fish. Moreover, tissues such as the gills and liver can be used to monitor the effects of these toxins in fish over a short time. Further investigations are needed to better understand the harmful effects of DZN—especially with respect to the recovery of blood and other tissues over time. In general, the findings of this study provide information about the white blood cell counts and red blood cell counts in fish, and the effects of DZN on these indices, as well as on liver and gill tissues.

Author Contributions

Conceptualisation, S.V. and N.M.; methodology, D.A. and N.T.; software, M.V.; validation, C.F.; formal analysis, M.V. and N.T.; investigation, N.M.; resources, S.V.; data curation, C.F. and N.T.; writing—original draft preparation, S.V.; writing—review and editing, D.A., N.M. and N.T.; supervision, C.F.; project administration, S.V. and N.M. All authors have read and agreed to the published version of the manuscript.

Funding

This research received no external funding.

Institutional Review Board Statement

The protocol of the study was according to guidelines issued by the Gorgan University of Agricultural Sciences and Natural Resources Research Ethics Committee No. IR-GAUEC207s-2020.

Informed Consent Statement

Not applicable.

Data Availability Statement

The datasets in this study are available from the corresponding author upon reasonable request. All data and materials are available for publication.

Acknowledgments

The authors give special thanks to Marko D. Prokić and all of the people who helped them to complete this study.

Conflicts of Interest

The authors declare that they have no conflict of interest.

