Sepsityper® Kit versus In-House Method in Rapid Identification of Bacteria from Positive Blood Cultures by MALDI-TOF Mass Spectrometry
Abstract
:1. Introduction
2. Materials and Methods
3. Results
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Conflicts of Interest
References
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Microorganisms as Identified by the Routine Method | N | In-House Method | Sepsityper Kit | ||||
---|---|---|---|---|---|---|---|
<1.700 | 1.700–1.999 | ≥2.000 | <1.600 | 1.600–1.799 | ≥1.800 | ||
Gram-negative bacteria | 53 | 2 | 7 | 44 | 3 | 3 | 47 |
Acinetobacter baumannii | 7 | 1 | 6 | 1 | 1 | 5 | |
Bacteroides fragilis | 1 | 1 | 1 | ||||
Enterobacter asburiae | 1 | 1 | 1 | ||||
Enterobacter cloacae | 2 | 2 | 2 | ||||
Escherichia coli | 10 | 2 | 8 | 1 | 9 | ||
Klebsiella aerogenes | 1 | 1 | 1 | ||||
Klebsiella oxytoca | 1 | 1 | 1 | ||||
Klebsiella pneumoniae | 12 | 1 | 1 | 10 | 1 | 1 | 10 |
Providentia stuartii | 1 | 1 | 1 | ||||
Pseudomonas aeruginosa | 8 | 8 | 8 | ||||
Pseudomonas stutzeri | 1 | 1 | 1 | ||||
Salmonella sp | 1 | 1 | 1 | ||||
Serratia marcescens | 5 | 1 | 4 | 5 | |||
Stenotrophomonas maltophilia | 2 | 1 | 1 | 2 | |||
Gram-positive bacteria | 60 | 22 | 20 | 18 | 11 | 9 | 40 |
Corynebacterium striatum | 1 | 1 | 1 | ||||
Dietzia papillomatosis | 1 | 1 | 1 | ||||
Enterococcus faecalis | 2 | 1 | 1 | 2 | |||
Enterococcus faecium | 6 | 1 | 5 | 6 | |||
Micrococcus luteus1 | 1 | ||||||
Parvimonas micra1 | 1 | ||||||
Staphylococcus aureus | 9 | 5 | 2 | 2 | 2 | 7 | |
Staphylococcus capitis | 2 | 1 | 1 | 2 | |||
Staphylococcus cohnii | 1 | 1 | 1 | ||||
Staphylococcus epidermidis | 18 | 8 | 8 | 2 | 4 | 4 | 10 |
Staphylococcus haemolyticus | 12 | 3 | 6 | 3 | 1 | 4 | 7 |
Staphylococcus hominis | 3 | 3 | 3 | ||||
Staphylococcus lungdunensis | 1 | 1 | 1 | ||||
Streptococcus anginosus | 1 | 1 | 1 | ||||
Streptococcus gordonii | 1 | 1 | 1 | ||||
Total | 113 | 24 | 27 | 62 | 14 | 12 | 87 |
Microorganism Group | In-House Method | Sepsityper® Kit | In-House Method Versus Sepsityper® Kit | |||
---|---|---|---|---|---|---|
Identified to the Genus Level (Score ≥ 1.700, %) | Identified to the Species Level (Score ≥ 2.000, %) | Identified to the Genus Level (Score ≥ 1.600, %) | Identified to the Species Level (Score ≥ 1.800, %) | p-Value (Genus Level Identification) | p-Value (Species Level Identification) | |
Gram-negative bacteria | 96.2 | 83.0 | 94.3 | 88.7 | 1.000 | 0.405 |
Gram-positive bacteria | 63.3 | 30.0 | 81.7 | 66.7 | 0.025 | <0.001 |
Total | 78.8 | 54.9 | 87.6 | 77.0 | 0.076 | 0.001 |
Microorganism Group | Without Additional On-Plate Formic Acid N (%) | With Additional On-Plate Formic Acid N (%) | In-House Method without Versus with Formic Acid | |||
---|---|---|---|---|---|---|
Genus Level ≥ 1.700 | Species Level ≥ 2.000 | Genus Level ≥ 1.700 | Species Level ≥ 2.000 | p-Value (Genus Identification | p-Value (Species Identification) | |
Gram-negative bacteria (n = 53) | 47 (88.7) | 36 (67.9) | 49 (92.5) | 41 (77.4) | 0.742 | 0.278 |
Gram-positive bacteria (n = 60) | 5 (8.3) | 3 (5.0) | 38 (63.3) | 18 (30.0) | <0.001 | 0.001 |
Total (n = 113) | 52 (46.0) | 39 (34.5) | 87 (77.0) | 59 (52.2) | <0.001 | 0.007 |
Microorganism Group | Without AdditionalOn-Plate Formic Acid N (%) | With Additional On-Plate Formic Acid N (%) | Sepsityper® Kit without Versus with Formic Acid | |||
---|---|---|---|---|---|---|
Genus Level ≥ 1.600 | Species Level ≥ 1.800 | Genus Level ≥ 1.600 | Species Level ≥ 1.800 | p-Value (Genus Identification | p-Value (Species Identification) | |
Gram-negative bacteria (n = 53) | 46 (86.8) | 44 (83.0) | 48 (90.6) | 43 (81.1) | 0.761 | 0.801 |
Gram-positive bacteria (n = 60) | 33 (55.0) | 19 (31.7) | 47 (78.3) | 39 (65.0) | 0.007 | <0.001 |
Total (n = 113) | 79 (69.9) | 63 (55.8) | 95 (84.1) | 82 (72.6) | 0.012 | 0.009 |
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Perše, G.; Samošćanec, I.; Bošnjak, Z.; Budimir, A.; Kuliš, T.; Mareković, I. Sepsityper® Kit versus In-House Method in Rapid Identification of Bacteria from Positive Blood Cultures by MALDI-TOF Mass Spectrometry. Life 2022, 12, 1744. https://doi.org/10.3390/life12111744
Perše G, Samošćanec I, Bošnjak Z, Budimir A, Kuliš T, Mareković I. Sepsityper® Kit versus In-House Method in Rapid Identification of Bacteria from Positive Blood Cultures by MALDI-TOF Mass Spectrometry. Life. 2022; 12(11):1744. https://doi.org/10.3390/life12111744
Chicago/Turabian StylePerše, Gabrijela, Ivana Samošćanec, Zrinka Bošnjak, Ana Budimir, Tomislav Kuliš, and Ivana Mareković. 2022. "Sepsityper® Kit versus In-House Method in Rapid Identification of Bacteria from Positive Blood Cultures by MALDI-TOF Mass Spectrometry" Life 12, no. 11: 1744. https://doi.org/10.3390/life12111744