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Review

Is the Application of Plant Probiotic Bacterial Consortia Always Beneficial for Plants? Exploring Synergies between Rhizobial and Non-Rhizobial Bacteria and Their Effects on Agro-Economically Valuable Crops

by
Esther Menéndez
* and
Ana Paço
MED—Mediterranean Institute for Agriculture, Environment and Development, Institute for Advanced Studies and Research (IIFA), University of Évora, Pólo da Mitra, Ap. 94, 7006-554 Évora, Portugal
*
Author to whom correspondence should be addressed.
Life 2020, 10(3), 24; https://doi.org/10.3390/life10030024
Submission received: 31 January 2020 / Revised: 3 March 2020 / Accepted: 9 March 2020 / Published: 12 March 2020
(This article belongs to the Section Microbiology)
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Abstract

:
The overgrowth of human population and the demand for high-quality foods necessitate the search for sustainable alternatives to increase crop production. The use of biofertilizers, mostly based on plant probiotic bacteria (PPB), represents a reliable and eco-friendly solution. This heterogeneous group of bacteria possesses many features with positive effects on plants; however, how these bacteria with each other and with the environment when released into a field has still barely been studied. In this review, we focused on the diversity of root endophytic rhizobial and non-rhizobial bacteria existing within plant root tissues, and also on their potential applications as consortia exerting benefits for plants and the environment. We demonstrated the benefits of using bacterial inoculant consortia instead of single-strain inoculants. We then critically discussed several considerations that farmers, companies, governments, and the scientific community should take into account when a biofertilizer based on those PPBs is proposed, including (i) a proper taxonomic identification, (ii) the characterization of the beneficial features of PPB strains, and (iii) the ecological impacts on plants, environment, and plant/soil microbiomes. Overall, the success of a PPB consortium depends on many factors that must be considered and analyzed before its application as a biofertilizer in an agricultural system.