References

  1. Kavitha, C.; Malarvizhi, A.; Senthil Kumaran, S.; Ramesh, M. Toxicological effects of arsenate exposure on hematological, biochemical and liver transaminases activity in an Indian major carp, Catla catla. Food Chem. Toxicol. 2010, 48, 2848–2854. [Google Scholar] [CrossRef]
  2. Vajargah, M.F.; Hedayati, A.; Yalsuyi, A.M.; Abarghoei, S.; Gerami, M.H.; Farsani, H.G. Acute toxicity of Butachlor to Caspian Kutum (Rutilus frisii Kutum Kamensky, 1991). J. Environ. Treat. Tech. 2014, 2, 155–157. [Google Scholar]
  3. Vajargah, M.F.; Yalsuyi, A.M.; Hedayati, A. Effects of dietary Kemin multi-enzyme on survival rate of common carp (Cyprinus carpio) exposed to abamectin. Iran. J. Fish. 2018, 17, 564–572. [Google Scholar]
  4. Vajargah, M.F.; Yalsuyi, A.M.; Sattari, M.; Hedayati, A. Acute toxicity effect of glyphosate on survival rate of common carp, Cyprinus carpio. Environ. Health Eng. Manag. J. 2018, 5, 61–66. [Google Scholar] [CrossRef]
  5. Stara, A.; Pagano, M.; Albano, M.; Savoca, S.; Di Bella, G.; Albergamo, A.; Zuskova, E.; Sandova, M.; Velisek, J.; Fabrello, J.; et al. Effects of long-term exposure of Mytilus galloprovincialis to thiacloprid: A multibiomarker approach. Environ. Pollut. 2021, 289, 117892. [Google Scholar] [CrossRef]
  6. Shaluei, F.; Hedayati, A.; Jahanbakhshi, A.; Kolangi, H.; Fotovat, M. Effect of subacute exposure to silver nanoparticle on some hematological and plasma biochemical indices in silver carp (Hypophthalmichthys molitrix). Hum. Exp. Toxicol. 2013, 32, 1270–1277. [Google Scholar] [CrossRef]
  7. Sanchez-Fortun, S.; Barahona, M.V. Comparative study on the environmental risk induced by several pyrethroids in estuarine and freshwater invertebrate organisms. Chemosphere 2005, 59, 553–559. [Google Scholar] [CrossRef]
  8. Hedayati, A.; Tarkhani, R. Hematological and gill histopathological changes in iridescent shark, Pangasius hypophthalmus (Sauvage, 1878) exposed to sublethal diazinon and deltamethrin concentrations. Fish Physiol. Biochem. 2014, 40, 715–720. [Google Scholar] [CrossRef]
  9. Vajargah, M.F.; Hedayati, A. Acute toxicity of trichlorofon on four viviparous fish: Poecilia latipinna, Poecilia reticulata, Gambusia holbrooki and Xiphophorus helleri (Cyprinodontiformes: Poeciliidae). J. Coast. Life Med. 2014, 2, 511–514. [Google Scholar]
  10. Bibak, M.; Tahmasebi, S.; Sattari, M.; Kafaei, R.; Ramavandi, B. Empirical cumulative entropy as a new trace elements indicator to determine the relationship between algae-sediment pollution in the Persian Gulf, southern Iran. Environ. Sci. Pollut. Res. 2021, 28, 2634–2644. [Google Scholar] [CrossRef]
  11. Pirbeigi, A.; Poorbagher, H.; Eagderi, S.; Mirvaghefi, A.R. Pathological effects of sublethal diazinon on the blood, gill, liver and kidney of the freshwater fish Capoeta damascina. J. Chem. Ecol. 2016, 32, 270–285. [Google Scholar] [CrossRef]
  12. Stara, A.; Bellinvia, R.; Velisek, J.; Strouhova, A.; Kouba, A.; Faggio, C. Acute exposure of common yabby (Cherax destructor) to neonicotinoid pesticide. Sci. Total Environ. 2019, 665, 718–723. [Google Scholar] [CrossRef]
  13. Stara, A.; Kubec, J.; Zuskova, E.; Buric, M.; Faggio, C.; Kouba, A.; Velisek, J. Effects of S-metolachlor and its degradation product metolachlor OA on marbled crayfish (Procambarus virginalis). Chemospere 2019, 224, 616–625. [Google Scholar] [CrossRef]
  14. Vajargah, M.F.; Sattari, M.; Namin, J.I.; Bibak, M. Predicting the Trace Element Levels in Caspian Kutum (Rutilus kutum) from south of the Caspian Sea Based on Locality, Season and Fish Tissue. Biol. Trace Elem. Res. 2021, 2021, 354–363. [Google Scholar]
  15. Lavanya, S.; Ramesh, M.; Kavitha, C.; Malarvizhi, A. Hematological, biochemical and ionoregulatory responses of Indian major carp Catla catla during chronic sublethal exposure to inorganic arsenic. Chemosphere 2011, 82, 977–985. [Google Scholar] [CrossRef]
