Pheophorbide a Derivatives Exert Antiwrinkle Effects on UVB-Induced Skin Aging in Human Fibroblasts
Abstract
:1. Introduction
2. Results
2.1. Effects of Pheophorbides on the Viability of CCD-986sk Cells
2.2. Effects of Pheophorbides on ROS Accumulation in CCD-986sk Cells
2.3. Effects of Pheophorbides on MMP Expression in CCD-986sk Cells
2.4. Effects of Pheophorbides on Collagen Expression in CCD-986sk Cells
2.5. Effects of Pheophorbides on MAPK and NF-κB Signaling in CCD-986sk Cells
3. Discussion
4. Materials and Methods
4.1. Chemicals
4.2. Cell Culture and Cell Viability Assay
4.3. Measurement of ROS Accumulation
4.4. Detection of MMP-1 and Procollagen Concentration
4.5. Real-Time Quantitative RT-PCR (qRT-PCR)
4.6. Immunoblotting
4.7. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Gene (NCBI Reference Sequence) | Forward Primer | Reverse Primer |
---|---|---|
ACTIN (NM_001101) | GGCACCACACCTTCTACAAT | GCCTGGATAGCAACGTACAT |
COL1A1 (NM_000088) | TGGCCTCGGAGGAAACTTT | GCTTCCCCATCATCTCCATTC |
COL1A2 (NM_000089) | CGGTGGTGGTTATGACTTTGGT | GAAGGGTCTCAATCTGGTTGTTG |
MMP-1 (NM_001145938) | AACACATCTGACCTACAGGATTGAAA | CTTGGTGAATGTCAGAGGTGTGA |
MMP-2 (NM_001127891) | ACTGGAGCAAAAACAAGAAGACATAC | TCCATTTTCTTCTTCACCTCATTG |
MMP-9 (NM_004994) | GCGCTGGGCTTAGATCATTC | GTGCCGGATGCCATTCA |
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Lee, H.; Park, H.-Y.; Jeong, T.-S. Pheophorbide a Derivatives Exert Antiwrinkle Effects on UVB-Induced Skin Aging in Human Fibroblasts. Life 2021, 11, 147. https://doi.org/10.3390/life11020147
Lee H, Park H-Y, Jeong T-S. Pheophorbide a Derivatives Exert Antiwrinkle Effects on UVB-Induced Skin Aging in Human Fibroblasts. Life. 2021; 11(2):147. https://doi.org/10.3390/life11020147
Chicago/Turabian StyleLee, Hwa, Ho-Yong Park, and Tae-Sook Jeong. 2021. "Pheophorbide a Derivatives Exert Antiwrinkle Effects on UVB-Induced Skin Aging in Human Fibroblasts" Life 11, no. 2: 147. https://doi.org/10.3390/life11020147