1. Why Do We Need More Sustainable Agriculture?

Currently, the global population is continuing to grow and is causing noticeable environmental damage, mostly driven by the misuse of natural resources due to the large ecological footprint of the humankind. According to the Food and Agriculture Organization (FAO) of the United Nations, the population will reach an unprecedented size in the coming years, mostly due to the high life expectancy in developed countries and to the high fertility of younger generations in developing and underdeveloped countries [1]. This situation makes increasing the food supply and a more equitable distribution of these food resources one of the prime concerns of our society [2,3].
Agriculture, which represents the primary sector globally and is crucial to feeding the population and livestock, covers more than one third of the world’s available land in total [1]. Even if more land could be dedicated to agriculture or used to create more intensive agricultural systems, there is no benefit to doing so, due to the severe negative effects that this land expansion and agricultural intensification might have [4]. In order to increase crop productivity, it is a common practice for farmers to increase the use of chemical fertilizers, namely nitrogen, phosphate, or potash fertilizers. In fact, the development of the technology for the production of chemical fertilizers in the 1930s to 1950s, as well as other technical improvements in agriculture driven by governments and companies, led to the so-called “Green Revolution” during the 1960s [4,5]. This “Green Revolution” created many advantages all over the world; however, in the long term, it brought negative consequences and disadvantages that are apparent today.
Based on the available data from FAOSTAT (Statistic Division of the Food and Agriculture Organization of the United Nations), in 2017, the five countries using the most nitrogen-based fertilizers (for agricultural use, in million (M) tonnes) were China (29.62 M), United States of America (USA; 11.58 M), Indonesia (2.95 M), Canada (2.47 M), and Vietnam (1.69 M). China and the USA were also the countries with the highest use of P-(P2O5) (15.37 M and 4.27 M. respectively) and K (K2O)-based fertilizers (13.59 M and 4.79 M, respectively). Canada, Australia, Malaysia, Indonesia, and Vietnam also appeared within the top 10 countries using these kinds of chemical fertilization [1]. According to the FAOSTAT database, there was an increase in the use of N-, P-, and K-based fertilizers between 2006 and 2016 in every area in the world (Figure 1A). The average use of chemical fertilizers per unit area of cropland is reaching an especially alarming level in Asia and America (both North and South America).
Sugarcane, grain legumes, maize, wheat, rice, and vegetables are included in the top 10 commodities worldwide, representing the major part of crop production and occupying most of the arable land available, with production increasing significantly in some cases in recent years (Figure 1B). Moreover, other crops such as quinoa, ginger, and rapeseed (oilseeds) are reaching significant levels in terms of crop establishment [6,7], leading to higher productions and more arable land being covered with these crops, replacing other less “profitable” ones. Interestingly, the area harvested and the land area dedicated to major crops did not increase during the 2006–2016 period. These facts may suggest that the increase in crop production was related mostly to the application of fertilizers, although there are other factors to consider that might have contributed, such as the use of crop varieties with improved features (use of stress-tolerant varieties and/or naturally adapted or genetically modified (GMO) crops, amongst others) [8,9,10].
The application of chemical fertilizers is causing harmful effects to the environment, such as soil acidification, water eutrophication, and air pollution, amongst other problems [3,11]. Thus, it is becoming crucial to achieve higher production through sustainable practices, avoiding or at least reducing the use of chemical fertilizers. The use of “greener” fertilizers, namely biofertilizers, mostly based on plant probiotic bacteria (PPB), represents a solution to most of the problems that result from the application of chemical fertilizers [12]. This heterogeneous group of beneficial bacteria has many features with positive effects on plants (Figure 2). Within the PPBs, there are bacteria able to perform biological nitrogen fixation (BNF), known as nitrogen-fixing bacteria, others capable of solubilizing P and K forms that are not available to plants, known as P- or K-solubilizing bacteria (PSB and KSB, respectively). Moreover, the ability to biosynthesize phytohormones and siderophores are two of the most common plant-growth-promoting mechanisms displayed by PPBs [13,14,15]. Furthermore, PPBs also have biocontrol properties, acting as plant-protecting agents against pathogens of different nature. The production of siderophores and cell-wall-hydrolytic enzymes (also considered plant growth promotion mechanisms), as well as the emission of volatile compounds (VOCs) and the production of antimicrobial compounds, are among those biocontrol properties [16]. The use of PPBs not only improves crop production, but also the quality of the grains, fruits, or products derived from these crops [17].
The potential use of these beneficial bacteria in biofertilization schemes has caught the eye of the society in general. Farmers need to obtain high yields of healthier crops in response to the increased food demand of consumers [4]. Moreover, governments have the responsibility to create policies governing the use of sustainable and eco-friendly fertilizers [4]. Biotech companies such as Bayer, Novozymes, and BASF, amongst others, develop and commercialize biofertilizers based on PPBs, this being a blooming and profitable market [4]. The production and application of single and mixed inoculants based on selected plant probiotic bacteria is now very attractive at both economic and ecological levels, representing a real alternative for the sustainable reduction of the use of synthetic fertilizers in fields [12,18].

2. The Plant Microbiome, with a Focus on the Roots and Endophytic Plant Probiotic Bacteria

Plants harbor a complex microbiome within their inner tissues, which can be considered a second genome. A plant without any associated microbiota is very rare in nature [19]. Plants and their microbiomes can behave as one entity, which has also close relationships with the environment, namely with the so-called rhizosphere microbiome [20,21]. The term coined to refer to this “intimate partnership” is the plant holobiont [22]. The scientific community is invested in the understanding on how the plant holobiont works, where microbiomes come from, and how to manage them in order to obtain some profit for agricultural systems [23,24,25,26,27,28,29,30,31,32].
Here, we focused on a subset of the plant microbiome: the bacteria displaying endophytic behavior from rhizobial (rhizobial bacterial endophytes; RBE) and non-rhizobial (non-rhizobial bacterial endophytes; NRBE) origins; these are also considered plant probiotic bacteria, since they promote plant growth using different mechanisms. This group of endophytic bacteria is very diverse and heterogeneous, and members commonly belong to the phyla Proteobacteria, Firmicutes, Actinobacteria, and Bacteroidetes [23]. These bacteria easily enter plant roots through different entry mechanisms (Figure 2), such as intracellular invasion, crack entry, or root hair invasion, the latter being commonly associated with rhizobial species entering legume roots [33,34]. Although the presence of plant probiotic endophytic bacteria in shoots, leaves, flowers, and seeds of plants is also regularly studied [35], in this review we focused on the root endophytic bacteria; some such endophytes might even travel actively or passively to the upper parts of the plants via vascular tissues [36,37].
In this work, have recurrently mentioned the microbiome from legume root nodules, because besides accommodating rhizobia strains, these nodules can also be occupied by diverse non-rhizobial bacteria [38,39,40]. Interestingly, there have been some works proposing the microbiomes of legume nodules to be key sources of endophytic PPBs (RBEs and NRBEs), claiming that this particular legume organ harbors a wide range of beneficial endophytes with plant growth promotion features that fulfil biosafety rules [40,41,42].