  16. Chorehi, M.M.; Ghaffari, H.; Hossaini, S.A.; Niazie, E.H.N.; Vajargah, M.F.; Hedayati, A. Acute toxicity of Diazinon to the Caspian vimba, Vimba vimba persa (Cypriniformes: Cyprinidae). Int. J. Aquat. Biol. 2013, 1, 254–257. [Google Scholar]
  17. Vajargah, M.F.; Hossaini, S.A.; Niazie, E.H.N.; Hedayati, A.; Vesaghi, M.J. Acute toxicity of two pesticides Diazinon and Deltamethrin on Tench (Tinca tinca) larvae and fingerling. Int. J. Aquat. Biol. 2013, 1, 138–142. [Google Scholar]
  18. FAO; WHO. General Standard for Contaminants and Toxins in Food and Feed (Codex Stan 193-1995). Available online: https://www.fao.org/fao-who-codexalimentarius/sh-proxy/en/?lnk=1&url=https%253A%252F%252Fworkspace.fao.org%252Fsites%252Fcodex%252FStandards%252FCXS%2B193-1995%252FCXS_193e.pdf (accessed on 12 March 2022).
  19. Larkin, D.J.; Tjeerdema, R.S. Fate and effects of Diazinon. Rev. Environ. Contam. Toxicol. 2000, 166, 49–82. [Google Scholar]
  20. Banaee, M.; Sureda, A.; Mirvaghefi, A.R.; Ahmadi, K. Biochemical and histological changes in the liver tissue of rainbow trout (Oncorhynchus mykiss) exposed to sublethal concentrations of Diazinon. Fish Physiol. Biochem. 2013, 39, 489–501. [Google Scholar] [CrossRef]
  21. Dutta, H.M.; Munshi, J.S.D.; Roy, P.K.; Singh, N.K.; Motz, L.; Adhikari, S. Effects of diazinon on blue gill sunfish, Lepomis macrochirus, gills: Scanning electron microscope observations. Exp. Biol. Online 1997, 2, 1–11. [Google Scholar] [CrossRef]
  22. Saha, S.; Chukwuka, A.V.; Mukherjee, D.; Patnaik, L.; Nayak, S.; Dhara, K.; Saha, N.C.; Faggio, C. Chronic effects of Diazinon® exposures using integrated biomarker responses in freshwater walking catfish, Clarias batrachus. Appl. Sci. 2021, 11, 10902. [Google Scholar] [CrossRef]
  23. Li, Z.H.; Zlabek, V.; Velíšek, J.; Grabic, R.; Machova, J.; Kolařová, J.; Li, P.; Randák, T. Antioxidant responses and plasma biochemical characteristics in the freshwater rainbow trout, Oncorhynchus mykiss, after acute exposure to the fungicide propiconazole. Czech J. Anim. Sci. 2011, 56, 61–69. [Google Scholar] [CrossRef]
  24. Dar, O.I.; Aslam, R.; Pan, D.; Sharma, S.; Andotra, M.; Kaur, A.; Jia, A.; Faggio, C. Source, bioaccumulation, degradability and toxicity of triclosan in aquatic environments: A review. Environ. Technol. Innov. 2021, 25, 102122. [Google Scholar] [CrossRef]
  25. Debski, B.; Kania, B.F.; Kuryl, T. Transformations of diazinon, an organophosphate compound in the environment and poisoning by this compound. Ecology-Bratislava 2007, 26, 68. [Google Scholar]
  26. Ullah, S.; Zorriehzahra, M.J. Ecotoxicology: A review of pesticides induced toxicity in fish. Adv. Anim. Vet. Sci. 2015, 3, 40–57. [Google Scholar] [CrossRef]
  27. Dahmardeh Behrooz, R.; Esmaili-sari, A.; Urbaniak, M.; Chakraborty, P. Assessing Diazinon Pollution in the Three Major Rivers Flowing into the Caspian Sea (Iran). Water 2021, 13, 335. [Google Scholar] [CrossRef]
  28. Pagano, M.; Stara, A.; Aliko, V.; Faggio, C. Impact of Neonicotinoids to Aquatic Invertebrates—In Vitro Studies on Mytilus galloprovincialis: A Review. J. Mar. Sci. Eng. 2020, 8, 801. [Google Scholar] [CrossRef]
  29. Stara, A.; Pagano, M.; Capillo, G.; Fabrello, J.; Sandova, M.; Albano, M.; Zuskova, E.; Velisek, J.; Matozzo, V.; Faggio, C. Acute effects of neonicotinoid insecticides on Mytilus galloprovincialis: A case study with the active compound thiacloprid and the commercial formulation Calypso 480 S. Ecotoxicol. Environ. Saf. 2020, 203, 110980. [Google Scholar] [CrossRef]
  30. Stara, A.; Pagano, M.; Capillo, G.; Fabrello, J.; Sandova, M.; Vazzana, I.; Zuskova, E.; Velisek, J.; Matozzo, V.; Faggio, C. Assessing the effects of neonicotinoid insecticide on the bivalve mollusc Mytilus galloprovincialis. Sci. Total Environ. 2020, 700, 134914. [Google Scholar] [CrossRef]
  31. Sula, E.; Aliko, V.; Barceló, D.; Faggio, C. Combined effects of moderate hypoxia, pesticide and PCBs upon crucian carp fish, Carassius carrasius, from a freshwater lake-in situ ecophysiological approach. Aquat. Toxicol. 2020, 228, 105644. [Google Scholar] [CrossRef]
