Biologic Impact of Different Ultra-Low-Fluence Irradiations in Human Fibroblasts
Abstract
:1. Introduction
2. Materials and Methods
2.1. Human Cells and Culture Conditions
2.2. Pretreatment with Ultra-Low-Fluence Irradiation
2.3. Proton Microbeam Irradiation
2.4. Cell Death Assessment
2.5. Analysis of Mutation Frequency
2.6. Statistical Analysis
3. Results
4. Discussion
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
- Pierce, D.A.; Preston, D.L. Radiation-related cancer risks at low doses among atomic bomb survivors. Radiat. Res. 2000, 154, 178–186. [Google Scholar] [CrossRef] [Green Version]
- Evans, H.H.; Horng, M.F.; Ricanati, M.; Diaz-Insua, M.; Jordan, R.; Schwartz, J.L. Induction of genomic instability in TK6 human lymphoblasts exposed to 137Cs γ radiation: Comparison to the induction by exposure to accelerated 56Fe particles. Radiat. Res. 2003, 159, 737–747. [Google Scholar] [CrossRef]
- Snyder, A.R.; Morgan, W.F. Radiation-induced chromosomal instability and gene expression profiling: Searching for clues to initiation and perpetuation. Mutat. Res. 2004, 568, 89–96. [Google Scholar] [CrossRef] [PubMed]
- Urushibara, A.; Kodama, S.; Suzuki, K.; Desa, M.B.M.; Suzuki, F.; Tsutsui, T.; Watanabe, M. Involvement of telomere dysfunction in the induction of genomic instability by radiation in scid mouse cells. Biochem. Biophys. Res. Commun. 2004, 313, 1037–1043. [Google Scholar] [CrossRef]
- Canova, S.; Fiorasi, F.; Mognato, M.; Grifalconi, M.; Reddi, E.; Russo, A.; Celotti, L. “Modeled microgravity” affects cell response to ionizing radiation and increases genomic damage. Radiat. Res. 2005, 163, 191–199. [Google Scholar] [CrossRef]
- Gowans, D.; Lorimore, S.A.; McIlrath, J.M.; Wright, E.G. Genotype-dependent induction of transmissible chromosomal instability by γ-radiation and the benzene metabolite hydroquinone. Cancer Res. 2005, 65, 3527–3530. [Google Scholar] [CrossRef] [Green Version]
- Jagetia, G.C.; Venkatesha, V.A. Effect of mangiferin on radiation-induced micronucleus blood lymphocytes. Environ. Mol. Mutagen. 2005, 46, 12–21. [Google Scholar] [CrossRef]
- Tsai, M.H.; Chen, X.; Chandramouli, G.V.R.; Chen, Y.; Yan, H.; Zhao, S.; Keng, P.; Liber, H.L.; Coleman, C.N.; Mitchell, J.B.; et al. Transcriptional responses to ionizing radiation reveal that p53R2 protects against radiation-induced mutagenesis in human lymphoblastoid cells. Oncogene 2006, 25, 622–632. [Google Scholar] [CrossRef] [Green Version]
- Suzuki, M.; Tsuruoka, C.; Uchihori, Y.; Ebisawa, S.; Yasuda, H.; Fujitaka, K. Reduction in life span of normal human fibroblasts exposed to very low-dose-rate charged particles. Radiat. Res. 2005, 164, 505–508. [Google Scholar] [CrossRef]
- Oliveri, G.; Bodycote, J.; Wolff, S. Adaptive response of human lymphocytes to low concentrations of radioactive thymidine. Science 1984, 223, 594–597. [Google Scholar] [CrossRef]
- Stoilov, L.M.; Mullenders, L.H.; Darroudi, F.; Natarajan, A.T. Adaptive response to DNA and chromosomal damage induced by X-rays in human blood lymphocytes. Mutagenesis 2007, 22, 117–122. [Google Scholar] [CrossRef] [PubMed]
- Ito, M.; Shibamoto, Y.; Ayakawa, S.; Tomita, N.; Sugie, C.; Ogino, H. Low-dose whole-body irradiation induced radioadaptive response in C57BL/6 mice. J. Radiat. Res. 2007, 48, 455–460. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Day, T.K.; Zeng, G.; Hooker, A.M.; Bhat, M.; Scott, B.R.; Turner, D.R.; Sykes, P.J. Adaptive response for chromosomal inversions in pKZ1 mouse prostate induced by low doses of X radiation delivered after a high dose. Radiat. Res. 2007, 167, 682–692. [Google Scholar] [CrossRef] [PubMed]
- Krishnaja, A.P.; Sharma, N.K. Variability in cytogenetic adaptive response of cultured human lymphocytes to mitomycin C, bleomycin, quinacrine dihydrochloride, Co60 gamma-rays and hyperthermia. Mutagenesis 2008, 23, 77–86. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Jiang, H.; Li, W.; Li, X.; Cai, L.; Wang, G. Low-dose radiation induces adaptive response in normal cells, but not in tumor cells: In vitro and in vivo studies. J. Radiat. Res. 2008, 49, 219–230. [Google Scholar] [CrossRef] [Green Version]
- Elmore, E.; Lao, X.Y.; Kapadia, R.; Giedzinski, E.; Limoli, C.; Redpath, J.L. Low doses of very low-dose-rate low-LET radiation suppress radiation-induced neoplastic transformation in vitro and induce an adaptive response. Radiat. Res. 2008, 169, 311–318. [Google Scholar] [CrossRef]
