Short-Term Sleep Fragmentation Dysregulates Autophagy in a Brain Region-Specific Manner
Abstract
:1. Introduction
2. Methods and Materials
2.1. Animal
2.2. Antibodies and Reagents
2.3. Sleep Fragmentation (SF) Procedure
2.4. Western Blots
2.5. RNA Extraction, Reverse Transcription, and Quantitative Polymerase Chain Reaction (qPCR)
2.6. Statistical Analysis
3. Results
3.1. Short-Term SF Dysregulated Autophagy in the Striatum
3.2. Short-Term SF Dysregulated Autophagy in the Hippocampus
3.3. Short-Term SF Had No Impact on Autophagy in the Frontal Cortex
3.4. Short-Term SF Activated Microglia in a Region-Specific Manner In Vivo
3.5. The Effects of Short-Term SF on Corticotropin Releasing Factor (CRF) Signaling in Extrahypothalamic Regions
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Cheng, Y.; Kim, W.-K.; Wellman, L.L.; Sanford, L.D.; Guo, M.-L. Short-Term Sleep Fragmentation Dysregulates Autophagy in a Brain Region-Specific Manner. Life 2021, 11, 1098. https://doi.org/10.3390/life11101098
Cheng Y, Kim W-K, Wellman LL, Sanford LD, Guo M-L. Short-Term Sleep Fragmentation Dysregulates Autophagy in a Brain Region-Specific Manner. Life. 2021; 11(10):1098. https://doi.org/10.3390/life11101098
Chicago/Turabian StyleCheng, Yan, Woong-Ki Kim, Laurie L. Wellman, Larry D. Sanford, and Ming-Lei Guo. 2021. "Short-Term Sleep Fragmentation Dysregulates Autophagy in a Brain Region-Specific Manner" Life 11, no. 10: 1098. https://doi.org/10.3390/life11101098