Mapping of Photochemically-Derived Dityrosine across Fe-Bound N-Acetylated α-Synuclein
Abstract
:1. Introduction
2. Materials and Methods
2.1. Transformation of Expression Strains for NAcαSyn Variants
2.2. Expression and Protein Purification
2.3. Photochemical Crosslinking of Unmodified Proteins (PICUP)
3. Results and Discussion
3.1. Photoinduced Dityrosine Formation of NAcαSyn Variants in the Absence of Iron
3.2. Photoinduced Dityrosine Formation of Fe-Bound NAcαSyn Variants
3.3. Gel Analysis of PICUP Reaction Products
4. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Appendix A
References
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Curry, A.M.; Fernàndez, R.D.; Pagani, T.D.; Abeyawardhane, D.L.; Trahan, M.L.; Lucas, H.R. Mapping of Photochemically-Derived Dityrosine across Fe-Bound N-Acetylated α-Synuclein. Life 2020, 10, 124. https://doi.org/10.3390/life10080124
Curry AM, Fernàndez RD, Pagani TD, Abeyawardhane DL, Trahan ML, Lucas HR. Mapping of Photochemically-Derived Dityrosine across Fe-Bound N-Acetylated α-Synuclein. Life. 2020; 10(8):124. https://doi.org/10.3390/life10080124
Chicago/Turabian StyleCurry, Alyson M., Ricardo D. Fernàndez, Talita D. Pagani, Dinendra L. Abeyawardhane, Morgan L. Trahan, and Heather R. Lucas. 2020. "Mapping of Photochemically-Derived Dityrosine across Fe-Bound N-Acetylated α-Synuclein" Life 10, no. 8: 124. https://doi.org/10.3390/life10080124