3. Plant Probiotic Bacteria with Potential for Improving Crop Yields: Which Are They and How Do They Promote Plant Growth?

3.1. What Are the RBEs?

Rhizobia are a complex group of Gram-negative bacteria that live in the soil as saprophytes or in association with plants, as endophytes of inner plant tissues. These bacteria have the amazing availability to fix atmospheric nitrogen when located within the nodule and to convert this nitrogen into available nitrogen compounds to be used directly by the legumes, these features having been extensively studied for decades [43,44]. As well as this ability, rhizobia also possess further features that are beneficial for plant development, whether in free-living conditions (rhizosphere) or forming biofilms in the rhizoplane or in association with plants as endophytes [45,46]. Moreover, rhizobia are able to protect the plant host against pathogens, functioning as biocontrol agents [47,48,49].
Recent studies have identified the orders Rhizobiales (α-rhizobia), and Burkholderiales (ß-rhizobia) as keystone taxa, since they are always present in the core microbiomes of plants. The genera Rhizobium and Bradyrhizobium, belonging to the order Rhizobiales, and the genus Burkholderia, belonging to the order Burkholderiales, are the ones with better-known functions in improving the plant productivity and maintaining community evenness [50,51].
There is a plethora of previous work showing the beneficial effects of rhizobial strains on the growth and development of several non-legume crops, such as sorghum, lettuce, carrots, spinach, tomato, pepper, strawberries, barley, wheat, rice, and cotton, amongst others, improving in most of these crops the quality of both the edible and non-edible parts [52,53,54,55,56,57,58,59,60]. The majority of these studies have shown the effects of these interactions only under laboratory- or greenhouse-controlled conditions.

3.2. What Are the NRBEs?

The beneficial non-rhizobial bacterial endophytes are taxonomically very diverse, and their functions are commonly associated with their plant-growth-promoting activities. They are also known as “helpers”, improving the effectiveness of the rhizobium–legume symbiosis [61,62].
Pseudomonadales, Enterobacteriales, and some Bacilli and Paenibacilli are among the NRBE taxa most commonly found in below-ground plant structures, whether the approaches analyzing microbiomes are culture-dependent or culture-independent. As an example of a culture-dependent study, Brígido et al. [63] showed that the genera Enterobacter and Pseudomonas were the most frequently identified genera in chickpea roots. Moreover, Trujillo et al. [64] identified the genus Micromonospora as being commonly associated with legume nodules, and Muresu et al. [65] showed that the NRBE-populated nodules of several legumes included at least the genera Bacillus, Pseudomonas, Xanthomonas, Agromyces, Microbacterium, Curtobacterium, and Micromonospora, and members of the family Enterobacteriaceae. Interestingly, de Meyer et al. [66] reported the concurrence of some rhizobia with some of the identified NRBEs, which belonged to the genera Pseudomonas, Bacillus, and Paenibacillus, among the most frequently isolated NRBEs from the nodules of 30 different leguminous plants.
In culture-independent studies, apart from the already stated Rhizobiales, commonly found in root microbiomes [46], the orders Sphingomonadales, Enterobacteriales, Burkholderiales (NRBE), Caulobacterales, and Rhodospirillales, from the phylum Proteobacteria, and the genus Syntrophomonas from the phylum Firmicutes are the most abundant in Trifolium entire roots [67]. Proteobacteria and Firmicutes are the phyla with the highest operational taxonomic units (OTU) abundancies in the rice root endosphere microbiome [68]. In maize roots, the phylum Proteobacteria accounts for approximately the 85% of the total root microbiome [69]. In this study, the family Enterobacteriaceae and the genera Burkholderia (we denoted the latter genus as NRBE, but its symbiotic members were considered ß-rhizobia [70]), Herbaspirillum, Curvibacter, Acinetobacter, Stenotrophomonas, and Pseudomonas (Proteobacteria) and Curtobacterium (Actinobacteria) were significantly enriched within maize roots.
Overall, the genus Pseudomonas and its closely related genera seem to be the most abundant genera in root microbiomes analyzed by both culture-dependent and -independent approaches. The members of this genus have been extensively studied and display a complete range of beneficial traits for plants. For instance, Hu et al. [71] suggested that probiotic Pseudomonas communities may manage the functionality of the microbial communities associated with tomato via regulation of important plant-growth-promoting traits. Moreover, some authors have attributed the capacity to form nodules in legumes to some strains of this genus, as well as to some Paenibacillus and Enterobacteria strains [40,72,73,74]. However, these studies must be taken very cautiously, as they showed no conclusive proof or correct controls. The affirmation of the nodulation capacity attributed to these strains must be supported with tests that provide unequivocal proof of this particular ability [75].