  32. Khara, H.; Salar Amoli, J.; Mazloumi, H.; Nezami, S.H.A.; Zolfinezhad, K.; Khodaparast, S.H.; Hasan, J.; Akbarzadeh, A.; Mohammadi, S.; Gholipour, S.; et al. Survey and seasonal measurement of pesticide (hinosan, machete and diazinon) in water of Oshmak River (east of Guilan). J. Biol. Sci. 2008, 2, 29–43. [Google Scholar]
  33. Yalsuyi, A.M.; Vajargah, M.F. Recent advance on aspect of fisheries: A review. J. Coast. Life Med. 2017, 5, 141–148. [Google Scholar] [CrossRef]
  34. Montajami, S.; Hajiahmadyan, M.; Forouhar Vajargah, M.; Hosseini Zarandeh, A.S.; Shirood Mirzaie, F.; Hosseini, S.A. Effect of symbiotic (Biomin imbo) on growth performance and survival rate of Texas cichlid (Herichthys cyanoguttatus) larvae. Glob. Vet. 2012, 9, 358–361. [Google Scholar]
  35. Lackner, R. Oxidative stress in fish by environmental pollutants. In Fish Ecotoxicology; Birkhäuser: Basel, Switzerland, 1998; pp. 203–224. [Google Scholar]
  36. Paduraru, E.; Flocea, E.I.; Lazado, C.; Simionov, I.A.; Nicoara, M.; Ciobica, A.; Faggio, C.; Jijie, R. Vitamin C mitigates oxidative stress and behavioral impairments induced by deltamethrin and lead toxicity in zebrafish. Int. J. Mol. Sci. 2021, 22, 12714. [Google Scholar] [CrossRef] [PubMed]
  37. Sharma, S.; Dar, O.I.; Andotra, M.; Sharma, S.; Kaur, A.; Faggio, C. Environmentally relevant concentrations of Triclosan induce cyto-genotoxicity and biochemical alterations in the hatchlings of Labeo rohita. Appl. Sci. 2021, 11, 10478. [Google Scholar] [CrossRef]
  38. Blahova, J.; Cocilovo, C.; Lucie Plhalova, L.; Svobodova, Z.; Faggio, C. Embryotoxicity of atrazine and its degradation products to early life stages of zebrafish (Danio rerio). Environ. Toxicol. Pharmacol. 2020, 77, 103370. [Google Scholar] [CrossRef]
  39. Mohamadi Yalsuyi, A.; Forouhar Vajargah, M.; Hajimoradloo, A.; Mohammadi Galangash, M.; Prokić, M.D.; Faggio, C. Can Betadine (10% povidone-iodine solution) act on the survival rate and gill tissue structure of Oranda goldfish (Carassius auratus)? Vet. Res. Commun. 2021, 2021, 1–8. [Google Scholar] [CrossRef]
  40. Forouhar Vajargah, M.; Imanpour, M.R.; Shabani, A.; Hedayati, A.; Faggio, C. Effect of long-term exposure of silver nanoparticles on growth indices, hematological and biochemical parameters and gonad histology of male goldfish (Carassius auratus gibelio). Microsc. Res. Tech. 2019, 82, 1224–1230. [Google Scholar] [CrossRef]
  41. Faria, M.; Prats, E.; Ramírez, J.R.R.; Bellot, M.; Pagano, M.; Valls, A.; Gomez-canela, C.; Porta, J.M.; Mestres, J.; Garcia-reyero, N.; et al. Androgenic activation, impairment of the monoaminergic system and altered behavior in zebrafish larvae exposed to environmental concentrations of fenitrothion. Sci. Total Environ. 2021, 775, 145671. [Google Scholar] [CrossRef]
  42. Strungaru, S.A.; Pohontiu, C.M.; Nicoara, M.; Teodosiu, C.; Baltag, E.S.; Jijie, R.; Plavan, G.; Pacioglug, O.; Faggio, C. Response of aquatic macroinvertebrates communities to multiple anthropogenic stressors in a lowland tributary river. Environ. Toxicol. Pharmacol. 2021, 16, 103687. [Google Scholar] [CrossRef]
  43. Jijie, R.; Mihalache, G.; Balmus, I.M.; Strungaru, S.A.; Baltag, E.S.; Ciobica, A.; Nicoara, M.; Faggio, C. Zebrafish as a screening model to study the single and joint effects of antibiotics. Pharmaceuticals 2021, 14, 578. [Google Scholar] [CrossRef] [PubMed]
  44. Sharma, R.; Jindal, R.; Faggio, C. Impact of cypermethrin in nephrocytes of freshwater fish Catla catla. Environ. Toxicol. Pharmacol. 2021, 88, 103739. [Google Scholar] [CrossRef] [PubMed]
  45. Bussons, I.N.B.; Da Silva Souza, E.; Aride, P.H.R.; Paxiúba Duncan, W.L.; Pantoja-lima, J.; Furuyam, W.; Oliveira, A.T.; Bussons, M.R.F.M.; Faggio, C. Growth performance and hematological responses of Colossoma macropomum (Cuvier, 1818) fed graded levels of glycerol. Comp. Biochem. Physiol. Part C 2021, 249, 109122. [Google Scholar] [CrossRef] [PubMed]