- Kilemade, M.; Lemon, J.; Boreham, D. Characteristics of the adaptive response in cultured salmon cells exposed to ionizing radiation. Environ. Mol. Mutagen. 2008, 49, 165–172. [Google Scholar] [CrossRef]
- Otsuka, K.; Koana, T.; Tomita, M.; Ogata, H.; Tauchi, H. Rapid myloid recovery as a possible mechanism of whole-body radioadaptive response. Radiat. Res. 2008, 170, 307–315. [Google Scholar] [CrossRef]
- Monsieurs, M.A.; Thierens, H.M.; Vral, A.M.; Van de Wiele, C.; de Ridder, L.I.; Dierckx, R.A. Adaptive response in patients treated with 131I. J. Nucl. Med. 2000, 41, 17–22. [Google Scholar]
- Vares, G.; Wang, B.; Tanaka, K.; Kakimoto, A.; Eguchi-Kasai, K.; Nenoi, M. Mutagenic adaptive response to high-LET radiation in human lymphoblastoid cells exposed to low doses of heavy-ion radiation. Mutat. Res. 2011, 712, 49–54. [Google Scholar] [CrossRef]
- Nagasawa, H.; Little, J.B. Indcution of sister chromatid exchanges by extremely low doses of -particles. Cancer Res. 1992, 52, 6394–6396. [Google Scholar] [PubMed]
- Suzuki, M.; Yasuda, N.; Kitamura, H. Lethal and mutagenic bystander effects in human fibroblast cell cultures subjected to low-energy-carbon ions. Int. J. Radiat. Biol. 2019, 96, 179–186. [Google Scholar] [CrossRef] [PubMed]
- Konishi, T.; Oikawa, M.; Suya, N.; Ishikawa, T.; Maeda, T.; Kobayashi, A.; Shiomi, N.; Kodama, K.; Hamano, T.; Hamano-Takeda, S.; et al. SPICE-NIRS microbeam: A focused vertical system for proton irradiation of a single cell for radiobiological research. J. Radiat. Res. 2013, 54, 736–747. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Suzuki, M.; Watanabe, M.; Kanai, T.; Kase, Y.; Yatagai, F.; Kato, T.; Matsubara, S. LET dependence of cell death, mutation induction and chromatin damage in human cells irradiated with accelerated carbon ions. Adv. Space Res. 1996, 18, 127–136. [Google Scholar] [CrossRef]
- Suzuki, M.; Tsuruoka, C.; Kanai, T.; Kato, T.; Yatagai, F.; Watanabe, M. Cellular and molecular effects for mutation induction in normal human cells irradiated with accelerated neon ions. Mutat. Res. 2006, 594, 86–92. [Google Scholar] [CrossRef] [PubMed]
- Choi, V.W.Y.; Konishi, T.; Oikawa, M.; Iso, H.; Cheng, S.H.; Yu, K.N. Adaptive response in Zebrafish embryos induced using microbeam protons as priming dose and X-ray photons as challenging dose. J. Radiat. Res. 2010, 51, 657–664. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Suzuki, M.; Zhou, H.; Geard, C.R.; Hei, T.K. Effect of medium on chromatin damage in bystander mammalian cells. Radiat. Res. 2004, 162, 264–269. [Google Scholar] [CrossRef]
Pretreatment Radiation Types | Energy (MeV/n) | LET (keV/µm) | Fluence at 0.1 cGy (ions/cm2) | Average Number of Hits/Nucleus a (%) |
---|---|---|---|---|
Helium ions | 150 | 2.3 | 2.71 × 105 | 61 |
Carbon ions | 290 | 13.3 | 4.69 × 104 | 15 |
Iron ions | 500 | 200 | 3.12 × 103 | 1.1 |
Pretreatment Radiation Types (0.1 cGy/7–8 h) | Cell Survival (%) By X-ray-Challenging Dose (1.5 Gy) a |
---|---|
X-ray-challenging dose 1.5 Gy alone | 39.8 ± 1.6 |
137Cs gamma rays | 34.8 ± 1.9 |
241Am–Be neutrons | 35.7 ± 1.8 |
Helium ions (LET = 2.3 keV/µm) | 41.1 ± 1.7 |
Carbon ions (LET = 13.3 keV/µm) | 40.2 ± 1.8 |
Iron ions (LET = 200 keV/µm) | 42.4 ± 1.3 |
Pretreatment Radiation Types (0.1 cGy/7–8 h) | Induced Mutation Frequency (×10−6) By X-ray-Challenging Dose (1.5 Gy) a |
---|---|
X-ray-challenging dose 1.5 Gy alone | 25.7 ± 5.8 |
137Cs gamma rays | 24.4 ± 5.6 |
241Am–Be neutrons | 6.8 ± 4.0 |
Helium ions (LET = 2.3 keV/µm) | 46.8 ± 6.3 |
Carbon ions (LET = 13.3 keV/µm) | 90.8 ± 7.6 |
Iron ions (LET = 200 keV/µm) | 21.8 ± 5.5 |
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Suzuki, M.; Uchihori, Y.; Kitamura, H.; Oikawa, M.; Konishi, T. Biologic Impact of Different Ultra-Low-Fluence Irradiations in Human Fibroblasts. Life 2020, 10, 154. https://doi.org/10.3390/life10080154
Suzuki M, Uchihori Y, Kitamura H, Oikawa M, Konishi T. Biologic Impact of Different Ultra-Low-Fluence Irradiations in Human Fibroblasts. Life. 2020; 10(8):154. https://doi.org/10.3390/life10080154
Chicago/Turabian StyleSuzuki, Masao, Yukio Uchihori, Hisashi Kitamura, Masakazu Oikawa, and Teruaki Konishi. 2020. "Biologic Impact of Different Ultra-Low-Fluence Irradiations in Human Fibroblasts" Life 10, no. 8: 154. https://doi.org/10.3390/life10080154