Detection of pks Island mRNAs Using Toehold Sensors in Escherichia coli
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plasmid Construction and E. coli Strains
2.2. Cell Culture and Induction Condition
2.3. Fluorescence Measurements Using Flow Cytometry
2.4. Quantitative Reverse-Transcription PCR
2.5. In Silico Toehold Switch Sensor Design for clb ORFs
3. Results
3.1. Helper-Assisted mRNA Sensing of Toehold Switch
3.2. Automated Toehold Switch Design for clb ORFs in the pks Island
3.3. In Vivo Sensing of clb Genes Using Toehold Switch Sensors
3.4. HAM System for the clb ORFs Targeting Toehold Switch Sensors
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Heo, T.; Kang, H.; Choi, S.; Kim, J. Detection of pks Island mRNAs Using Toehold Sensors in Escherichia coli. Life 2021, 11, 1280. https://doi.org/10.3390/life11111280
Heo T, Kang H, Choi S, Kim J. Detection of pks Island mRNAs Using Toehold Sensors in Escherichia coli. Life. 2021; 11(11):1280. https://doi.org/10.3390/life11111280
Chicago/Turabian StyleHeo, Taeyang, Hansol Kang, Seungdo Choi, and Jongmin Kim. 2021. "Detection of pks Island mRNAs Using Toehold Sensors in Escherichia coli" Life 11, no. 11: 1280. https://doi.org/10.3390/life11111280