  46. Stoliar, O.B.; Lushchak, V. Environmental Pollution and Oxidative Stress in Fish. In Oxidative Stress—Environmental Induction and Dietary Antioxidants; Lushchak, V.I., Ed.; IntechOpen: London, UK, 2012; pp. 131–166. [Google Scholar] [CrossRef] [Green Version]
  47. Hedayati, A.; Vajargah, M.F.; Yalsuyi, A.M.; Abarghoei, S.; Hajiahmadyan, M. Acute toxicity test of pesticide abamectin on common carp (Cyprinus carpio). J. Coast. Life Med. 2014, 2, 841–844. [Google Scholar]
  48. Sattari, M.; Namin, J.I.; Bibak, M.; Vajargah, M.F.; Hedayati, A.; Khosravi, A.; Mazareiy, M.H. Morphological comparison of western and eastern populations of Caspian kutum, Rutilus kutum (Kamensky, 1901) (Cyprinidae) in the southern Caspian Sea. Int. J. Aqua. Biol. 2019, 2, 242–247. [Google Scholar]
  49. OECD. Test No. 203: Fish, Acute Toxicity Test. In OECD Guidelines for the Testing of Chemicals; Organization for Economic Cooperation and Development: Paris, France, 1992. [Google Scholar]
  50. Natt, M.P.; Herrick, C.A. A new blood diluent for counting the erythrocytes and leucocytes of the chicken. Poult. Sci. 1952, 31, 735–738. [Google Scholar] [CrossRef]
  51. Houston, A.H. Blood and Circulation. In Methods in Fish Biology; Schreck, C.B., Moyle, P.B., Eds.; American Fisheries Society: Bethesda, MD, USA, 1990; 335p. [Google Scholar]
  52. U.S. EPA. Technical Overview of Ecological Risk Assessment—Analysis Phase: Ecological Effects Characterization: Ecotoxicity Categories for Terrestrial and Aquatic Organisms; United States Environmental Protection Agency: Washington, DC, USA, 2017. Available online: https://www.epa.gov/pesticide-science-and-assessing-pesticide-risks/technical-overview-ecological-risk-assessment-0 (accessed on 12 March 2022).
  53. Gallagher, E.P.; Digiulio, R.T. A comparison of glutathione-dependent enzymes in liver, gills and posterior kidney of channel catfish (Ictalurus punctatus). Comp. Biochem. Physiol. 1992, 102, 543–547. [Google Scholar] [CrossRef]
  54. Petrovici, A.; Strungaru, S.A.; Nicoara, M.; Robea, M.A.; Solcan, C.; Faggio, C. Toxicity of Deltamethrin to Zebrafish gonads revealed by cellular biomarkers. Mar. Sci. Eng. 2020, 8, 73. [Google Scholar] [CrossRef] [Green Version]
  55. Plhalova, L.; Blahova, J.; Divisova, L.; Enevova, V.; Casuscelli Di Tocco, F.; Faggio, C.; Tichy, F.; Vecerek, V.; Svobodova, Z. The effects of subchronic exposure to NeemAzal T/S on Zebrafish (Danio rerio). J. Chem. Ecol. 2018, 34, 199–210. [Google Scholar] [CrossRef]
  56. Blahova, J.; Doubkova, V.; Plhalova, L.; Lakdawala, P.; Medkova, D.; Vecerek, V.; Svobodova, Z.; Faggio, C. Embryotoxicity of selective serotonin reuptake inhibitors—Comparative sensitivity of zebrafish (Danio rerio) and African clawed frog (Xenopus laevis) embryos. Appl. Sci. 2021, 11, 10015. [Google Scholar] [CrossRef]
  57. Vijayaraghavan, K.; Balasubramanian, R. Is biosorption suitable for decontamination of metal-bearing wastewaters? A critical review on the state-of-the-art of biosorption processes and future directions. J. Environ. Manag. 2015, 160, 283–296. [Google Scholar] [CrossRef] [PubMed]
  58. Vajargah, M.F.; Hedayati, A. Toxicity effects of cadmium in grass carp (Ctenopharyngodon idella) and big head carp (Hypophthalmichthys nobilis). Transylv. Rev. Syst. Ecol. Res. 2017, 19, 43–48. [Google Scholar]
  59. Bartoskova, M.; Dobsikova, R.; Stancova, V.; Zivna, D.; Blahova, J.; Marsalek, P.; Zelníckova, L.; Bartos, M.; Di Tocco, F.C.; Faggio, C. Evaluation of ibuprofen toxicity for Zebrafish (Danio rerio) targeting on selected biomarkers of oxidative stress. Neuro Endocrinol. Lett. 2013, 34, 102–108. [Google Scholar] [PubMed]
  60. Al-Otaibi, A.M.; Al-Balawi, H.F.A.; Ahmad, Z.; Suliman, E.M. Toxicity bioassay and sub-lethal effects of diazinon on blood profile and histology of liver, gills and kidney of catfish, Clarias gariepinus. Braz. J. Biol. 2019, 79, 326–336. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  61. de Menezes, C.C.; Loro, V.L.; da Fonseca, M.B.; Cattaneo, R.; Pretto, A.; Miron, D.S.; Santi, A. Oxidative parameters of Rhamdia quelen in response to commercial herbicide containing clomazone and recovery pattern. Pestic. Biochem. Physiol. 2011, 100, 145–150. [Google Scholar] [CrossRef]