3.3. How Do They Promote Plant Growth?

There are many reviews offering an overview of the vast information about microorganisms, and specially bacteria, that are plant growth promoters and biocontrol agents, and also reviewing the mechanisms associated with these particular functions, as well as more publications showing and/or reviewing the benefits to plants, such as the enhancement of bioactive compounds in the edible parts, among others [3,12,13,14,15,76,77,78,79,80,81,82,83].
Briefly, PPBs have features involved in the (i) facilitation of nutrient acquisition, (ii) production of phytohormones and modulation of their levels, (iii) tolerance to either abiotic or biotic stresses, (iv) production of siderophores and other metabolites, and (v) induction of disease resistance, among other properties not listed here (Figure 2). The expression of these promotion activities when RBEs and NRBEs are in association outside or inside the plant tissues will make the difference in most cases with a single inoculation of either RBEs or NRBEs separately.

4. Better Together—Inoculation with PPB Consortia vs. Individual PPBs

Nowadays, it is accepted that bacteria function mostly as consortia in diverse natural microbiomes. However, the molecular basis of plant–NRBE/RBE associations remains poorly understood, at least compared to what is known about the symbiotic relationships between legumes and rhizobia or plants and their pathogenic bacteria [78,84]. These relationships that occur between plants and microbes have high levels of complexity. The addition of a given bacterial consortium will increase the complexity of those systems, more so than if only a single strain is inoculated [85].
Many studies have reported co-inoculation assays of NRBE and RBE strains in plants, and have proven that inoculating PPBs in consortia provides many benefits to plants [86,87,88,89,90]. However, most of these studies were performed in lab- and greenhouse-controlled conditions, only a small proportion having been performed under field conditions (Table 1 and references cited therein). In fact, the improvement of symbiotic effectiveness of RBE by co-inoculation with other beneficial NRBEs [91,92,93,94,95], as well as other RBEs forming a consortium [96,97] are commonly observed showing that the strains might assume one or functions depending on many factors. Moreover, there are studies combining RBE/NRBEs with mycorrhiza, showing improved results in yields and quality, as well as reducing the amount of chemical fertilization [98,99,100,101,102,103].
In the particular case of legumes, RBEs and NRBEs living within nodules certainly act synergistically to increase plant growth, health, and survival [40,41]. Interestingly, some studies show that the taxonomic composition of the nodule microbiome varies depending on which rhizobium has induced the nodules. For instance, Lu et al. [99] showed significant differences in rhizosphere and nodule bacterial communities when Dalbergia plants were nodulated with Rhizobium, Bradyrhizobium, or Burkholderia strains. Dominant OTUs found were Lactococcus, Pseudomonas, Bacillus, and Cyanobacteria. Moreover, these authors found differences in relative abundances when nodules were formed in N-fed or N-limited conditions. The benefits of RBEs and NRBEs associations are not restricted to legumes, extending also to non-legume species. For example, the co-inoculation of Bacillus and Pseudomonas (two NRBEs) in tomato plants increased yield and enhanced the nutrient content of tomato fruits [117]. Interestingly, the inoculation of a given NRBE strain (Bacillus) did not alter bacterial communities associated with tomato roots in the long term [118], which would be good in agricultural systems for the maintenance of native microbiomes, because the microbial community will absorb the added strain after this strain produces the beneficial effect to the plant.
Taking all the above into account, we may assume that the benefits of mixed inoculation happen due to the combination of plant-growth-promoting activities and, in the case of the combination of RBEs and NRBEs, the non-rhizobial bacteria act to favor functions clearly attributed to rhizobia, such as the nodulation and nitrogen fixation. Therefore, plant co-inoculations with RBE and NRBE consortia have benefits in the development of both legume and non-legume plants, which highlights that these consortia are efficient biofertilizers for use in the future, in a more sustainable mode of agriculture. Nevertheless, there is no magical combination, since the beneficial effects shown in nodulation and yield when a certain non-rhizobial strain is co-inoculated with a rhizobial strain may not display the same beneficial effect in another crop, or may not produce significant increases as it is expected. For instance, Camacho et al. [119] showed that a Bacillus strain, considered a proper PGPR, was beneficial in the R. tropici–Phaseolus vulgaris symbiosis, but had deleterious effects when interacting with the B. japonicum–Glycine max relationship. Table 1 also shows some other examples in which the effects on plant growth and yield derived from inoculation of PPB consortia in field tests were not always beneficial or did not produce significant increases of yield or quality [87,88,110,111,112,113,114,115]. These negative findings might be due to the enormous variety of factors affecting crops, especially in the field, where microbiomes and plants are subjected to biotic and abiotic stresses. The use of different “omics” techniques, including metagenomics or transcriptomics, will elucidate which are the mechanisms underlying both beneficial and deleterious effects. Thus, there is a need for future work on several aspects and considerations, which are discussed in the next section.

