Effective Treatment of Acid Mine Drainage with Microbial Fuel Cells: An Emphasis on Typical Energy Substrates
Abstract
:1. Introduction
2. Materials and Methods
2.1. The Configuration of Microbial Fuel Cell (MFC) Reactors
2.2. Startup and Operation of MFC
2.3. Analysis and Calculations
2.4. Genomic DNA Extraction and MiSeq Sequencing of Bioelectroactive Biofilms
3. Results and Discussion
3.1. Effect of Different Energy Substrates on Single Chamber MFC Performance
3.2. Microbial Community of Anodic Bioelectroactive Biofilms
3.3. Contribution of Electroactive Biofilms on Anolyte’s Chemical Oxygen Demand Removal and Catholyte’s Copper Recovery
3.4. Morphologies of Electrode and XRD Analysis
3.5. Comparison of this Study with Previous Studies
Energy Substrates | Research Focuses | Reference | |
---|---|---|---|
Microbial Community of Anodic Biofilm | Wastewater (Containing Cu2+) | ||
acetate, lactate, ethanol, glucose | Yes, high throughput sequencing technique. | Yes | This study |
acetate, butyrate, propionate, glucose | Yes, using the culture-dependent technique. | No | [12] |
acetate, ethanol, glucose | No | No | [40] |
acetate, butyrate, glucose | Yes, using denaturing gradient gel electrophoresis. | No | [37] |
glucose, methanol, propyl alcohol | Yes, high throughput sequencing technique. | No | [13] |
acetate, glucose, starch, dextran, butyrate | No | No | [41] |
glucose, lactose, cheese | No | No | [42] |
glucose, acetate, xylose | No | No | [43] |
acetate | No | Yes | [44] |
acetate | No | Yes | [45] |
glucose | No | Yes | [46] |
acetate | No | Yes | [47] |
acetate | No | Yes | [48] |
acetate | No | Yes | [17] |
acetate | No | Yes | [49] |
acetate | No | Yes | [50] |
acetate | No | Yes | [51] |
acetate | Yes, high throughput sequencing technique; anodic biofilm was directly exposed to Cu2+. | Yes | [14] |
4. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Sample | Reads | Sobs | Shannon | Simpson | Ace | Chao | Coverage |
---|---|---|---|---|---|---|---|
Inoculum | 40818 | 710 | 5.2069 | 0.0138 | 732.31 | 735.02 | 0.9988 |
Glucose | 97701 | 205 | 0.7931 | 0.7633 | 363.79 | 347.38 | 0.9993 |
Acetate | 45985 | 590 | 2.9848 | 0.2534 | 670.25 | 672.18 | 0.9975 |
Ethanol | 43875 | 286 | 2.7412 | 0.1233 | 428.09 | 371.00 | 0.9981 |
Lactate | 32430 | 482 | 2.9694 | 0.2130 | 589.26 | 586.79 | 0.9963 |
Archaeal Genus | Percentage in Anode Biofilm (%) | ||||
---|---|---|---|---|---|
Inoculum | Glucose | Acetate | Ethanol | Lactate | |
Methanobacterium | 0 | 0.01 | 0 | 0.04 | 0.15 |
Methanobrevibacter | 0 | 1.82 | 0 | 23.70 | 5.23 |
Methanosaeta | 0 | 0 | 0.02 | 0 | 0.05 |
Methanosarcina | 0 | 0 | 0.19 | 0 | 0 |
Methanomassiliicoccus | 0 | 0 | 0.25 | 0 | 0.02 |
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Ai, C.; Yan, Z.; Hou, S.; Zheng, X.; Zeng, Z.; Amanze, C.; Dai, Z.; Chai, L.; Qiu, G.; Zeng, W. Effective Treatment of Acid Mine Drainage with Microbial Fuel Cells: An Emphasis on Typical Energy Substrates. Minerals 2020, 10, 443. https://doi.org/10.3390/min10050443
Ai C, Yan Z, Hou S, Zheng X, Zeng Z, Amanze C, Dai Z, Chai L, Qiu G, Zeng W. Effective Treatment of Acid Mine Drainage with Microbial Fuel Cells: An Emphasis on Typical Energy Substrates. Minerals. 2020; 10(5):443. https://doi.org/10.3390/min10050443
Chicago/Turabian StyleAi, Chenbing, Zhang Yan, Shanshan Hou, Xiaoya Zheng, Zichao Zeng, Charles Amanze, Zhimin Dai, Liyuan Chai, Guanzhou Qiu, and Weimin Zeng. 2020. "Effective Treatment of Acid Mine Drainage with Microbial Fuel Cells: An Emphasis on Typical Energy Substrates" Minerals 10, no. 5: 443. https://doi.org/10.3390/min10050443