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Volume 12
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10.3390/life12020304
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Article

The Blood of the HIV-Infected Patients Contains κ-IgG, λ-IgG, and Bispecific κλ-IgG, Which Possess DNase and Amylolytic Activity

by
Anna Timofeeva
1,*,†,
Sergey Sedykh
1,2,†,
Lada Maksimenko
3,
Tatyana Sedykh
1,
Sergey Skudarnov
4,
Tatyana Ostapova
4,
Svetlana Yaschenko
4,
Natalya Gashnikova
3 and
Georgy Nevinsky
1,2
1
SB RAS Institute of Chemical Biology and Fundamental Medicine, 630090 Novosibirsk, Russia
2
Faculty of Natural Sciences, Novosibirsk State University, 630090 Novosibirsk, Russia
3
State Research Center of Virology and Biotechnology Vector, 630559 Koltsovo, Russia
4
Krasnoyarsk Regional Center for Prevention and Control of AIDS, 660049 Krasnoyarsk, Russia
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Life 2022, 12(2), 304; https://doi.org/10.3390/life12020304
Submission received: 10 January 2022 / Revised: 4 February 2022 / Accepted: 14 February 2022 / Published: 17 February 2022
(This article belongs to the Special Issue Bispecific Antibodies: Design, Isolation, Perspectives of Use)
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Abstract

Though hundreds of thousands of papers are currently being published on HIV/AIDS, only tens of hundreds of them are devoted to the antibodies generated during the disease. Most of these papers discuss antibodies in HIV/AIDS as a diagnostic tool, and some articles describe neutralizing antibodies as a promising treatment. In this paper, we used affinity chromatography and ELISA to isolate natural IgG from the blood of 26 HIV-infected patients. IgG preparations were separated into the subfractions containing different types of light chains, and catalytic activities of subfractions were analyzed. Here, we show for the first time that the blood of HIV patients contains ~20% of bispecific κλ-IgG, presented with all IgG subclasses. Analysis of DNA-hydrolyzing and amylolytic activity show that most IgG preparations and subfractions are catalytically active. Our results expand the possible biological functions of natural IgG in HIV infection.
Keywords: HIV; bispecific antibodies; IgG; immunoglobulin G; catalytic activity; abzymes; DNAse activity; DNA hydrolysis; amylolytic activity; ELISA HIV; bispecific antibodies; IgG; immunoglobulin G; catalytic activity; abzymes; DNAse activity; DNA hydrolysis; amylolytic activity; ELISA

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MDPI and ACS Style

Timofeeva, A.; Sedykh, S.; Maksimenko, L.; Sedykh, T.; Skudarnov, S.; Ostapova, T.; Yaschenko, S.; Gashnikova, N.; Nevinsky, G. The Blood of the HIV-Infected Patients Contains κ-IgG, λ-IgG, and Bispecific κλ-IgG, Which Possess DNase and Amylolytic Activity. Life 2022, 12, 304. https://doi.org/10.3390/life12020304

AMA Style

Timofeeva A, Sedykh S, Maksimenko L, Sedykh T, Skudarnov S, Ostapova T, Yaschenko S, Gashnikova N, Nevinsky G. The Blood of the HIV-Infected Patients Contains κ-IgG, λ-IgG, and Bispecific κλ-IgG, Which Possess DNase and Amylolytic Activity. Life. 2022; 12(2):304. https://doi.org/10.3390/life12020304

Chicago/Turabian Style

Timofeeva, Anna, Sergey Sedykh, Lada Maksimenko, Tatyana Sedykh, Sergey Skudarnov, Tatyana Ostapova, Svetlana Yaschenko, Natalya Gashnikova, and Georgy Nevinsky. 2022. "The Blood of the HIV-Infected Patients Contains κ-IgG, λ-IgG, and Bispecific κλ-IgG, Which Possess DNase and Amylolytic Activity" Life 12, no. 2: 304. https://doi.org/10.3390/life12020304

APA Style

Timofeeva, A., Sedykh, S., Maksimenko, L., Sedykh, T., Skudarnov, S., Ostapova, T., Yaschenko, S., Gashnikova, N., & Nevinsky, G. (2022). The Blood of the HIV-Infected Patients Contains κ-IgG, λ-IgG, and Bispecific κλ-IgG, Which Possess DNase and Amylolytic Activity. Life, 12(2), 304. https://doi.org/10.3390/life12020304

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