5. Considerations for More Efficient Use of PPB Consortia in the Fields

An overwhelming number of studies has been published about the isolation of new plant-growth-promoting bacteria, some of which showed outstanding benefits for plant growth. Moreover, as the omics techniques, such as metagenomics or genome sequencing, are becoming more economically accessible for to scientific community (companies are offering cheaper prices per sample), the number of works deciphering plant and soil microbiomes is increasing [120]. Undoubtedly, knowledge is of paramount importance, but it is necessary to acquire a deeper understanding about the molecular dialogues among plants, microbes, and how these interactions are shaped by the environment. Moreover, not all bacteria detected by metagenomics and other omics might be suitable for commercial inoculants because they could be impossible or difficult to grow in standard laboratory conditions [121,122].
There are research areas that must be prioritized, such as the study of crop microbiomes globally, the development of host–microbiome models to study functions and possible interactions, and abandoning the idea of a single solution that will fit all cases [27,40]. For instance, Kong et al. [123] proposed the use of simplified microbial consortia (SMCs) in order to improve crop yields. The microbial components of such SMCs should be selected from the core microbiomes and the rhizosphere microbiomes of crops through omics approaches. Synergies among SMC components, plant growth promoting activities, and changes in natural population dynamics induced by them should then be tested. Apart from this “microbial modeling” for the design of a successful biofertilizer composed of a given consortium, other authors have also considered the formulation and the appropriateness of the carrier/delivery system to the crops as key points to take consider [124]. Here, we focused on some of the points that we think are currently less discussed, and that we consider to be priorities: a reliable taxonomic and functional identification of the strains forming part of a consortium with possibilities of application, and the ecological constraints that will appear upon inoculation of bacteria consortia to improve crop yields.

5.1. Reliable Identification and Characterization of Inoculant Strains

Bashan et al. [125] proposed a series of rules that authors of new manuscripts about plant protection products should follow, including taxonomy rules, formulation content, and details of the application methods. Some authors are reluctant to correctly identify bacterial strains, arguing that their studies are not about bacterial taxonomy and/or systematics. The correct taxonomic identification of the given strains that are present or not in a consortium must be resolved from the beginning of a particular study. A correct identification must also be performed when fungal or algal species are included in the formulations. Misidentification of strains and problems regarding taxonomical affiliations are more common than should be allowed. Why do we need to identify our isolates correctly? It is all about biosafety and environmental awareness. For instance, some of the examples given in Table 1, such as the inoculation of crops with strains of Pseudomonas aeruginosa [108], among others, might represent a biosafety conflict, as many strains belonging to these species are pathogenic to humans and animals.
In recent decades, the biofertilizer market has grown exponentially, and governments have enacted many policies and laws concerning the marketing and application of these products [4,126]. Companies and scientists developing such products are required to follow these biosafety rules. Some countries are more permissive, some countries are very strict, and some of them do not have policies regulating these products yet. Big chemical fertilizer companies as well as new SMEs are now interested in the production and marketing of microbe-based products [127]. For example, the European Commission (EC) has approved regulations and policies governing the use of biofertilizers based on microorganisms; however, these policies are restrictive in terms of the permitted microorganisms, maybe due to the presence of strong fertilizer industries [127]. In June 2019, the EC enacted the “Regulation (EU) 2019/1009 of the European Parliament and of the Council of 5 June 2019 laying down rules on the making available on the market of EU fertilizing products”, which amended Regulations (EC) No 1069/2009 and (EC) No 1107/2009 and repealed Regulation (EC) No 2003/2003. This Regulation has a section in which only four kinds of microorganism are acceptable as part of a biofertilizer: Azotobacter spp., Azospirillum spp., Rhizobium spp. and mycorrhiza (Annex II). Nevertheless, Article 42 “Amendment of Annexes” provides the basis for adding more microorganisms to the list of those allowed. This article contains a checklist that must be completed before the registration of a PPB-based product, including the correct and complete taxonomical classification of the microorganisms, as well as proofs confirming these microorganisms to be “Generally Recognized as Safe” (GRAS).

