Identification of Antibiotic Resistance Gene Hosts in Treatment Wetlands Using a Single-Cell Based High-Throughput Approach
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Site and Sampling Regime
2.2. epicPCR
2.3. Illumina Sequencing and Data Analysis
3. Results and Discussion
3.1. AMR Gene Hosts along the Watercourse of the Treatment Wetlands
3.2. Potential Class 1 Integron Carriers in the Treatment Wetlands
3.3. Evidence for AMR Genes in Methanogenic Archaea
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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intI1 | sul1 | sul2 | dfrA1 |
---|---|---|---|
Acidovorax | |||
Acinetobacter | Acinetobacter | Acinetobacter | |
Aeromonas | Aeromonas | Aeromonas | |
Bdellovibrio | |||
Citrobacter | |||
Commamonas | |||
Coxiella | Coxiella | ||
Dechloromonas | |||
Escherichia/Shigella | |||
Laribacter | |||
Legionella | |||
Paucibacter | |||
Protocatella | |||
Ralstonia | Ralstonia | Ralstonia | |
Simplicispira | Simplicispira | Simplicispira | |
Subdoligranulum | Subdoligranulum | ||
Thauera | |||
Thiovirga | Thiovirga | Thiovirga | |
Trichlorobacter | Trichlorobacter |
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Knecht, C.A.; Hinkel, M.; Mäusezahl, I.; Kaster, A.-K.; Nivala, J.; Müller, J.A. Identification of Antibiotic Resistance Gene Hosts in Treatment Wetlands Using a Single-Cell Based High-Throughput Approach. Water 2023, 15, 2432. https://doi.org/10.3390/w15132432
Knecht CA, Hinkel M, Mäusezahl I, Kaster A-K, Nivala J, Müller JA. Identification of Antibiotic Resistance Gene Hosts in Treatment Wetlands Using a Single-Cell Based High-Throughput Approach. Water. 2023; 15(13):2432. https://doi.org/10.3390/w15132432
Chicago/Turabian StyleKnecht, Camila A., Maja Hinkel, Ines Mäusezahl, Anne-Kristin Kaster, Jaime Nivala, and Jochen A. Müller. 2023. "Identification of Antibiotic Resistance Gene Hosts in Treatment Wetlands Using a Single-Cell Based High-Throughput Approach" Water 15, no. 13: 2432. https://doi.org/10.3390/w15132432