Dual RNase and β-lactamase Activity of a Single Enzyme Encoded in Archaea
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sequence Analysis
2.2. Antibiotic Susceptibility Testing
2.3. In Vitro Assessment of the β-Lactamase Activity
2.4. Imipenem Antibiotic Degradation Monitored by Liquid Chromatography-Mass
2.5. DNAse and RNAse Activity Evaluation
2.6. Glyoxalase II Activity Assay
3. Results
3.1. Archaeal Class B Metallo-β-Lactamase
3.2. Characterization of the DNAse and RNAse Activities
3.3. Glyoxalase Activity
3.4. Archaeal Class C-Like β-Lactamases
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Diene, S.M.; Pinault, L.; Armstrong, N.; Azza, S.; Keshri, V.; Khelaifia, S.; Chabrière, E.; Caetano-Anolles, G.; Rolain, J.-M.; Pontarotti, P.; et al. Dual RNase and β-lactamase Activity of a Single Enzyme Encoded in Archaea. Life 2020, 10, 280. https://doi.org/10.3390/life10110280
Diene SM, Pinault L, Armstrong N, Azza S, Keshri V, Khelaifia S, Chabrière E, Caetano-Anolles G, Rolain J-M, Pontarotti P, et al. Dual RNase and β-lactamase Activity of a Single Enzyme Encoded in Archaea. Life. 2020; 10(11):280. https://doi.org/10.3390/life10110280
Chicago/Turabian StyleDiene, Seydina M., Lucile Pinault, Nicholas Armstrong, Said Azza, Vivek Keshri, Saber Khelaifia, Eric Chabrière, Gustavo Caetano-Anolles, Jean-Marc Rolain, Pierre Pontarotti, and et al. 2020. "Dual RNase and β-lactamase Activity of a Single Enzyme Encoded in Archaea" Life 10, no. 11: 280. https://doi.org/10.3390/life10110280