5.2. Ecological Constraints that Might Affect Inoculant Functioning in the Field

Most studies describing RBE and NRBE consortia are still performed under controlled conditions in labs and greenhouses, without any further study about the ecological implications or survival under field conditions [128]. The effects produced among components of a consortium and/or between single or mixed bacteria with a plant depend mostly on biotic and abiotic factors [19]. The effect of a bacterium or a consortium might be specific or restricted to some conditions as well as their establishment as endophytes; some strains or mixes of strains do not always achieve the desired endophytic lifestyle. For instance, different strains of the genus Bacillus have different effects when inoculated on broccoli sprouts under field conditions. These strains did not establish themselves as endophytes, but some of them promoted plant growth promotion and influenced the bacterial communities [129]. Moreover, interactions with other microorganisms in the soils and plant tissues, such as fungi, oomycetes, and other bacteria, must be considered. For example, the PPB strains of the genus Azospirillum showed good plant-growth-promoting attributes but a less good capacity to compete with other bacteria associated with the plants [130].
The ecological impact generated when a field is inoculated with a PPB-based consortium must be addressed before the inoculation. For example, the addition of either N fertilizers or Rhizobium-based fertilizers significantly reduced the OTU richness and relative abundances in rice roots [131]. In this work, the rice–endophytic community presented genera Rheinheimera, unclassified Rhodospirillaceae, Pseudomonas, Asticcacaulis, Sphingomonas, and Rhizobium as dominant taxa. This community may have been influenced by the positive synergistic impact of rhizobial biofertilizer inoculation (with one strain belonging to the genus Rhizobium) in combination with low N fertilization. Indeed, the combined treatment showed the greatest vegetative growth promotion. Although this study was performed in a greenhouse, the use of soils directly extracted from the cultivation fields meant that the obtained results were closer to reality.
Interestingly, it is generally assumed that the inoculation of free-living diazotrophic bacteria has less impact on soil and plant microbiomes than the inoculation of rhizobial endosymbionts, because of the symbiotic specialization of the latter group [130]. The success of a given PPB consortium/biofertilizer relies on its specific interactions with the plants, which are mediated by plant exudates and the ability of the PPBs to compete with other microbes [130]. If the PPBs are true beneficial endophytes and are able to colonize plant roots, they will be capable of altering the natural microbial community. This fact could lead to a two-sided effect, which might be good or bad for the communities, depending on what, how, and when the effects are evaluated.

6. Conclusions

The use of plant probiotic RBEs and NRBEs as inoculants has the potential to increase crop yield without the overapplication of chemical fertilizers, pesticides, and fungicides, and consequently to reduce the environmental impact in agriculture and maximize the production of heathier and safer foods. RBEs and NRBEs establish synergies and act complementarily when forming a consortium, providing beneficial effects to crops. Although a high number of these associations are well-documented and most of them show positive results, there is still a lack of knowledge on how these consortia behave and interact with plants, environments, and other microbes (the microbiome). Nonetheless, there are many consortia already being commercially exploited. We may conclude that the use of these beneficial bacteria combined in consortia is very important for improving crop yields and performance. However, the overall ecological impact is still not well-known and must be addressed in future studies if the successful and sustainable application of bacterial consortia is to be pursued for a better, greener, and more profitable agriculture.

Author Contributions

Conceptualization, E.M.; Reviewing of literature, A.P. and E.M.; Writing—original draft preparation, A.P. and E.M.; Writing—review & editing, A.P. and E.M. All authors have read and agreed to the published version of the manuscript.

