The Effect of a Total Fishmeal Replacement by Arthrospira platensis on the Microbiome of African Catfish (Clarias gariepinus)
Abstract
:1. Introduction
2. Materials and Methods
2.1. Fish and Rearing Conditions
2.2. Microbiome
2.2.1. Sampling
2.2.2. DNA Extraction
2.2.3. 16S rRNA Gene Amplification and Sequencing
2.2.4. Sequence Processing and Analyses
2.3. Data Processing
2.4. Sequence Data Deposition
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Ingredient (% Dry Matter) | FM100 | SP100 |
---|---|---|
Fishmeal 1 | 20.00 | 0.00 |
Spirulina 2 | 0.00 | 20.00 |
Fish oil | 10.70 | 10.70 |
Wheat meal | 14.00 | 12.50 |
Wheat gluten | 20.00 | 21.50 |
Soy protein concentrate 3 | 20.00 | 20.00 |
Rapeseed oil | 10.70 | 10.70 |
Vit./Min. Premix | 1.00 | 1.00 |
CaHPO4 | 1.00 | 1.00 |
CMC (Binder) | 1.29 | 1.08 |
TiO2 (Marker) | 0.50 | 0.50 |
Fe3O4–black (Dye) | 0.07 | 0.07 |
L-Lysin (HCL-Lys, 78% Lys) | 0.70 | 0.90 |
D,L-Methionine | 0.01 | 0.04 |
L-Tryptophan | 0.03 | 0.01 |
Approximate Composition (%) | FM100 | SP100 |
---|---|---|
Dry matter | 94.6 | 94.0 |
Crude protein (N × 6.25) | 45.4 | 45.7 |
Crude lipids | 24.6 | 23.9 |
N-free extracts | 17.5 | 19.0 |
Crude ash | 7.1 | 5.4 |
Gross energy [MJ/kg] | 23.4 | 23.5 |
Digestible energy [MJ/kg] | 20.0 | 20.0 |
Essential amino acids 1 | 26.6 | 26.8 |
Genus | FM100 | SP100 |
---|---|---|
Cetobacterium | 54.98 ± 20.43 | 58.90 ± 13.34 |
Romboutsia | 20.30 ± 14.88 | 8.14 ± 3.26 |
Macellibacteroides | 4.15 ± 4.10 | 5.05 ± 2.50 |
Plesiomonas | 2.95 ± 2.47 | 5.66 ± 2.95 |
Bacteroides | 1.50 ± 0.61 | 8.82 ± 5.72 |
[Anaerorhabdus] furcosa group | 3.44 ± 2.96 | 1.88 ± 1.92 |
[Barnesiellaceae] uncultured | 1.30 ± 1.15 | 2.17 ± 1.72 |
Clostridium sensu stricto 1 | 2.39 ± 1.94 | 0.72 ± 0.38 |
Turicibacter | 2.25 ± 2.30 | 0.21 ± 0.19 |
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Rosenau, S.; Oertel, E.; Mott, A.C.; Tetens, J. The Effect of a Total Fishmeal Replacement by Arthrospira platensis on the Microbiome of African Catfish (Clarias gariepinus). Life 2021, 11, 558. https://doi.org/10.3390/life11060558
Rosenau S, Oertel E, Mott AC, Tetens J. The Effect of a Total Fishmeal Replacement by Arthrospira platensis on the Microbiome of African Catfish (Clarias gariepinus). Life. 2021; 11(6):558. https://doi.org/10.3390/life11060558
Chicago/Turabian StyleRosenau, Simon, Elisa Oertel, Alexander Charles Mott, and Jens Tetens. 2021. "The Effect of a Total Fishmeal Replacement by Arthrospira platensis on the Microbiome of African Catfish (Clarias gariepinus)" Life 11, no. 6: 558. https://doi.org/10.3390/life11060558