  62. Vajargah, M.F.; Hashemi, G.; Bibak, M.; Yalsuyi, A.M. The Effect of Vitamin C-Fortified Artemia on Growth and Survival of Sepia pharaonis Larvae. J. Environ. Treat. Tech. 2021, 9, 815–818. [Google Scholar]
  63. Fukuto, T.R. Mechanism of action of organophosphorus and carbamate insecticides. Environ. Health Perspect. 1990, 87, 245–254. [Google Scholar] [CrossRef]
  64. Sattari, M.; Bibak, M.; Vajargah, M.F. Trace and Macro Element Contaminations in Tissues of Vimba persa and Alosa braschnikowi From the South Caspian Sea and Potential Human Health Risk Assessment. Avicenna J. Environ. Health Eng. 2021, 8, 84–96. [Google Scholar] [CrossRef]
  65. Sattari, M.; Bibak, M.; Bakhshalizadeh, S.; Forouhar Vajargah, M. Element accumulations in liver and kidney tissues of some bony fish species in the Southwest Caspian Sea. J. Cell Mol. Res. 2020, 12, 33–40. [Google Scholar]
  66. Laetz, C.A.; Baldwin, D.H.; Collier, T.K.; Hebert, V.; Stark, J.D.; Scholz, N.L. The synergistic toxicity of pesticide mixtures: Implications for risk assessment and the conservation of endangered Pacific salmon. Environ. Health Perspect. 2009, 117, 348–353. [Google Scholar] [CrossRef] [Green Version]
  67. AL-Asgah, N.A.; Abdel-Warith, A.W.A.; Younis, E.M.; Allam, H.Y. Hematological and biochemical parameters and tissue accumulations of cadmium in Oreochromis niloticus exposed to various concentrations of cadmium chloride. Saudi J. Biol. Sci. 2015, 22, 543–550. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  68. Kretschmann, A.; Ashauer, R.; Preuss, T.G.; Spaak, P.; Escher, B.I.; Hollender, J. Toxicokinetic model describing bioconcentration and biotransformation of diazinon in Daphnia magna. Environ. Sci. Technol. 2011, 45, 4995–5002. [Google Scholar] [CrossRef] [PubMed]
  69. Rostami, H.; Soltani, M. The effect of diazinon toxin on hematologic indices of Acipenser nudiventris and its LC50 determination. Iran. Sci. Fish. J. 2005, 14, 49–60. [Google Scholar]
  70. Sheikhi, M.; Hajimoradloo, A.M.; Ghorbani, R.; Mollaei, M.; Khodanazary, A. Effects of Diazinon concentrations on LC50, hematocrit and clinical signs of Roach Torkemani (Rutilus rutilus caspius) fries of Caspian Sea. Iran. Sci. Fish. J. 2011, 20, 55–62. [Google Scholar]
  71. Yildirim, M.Z.; Benli, A.; Selvi, M.; Özkul, A.; Erkoc, F.; Koçak, O. Acute toxicity, behavioral changes, and histopathological effects of deltamethrin on tissues (gills, liver, brain, spleen, kidney, muscle, skin) of Nile tilapia (Oreochromis niloticus L.) fingerlings. Environ. Toxicol. 2006, 21, 614–620. [Google Scholar] [CrossRef] [PubMed]
  72. Omitoyin, B.O.; Ajani, E.K.; Adesina, B.T.; Okuagu, C.N.F. Toxicity of Lindane (Gamma Hexachloro-Cyclohexane) to Clarias gariepinus (Burchell 1822). World J. Zool. 2006, 1, 57–63. [Google Scholar]
  73. Rahman, M.Z.; Hossain, Z.; Mellah, M.F.R.; Ahmed, G.U. Effect of Diazinon 60 EC on Anabus testudinus, Channa punctatus and Barbades gomonotus. Naga ICLARM Q. 2002, 25, 8–11. [Google Scholar]
  74. Ezemonye, L.; Ogbomida, T.E. Histopathological effects of Gammalin 20 on African catfish (Clarias gariepinus). Appl. Environ. Soil Sci. 2010, 2010, 138019. [Google Scholar] [CrossRef] [Green Version]
  75. Svoboda, M.; Luskova, V.; Drastichova, J.; Zlabek, V. The effect of diazinon on haematological indices of common carp (Cyprinus carpio L.). Acta Vet. Brunesis-VFU Brno 2001, 70, 457–465. [Google Scholar] [CrossRef] [Green Version]
Water 14 01357 g001 550
Figure 1. Correlation between mortality rates of grass carp (Ctenopharyngodon idella) and diazinon (DZN) concentrations after 96 h.
Figure 1. Correlation between mortality rates of grass carp (Ctenopharyngodon idella) and diazinon (DZN) concentrations after 96 h.
Water 14 01357 g001
Water 14 01357 g002 550