Funding

This work was also funded by FEDER—Fundo Europeu de Desenvolvimento Regional funds through the COMPETE 2020—Operational Programme for Competitiveness and Internationalisation (POCI), and by Portuguese funds through FCT - Fundação para a Ciência e a Tecnologia in the framework of the project POCI-01-0145-FEDER-016810 (PTDC/AGR-PRO/2978/2014 and the Project UIDB/05183/2020. EM acknowledges a FCT contract from the Individual Call to Scientific Employment Stimulus 2017 (CEECIND/00270/2017).

Acknowledgments

The authors acknowledge Paula Garcia-Fraile (University of Salamanca) for critically revise the draft manuscript.

Conflicts of Interest

The authors declare no conflict of interest. The funders have no role in the design of the study; in the collection, analyses or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.

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Figure 1. Graphical representation of data available on FAOSTAT. (A) Average use of fertilizers per area of cropland (kg/ha): from left to right, data regarding to N-based, phosphate, and potash, all of which are synthetic fertilizers. Graph shows the difference between 2006 and 2016 in broad areas of the World, as well as the average use in 2006 and 2016 worldwide. (B) Data regarding to the most produced commodities all over the world and the area harvested worldwide.
Figure 1. Graphical representation of data available on FAOSTAT. (A) Average use of fertilizers per area of cropland (kg/ha): from left to right, data regarding to N-based, phosphate, and potash, all of which are synthetic fertilizers. Graph shows the difference between 2006 and 2016 in broad areas of the World, as well as the average use in 2006 and 2016 worldwide. (B) Data regarding to the most produced commodities all over the world and the area harvested worldwide.
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Figure 2. Plant-growth-promoting attributes and entry mechanisms showed by plant probiotic bacteria (PPBs) on plants. Both RBEs (rhizobial bacterial endophytes) and NRBEs (non-rhizobial bacterial endophytes) can exert one or several plant-growth-promoting traits. The success in the colonization and infection of these bacteria will result in a better plant performance. The root hair entry mechanism is usually associated with RBEs. NRBEs might also enter actively by root hairs, by just erode the cell wall, forming (or not) a tubular structure similar to an infection thread (IT), or passively, via the IT formed by rhizobia. Both RBEs and NRBEs might also enter actively or passively by the other mechanisms described in the figure.
Figure 2. Plant-growth-promoting attributes and entry mechanisms showed by plant probiotic bacteria (PPBs) on plants. Both RBEs (rhizobial bacterial endophytes) and NRBEs (non-rhizobial bacterial endophytes) can exert one or several plant-growth-promoting traits. The success in the colonization and infection of these bacteria will result in a better plant performance. The root hair entry mechanism is usually associated with RBEs. NRBEs might also enter actively by root hairs, by just erode the cell wall, forming (or not) a tubular structure similar to an infection thread (IT), or passively, via the IT formed by rhizobia. Both RBEs and NRBEs might also enter actively or passively by the other mechanisms described in the figure.
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Table
Table 1. Examples of plant probiotic bacteria applied as consortia and tested on field conditions.
Table 1. Examples of plant probiotic bacteria applied as consortia and tested on field conditions.
Consortium CompositionPlant Host and ConditionsEffects on
Tested Crops		Reference
RBE + NRBE
Bradyrhizobium japonicum + Rhizobium tropici +Azospirillum brasilenseSoybean and common bean field trials in Brazil. Azospirillum applied in-furrow, rhizobia applied on seeds.Increases in grain yield: 16.1% soybean/19.6% common bean respective to indigenous rhizobial population, and 7.1% soybean/14.7% common bean respective to rhizobia-inoculated treatment.[104]
Rhizobium and 2 Bacillus (B. subtilis + B. megaterium)Common bean fields trials in Eastern AnatoliaNo significant effect on common bean yield compared with single inoculations.[105]
R. leguminosarum + Bacillus sp. + Pseudomonas sp.Phaseolus vulgaris/two field trialsAverage grain yield was enhanced by 39% after application of consortium.[106]
R. leguminosarum +
P. fluorescens		Lentil field trials (2) with different P fertilization levelsImproved symbiotic parameters, leghemoglobin content, growth, and grain yield of lentil with 50% P reduction.[107]
