Overexpression of p75NTR in Human Seminoma: A New Biomarker?
Abstract
:1. Introduction
2. Materials and Methods
2.1. Antibodies
2.2. Human Tissues
2.3. Tissue Microarray (TMA) Construction and Validation
2.4. Histopathological Analysis
2.5. Immunohistochemistry
2.6. Scoring System
2.7. Statistical Analysis
3. Results
3.1. Morphology Analysis in Control Testis and Seminoma
3.2. Immunohistochemical Localization of p75NTR, NGF and TrkA in Control Testis and TGCC
3.3. Immunohistochemistry of p53 in TGCC
3.4. Validation of TMA Method
3.5. Clinico-Pathological Significance of p75NTR, p-JNK and p53
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Control (n = 4) | Seminoma (n = 26) | |
---|---|---|
p75NTR | 4 (3–4) | 8 (7–8) *** |
NGF | 2 (2–3) | 0 (0–0) |
TrkA | 3 (2–4) | 0 (0–0) |
Marker | Cutoff | Sensitivity (%) | Specificity (%) | PPV | NPV | AUC (95% CI) |
---|---|---|---|---|---|---|
p75NTR | ≥7 | 67.8% | 59.0% | 21.4% | 76.0% | 0.80 (0.75–0.98) |
Marker | Control (n = 4) | Seminoma (n = 26) |
---|---|---|
p-JNK | 3 (2–3) | 7 (6–8) *** |
P53 | 3 (2–3) | 7 (6–8) *** |
nr | NGF | TrkA | p75NTR | ||||
---|---|---|---|---|---|---|---|
Positive (%) | p | Positive (%) | p | Positive (%) | p | ||
T stage | 0.91 | 0.40 | 0.001 | ||||
T1 | 27 | 1 (0.03) | 5 (5.9) | 21 (77.7) | |||
T2 | 7 | 0 (0.0) | 0 (0.0) | 2 (28.5) | |||
T3 | 5 | 0 (0.0) | 0 (0.0) | 1 (20.0) | |||
T4 | 1 | 0 (0.0) | 0 (0.0) | 0 (0.0) | |||
Vascular invasion | ns | ns | 0.59 | ||||
No | 34 | 0 (0.0) | 0 (0.0) | 13 (38.2) | |||
Yes | 6 | 0 (0.0) | 0 (0.0) | 3 (64.3) | |||
Histology | ns | ns | 0.0001 | ||||
Seminoma | 26 | 0 (0.0) | 0 (0.0) | 25 (96.1) | |||
Non-seminomatous | 14 | 0 (0.0) | 0 (0.0) | 4 (28.5) |
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Perri, A.; Rago, V.; Malivindi, R.; Maltese, L.; Lofaro, D.; Greco, E.A.; Tucci, L.; Bonofiglio, R.; Vergine, M.; La Vignera, S.; et al. Overexpression of p75NTR in Human Seminoma: A New Biomarker? Life 2021, 11, 629. https://doi.org/10.3390/life11070629
Perri A, Rago V, Malivindi R, Maltese L, Lofaro D, Greco EA, Tucci L, Bonofiglio R, Vergine M, La Vignera S, et al. Overexpression of p75NTR in Human Seminoma: A New Biomarker? Life. 2021; 11(7):629. https://doi.org/10.3390/life11070629
Chicago/Turabian StylePerri, Anna, Vittoria Rago, Rocco Malivindi, Lorenza Maltese, Danilo Lofaro, Emanuela Alessandra Greco, Luigi Tucci, Renzo Bonofiglio, Margherita Vergine, Sandro La Vignera, and et al. 2021. "Overexpression of p75NTR in Human Seminoma: A New Biomarker?" Life 11, no. 7: 629. https://doi.org/10.3390/life11070629