Figure 2. The tissue damage in grass carp (Ctenopharyngodon Idella) fingerlings exposed to DZN: (a) Liver exposed to 1 mg·L−1 after 96 h (H&E, 10×), displaying cytoplasmic vacuolation (CV), vascular dilation (D), and the aggregation of erythrocytes within hepatic blood vessels (*). (b) Exposure to 1.5 mg·L−1 after 96 h (H&E, 10×), revealing cytoplasmic vacuolation (CV), necrosis (N), congestion of erythrocytes (yellow line), and dilation of sinusoids (DSS). (c) Exposure to 1.5 mg·L−1 after 96 h (H&E, 10×), indicating cytoplasmic vacuolation (CV), dilation of sinusoids (DSS), and the aggregation of erythrocytes within hepatic blood vessels (*). (d) Exposure to 2 mg L−1 after 96 h (H&E, 40×), exhibiting cytoplasmic vacuolation (CV) and necrosis (N). (e) Exposure to 2.5 mg·L−1 after 96 h (H&E, 40×), illustrating necrosis (N) and hyperaemia (H). (f) Exposure to 3 mg·L−1 after 96 h (H&E, 40×), depicting the extensive necrosis of the liver.
Figure 2. The tissue damage in grass carp (Ctenopharyngodon Idella) fingerlings exposed to DZN: (a) Liver exposed to 1 mg·L−1 after 96 h (H&E, 10×), displaying cytoplasmic vacuolation (CV), vascular dilation (D), and the aggregation of erythrocytes within hepatic blood vessels (*). (b) Exposure to 1.5 mg·L−1 after 96 h (H&E, 10×), revealing cytoplasmic vacuolation (CV), necrosis (N), congestion of erythrocytes (yellow line), and dilation of sinusoids (DSS). (c) Exposure to 1.5 mg·L−1 after 96 h (H&E, 10×), indicating cytoplasmic vacuolation (CV), dilation of sinusoids (DSS), and the aggregation of erythrocytes within hepatic blood vessels (*). (d) Exposure to 2 mg L−1 after 96 h (H&E, 40×), exhibiting cytoplasmic vacuolation (CV) and necrosis (N). (e) Exposure to 2.5 mg·L−1 after 96 h (H&E, 40×), illustrating necrosis (N) and hyperaemia (H). (f) Exposure to 3 mg·L−1 after 96 h (H&E, 40×), depicting the extensive necrosis of the liver.
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Water 14 01357 g003 550
Figure 3. The different tissue damage to the gills of fish exposed to different concentrations of DZN: (a) Fish gill lamellae exposed to 1 mg·L−1 DZN after 96 h (H&E, 40×), exhibiting primary lamellar oedema (yellow arrow). (b) Exposure to 1.5 mg·L−1 DZN after 96 h (H&E, 40×), displaying hyperplasia of secondary lamellae (yellow arrow). (c) Exposure to 1.5 mg·L−1 DZN after 96 h (H&E, 10×), indicating hypertrophy (HT) and primary lamellar oedema (*). (d) Exposure to 2 mg·L−1 DZN after 96 h (H&E, 10×), revealing haemorrhage (HR) and secondary lamellar shortening (N). (e) Exposure to 2.5 mg·L−1 DZN after 96 h (H&E, 10×), depicting secondary lamellar sloughing (SLA) and hypertrophy (HT). (f) Exposure to 3 mg·L−1 DZN after 96 h (H&E, 10×), illustrating necrosis (white arrow) and haemorrhage (HR).
Figure 3. The different tissue damage to the gills of fish exposed to different concentrations of DZN: (a) Fish gill lamellae exposed to 1 mg·L−1 DZN after 96 h (H&E, 40×), exhibiting primary lamellar oedema (yellow arrow). (b) Exposure to 1.5 mg·L−1 DZN after 96 h (H&E, 40×), displaying hyperplasia of secondary lamellae (yellow arrow). (c) Exposure to 1.5 mg·L−1 DZN after 96 h (H&E, 10×), indicating hypertrophy (HT) and primary lamellar oedema (*). (d) Exposure to 2 mg·L−1 DZN after 96 h (H&E, 10×), revealing haemorrhage (HR) and secondary lamellar shortening (N). (e) Exposure to 2.5 mg·L−1 DZN after 96 h (H&E, 10×), depicting secondary lamellar sloughing (SLA) and hypertrophy (HT). (f) Exposure to 3 mg·L−1 DZN after 96 h (H&E, 10×), illustrating necrosis (white arrow) and haemorrhage (HR).
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Table
Table 1. Lethal concentration (LC50) of diazinon (DZN) for grass carp fingerlings (Ctenopharyngodon idella).
Table 1. Lethal concentration (LC50) of diazinon (DZN) for grass carp fingerlings (Ctenopharyngodon idella).
PointConcentration (mg·L−1)
24 h48 h72 h96 h
LC100.8850.5210.3470.099
LC201.2110.8820.6940.451
LC301.4461.1420.9430.704
LC401.6471.3651.1570.921
LC501.8351.5731.3561.124