Bradyrhizobium + P. aeruginosaSoybean field assayImproved plant growth but not to a statistically significant degree. Nodule weight was significantly higher in the co-inoculated soybean treatment.[108]
Mesorhizobium + P. fluorescens or P. aeruginosa/
Azotobacter chroococcum/
B. megaterium		Chickpea field assays Improvements depended on the partner PGPR. Combination with Pseudomonas showed best results.[88]
Rhizobium + Sinorhizobium +
Bacillus + Burkholderia		Pidgeon pea (Cajanus cajan) field trialIncreased plant biomass and nodule mass per plant.[93]
NRBE
Arthrobacter nitroguajacolicus + B. cereus + B. megaterium + B. mojavensis + P. azotoformans + P. frederiksbergensisTwo field plots of Nicotiana attenuata with fungal disease problemsSignificantly reduced disease incidence and mortality in the infected field plot without influencing growth or herbivore resistance[109]
Mixes of various Pseudomonas, Enterobacter and Serratia strainsRapeseed field trials (also greenhouse trials)Not statistically significant increases of rapeseed oil and grain yields[110]
Pseudomonas sp. + Chryseobacterium sp.Rice, paddy. Three cropping seasons of a rice fieldSignificantly increased rice production and enhanced, but not to a significant degree, the quality of the rice grains. Protected against rice blast fungus.[111]
Various consortia involving Enterobacter, Serratia, Pseudomonas, Microbacterium and AchromobacterAvocado seedlings grown in a nursery located outdoors and within a commercial avocado orchardMitigate water shortage and salt stress. No report on plant biomass or yield increments.[112]
Rhodotorula graminis + Rahnella sp. + Burkholderia sp. + Acinetobacter calcoaceticus + R. tropici + Sphingomonas yanoikuyae + P. putida + Sphingomonas sp.Production of Douglas fir plantlets in a nurseryNot significant increases in weight of shoot and N content in needles.[113]
RBE
2 Burkholderia sp.Fenugreek field trialThe grain yield was enhanced by 40% as compared to control in field trials. Inhibited Fusarium spp.[114]
Occhrobactrum ciceri + Mesorhizobium ciceroDifferent kabuli and desi type chickpea genotypes in a field trial in two different soilsOne Desi genotype showed increased nodulation in plants co-inoculated. Increased biomass and grain yield in every genotype.[115]
Various consortia of 2
R. leguminosarum bv trifolii strains		Extensive rice field trials in the Egyptian Nile DeltaSignificant increases in straw biomass. Reduction of the use of synthetic N fertilizers.[116]
RBE/NRBE with Mycorrhiza
2 Pseudomonas + mixed mycorrhizaOpen field trial in a real industrial tomato farmIncreases of flowering, dimensions, and weight of tomato fruits and improved industrial and nutritional features of fruits.[100]
Commercialized consortia of Azotobacter vinelandii and Mycorrhyza (Rhizophagus irregularis)Common wheat field trials with different NPK fertilization ratesWhen inoculated with medium and high fertilization, promoted cluster shift according to vectors/variables related to root growth and nutrient allocation in the grains and limits root growth at low fertilization levels.[101]
Acinetobacter sp., Rahnella aquatilis, 2 strains of Ensifer meliloti and Glomus + Sclerocystis + AcaulosporaWheat and Vicia faba field trialsApplication of either only PGPB alone or PGPB + mycorrhyza increased parameters.[102]
P. fluorescens + Azospirillum lipoferum + Glomus intrarradicesMaize field trials. Fertilized, reduced, and not fertilized. No increase in production or plant biomass. Restored production with the combination 50% fertilizer + consortium.[103]

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Menéndez, E.; Paço, A. Is the Application of Plant Probiotic Bacterial Consortia Always Beneficial for Plants? Exploring Synergies between Rhizobial and Non-Rhizobial Bacteria and Their Effects on Agro-Economically Valuable Crops. Life 2020, 10, 24. https://doi.org/10.3390/life10030024

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Menéndez E, Paço A. Is the Application of Plant Probiotic Bacterial Consortia Always Beneficial for Plants? Exploring Synergies between Rhizobial and Non-Rhizobial Bacteria and Their Effects on Agro-Economically Valuable Crops. Life. 2020; 10(3):24. https://doi.org/10.3390/life10030024

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Menéndez, Esther, and Ana Paço. 2020. "Is the Application of Plant Probiotic Bacterial Consortia Always Beneficial for Plants? Exploring Synergies between Rhizobial and Non-Rhizobial Bacteria and Their Effects on Agro-Economically Valuable Crops" Life 10, no. 3: 24. https://doi.org/10.3390/life10030024

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