LC602.0221.7801.5561.326
LC702.2232.0031.7691.543
LC802.4582.2632.0191.796
LC902.7842.6242.3652.148
LC953.0532.9222.6512.438
Note: all concentrations of DZN were nominal concentrations.
Table
Table 2. Liver lesions in premature grass carp (Ctenopharyngodon idella) after exposure to six different concentrations of diazinon (DZN).
Table 2. Liver lesions in premature grass carp (Ctenopharyngodon idella) after exposure to six different concentrations of diazinon (DZN).
Liver Tissue DamageConcentration of DZN (mg·L−1)
00.511.522.53
Cytoplasmic vacuolation of the hepatocytes (CV)−+++++++++++++++++
Necrosis (N)−−++++++++++++++++
Dilation of sinusoids (DSS)−−+++++++++++++++++
Vascular dilation (D)−+++++++++++++++++++
Note: (−) no damage could be seen; (++) damage from 1 to 5; (+++) damage from 5 to 10; (++++) damage of more than 10; these scores were determined as described by Banaee et al. [20].
Table
Table 3. Gill histopathology in grass carp (Ctenopharyngodon idella) after exposure to different concentrations of diazinon (DZN).
Table 3. Gill histopathology in grass carp (Ctenopharyngodon idella) after exposure to different concentrations of diazinon (DZN).
Tissue DamagesConcentration (mg·L−1)
00.511.522.53
Primary lamellar oedema −+++++++++++++++++
Hyperplasia of epithelial cells−++++++++++++++++
Hypertrophy of epithelial cells−++++++++++++++++
Secondary lamellar sloughing−−++++++++++++++
Haemorrhage−−++++++++++++++
Necrosis−−−++++++++++++
(−) No gill tissue damage could be seen; (+) gill tissue damage from 1 to 3; (++) gill tissue damage from 3 to 5; (+++) gill tissue damage from 5 to 9; (++++) gill tissue damage from 9 to 15; these scores were determined as described by Yalsuyi et al. [15].
Table
Table 4. Blood parameters of grass carp (Ctenopharyngodon idella) after 21 days of exposure to sublethal concentrations of diazinon (DZN).
Table 4. Blood parameters of grass carp (Ctenopharyngodon idella) after 21 days of exposure to sublethal concentrations of diazinon (DZN).
Blood ParametersTreatments
Control0.01 *0.05 *
Haematocrit (%)26.73 ± 0.36 a26.66 ± 0.39 a19.73 ± 0.14 b
Haemoglobin (g/dL)8.04 ± 0.31 a7.91 ± 0.76 a5.25 ± 0.46 b
Red blood cells (106/μL)1.87 ± 0.07 a1.85 ± 0.11 a1.34 ± 0.4 b
White blood cells (103/μL)14,833.33 ± 828 a13,837 ± 782 a6000 ± 569 b
Mean corpuscular volume (fL)149.25 ± 1.23 a147.91 ± 1.63 a124.17 ± 0.87 b
* Nominal concentration of diazinon (DZN) mL·L−1. Different letters (a,b,c) indicate significant differences between values on the same row (p < 0.05).
Table
Table 5. Toxicity ratings of pesticides in living organisms according to the U.S. EPA [52].
Table 5. Toxicity ratings of pesticides in living organisms according to the U.S. EPA [52].
Toxicity Rating of Pesticides for Living OrganismsLC50 (mg·L−1)
Relatively non-toxic0 > 500
Less toxic100–500
Moderate toxicity10–100
Toxic1–10
Very toxic0.1–1
Highly toxic<0.1
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Vali, S.; Majidiyan, N.; Azadikhah, D.; Varcheh, M.; Tresnakova, N.; Faggio, C. Effects of Diazinon on the Survival, Blood Parameters, Gills, and Liver of Grass Carp (Ctenopharyngodon idella Valenciennes, 1844; Teleostei: Cyprinidae). Water 2022, 14, 1357. https://doi.org/10.3390/w14091357

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Vali S, Majidiyan N, Azadikhah D, Varcheh M, Tresnakova N, Faggio C. Effects of Diazinon on the Survival, Blood Parameters, Gills, and Liver of Grass Carp (Ctenopharyngodon idella Valenciennes, 1844; Teleostei: Cyprinidae). Water. 2022; 14(9):1357. https://doi.org/10.3390/w14091357

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Vali, Sara, Nava Majidiyan, Dariush Azadikhah, Matin Varcheh, Nikola Tresnakova, and Caterina Faggio. 2022. "Effects of Diazinon on the Survival, Blood Parameters, Gills, and Liver of Grass Carp (Ctenopharyngodon idella Valenciennes, 1844; Teleostei: Cyprinidae)" Water 14, no. 9: 1357. https://doi.org/10.3390/w14091357

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