Study on the Preparation and Effect of Tomato Seedling Disease Biocontrol Compound Seed-Coating Agent
Abstract
:1. Introduction
2. Materials and Methods
2.1. Experimental Strains and Plant Materials
2.2. Inhibitory Effect of Biocontrol Strains on PA-4 and FS-1
2.3. Germination-Promoting Effect of Five Isolates and Compound Bacteria on Tomato Seeds
2.4. Screening of Five Biocontrol Bacterial Composite Proportions
2.5. Effects of Different Auxiliaries on the Activity of Five Microorganisms
2.6. S-Compound Microbial Seed Coating Formulation
2.7. Optimization of S-Compound Microbial Seed Coating Formulation
2.8. Growth Promotion of Tomato Seedlings by S-Composite Microbial Seed Coating
2.9. Control Effect of S-Composite Microbial Seed Coating on Tomato Damping-Off and Root Rot Diseases
3. Results
3.1. Inhibitory Effect of Five Biocontrol Bacteria on sPA-4 and FS-1
3.2. Growth-Promoting Effect of Five Biocontrol Bacteria on Tomato Seeds
3.3. Screening of the Ratio of Five Biocontrol Bacteria
3.4. Selection of S-Compound Microbial Seed-Coating Agent
3.5. Development of S-Compound Microbial Seed Coating Formulation
3.6. Optimization of S-Compound Microbial Seed Coating Formulation
3.7. Promoting Effect of S-Complex Microbial Seed Coating on Tomato Seedlings
3.8. Preventive Effect of S-Complex Microbial Seed Coating on Tomato Damping-Off and Root Rot Disease
3.9. S-Composite Microbial Seed-Coating Agent Shelf Life Testing
4. Discussion
5. Conclusions
- The five biocontrol bacteria promote the germination of tomato seeds and the growth of radicles. The combination of WZ-37, WXCDD105, WXCDD51, BS wy-1, and Ba in the ratio of 100:100:1:1 could increase the germination rate of seeds by 4.5% in 48 h.
- Five additives with little damage to microbial activity were selected, including gas-phase silica, bentonite, diatomite, gum arabic, and sodium lignosulfonate. The preliminary formula of seed-coating agent was determined by orthogonal tests, and the final additives of seed-coating agent were gas-phase silica, gum arabic, and sodium dodecylbenzene sulfonate.
- Upon addition of 33.3 μL GA3 to S-compound microbial seed-coating agent, the effect of improving seed germination rate was the most significant.
- S-compound microbial seed-coating agent had significant growth-promoting effects on tomato seeds and seedlings. Pot experiments proved that S-compound microbial seed coating had good preventive effect on tomato damping-off disease and root rot, which was better than commercial chemical pesticide carbendazim wettable powder.
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Biocontrol Strains | PA-4 (Pythium aphanidermatum) | FS-1 (Fusarium sp.) | |
---|---|---|---|
WZ-37 (Bacillus velezensis) | Dry weight of mycelium (g) | 0.015 ± 0.0002c | 0.000 ± 0.0000f |
Inhibition rate (g) | 92.96% | 100.00% | |
WXCDD105 (Bacillus subtilis) | Dry weight of mycelium (g) | 0.018 ± 0.0005b | 0.012 ± 0.0005d |
Inhibition rate (g) | 91.55% | 96.67% | |
WXCDD51 (Pseudomonas fluorescens) | Dry weight of mycelium (g) | 0.000 ± 0.0000f | 0.016 ± 0.0007c |
Inhibition rate (g) | 100.00% | 95.54% | |
Bs wy-1 (Bacillus subtilis) | Dry weight of mycelium (g) | 0.010 ± 0.0003e | 0.019 ± 0.0002b |
Inhibition rate (g) | 91.08% | 94.71% | |
Ba (Bacillus amyloliquefaciens) | Dry weight of mycelium (g) | 0.013 ± 0.0002d | 0.01 ± 0.0005e |
Inhibition rate (g) | 93.90% | 97.21% | |
H (bacterial mixture) | Dry weight of mycelium (g) | 0.000 ± 0.0000f | 0.000 ± 0.0000f |
Inhibition rate (g) | 100.00% | 100.00% | |
CK (sterile water) | Dry weight of mycelium (g) | 0.213 ± 0.0008a | 0.359 ± 0.0004a |
Inhibition rate (g) | - | - |
Treatment | Plant Height/cm | Stem Diameter/mm | Root Length/cm | Fresh Weight/g | Dry Weight/g |
---|---|---|---|---|---|
CK | 7.3 ± 0.70b | 1.42 ± 0.03b | 4.33 ± 0.23b | 1.20 ± 0.12c | 0.081 ± 0.01b |
D | 7.43 ± 0.35b | 1.41 ± 0.13b | 5.10 ± 0.53ab | 1.51 ± 0.06b | 0.0903 ± 0.01b |
S | 9.95 ± 0.73a | 1.88 ± 0.15a | 6.01 ± 0.28a | 2.71 ± 0.08a | 0.1727 ± 0.01a |
Treatment | Disease Index/100% | Relative Control Effect/100% |
---|---|---|
CK | 52.38 ± 6.44a | |
S | 8.07 ± 3.59c | 84.54 |
D | 31.43 ± 7.42b | 40 |
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Zhang, Y.; Li, Y.; Liang, S.; Zheng, W.; Chen, X.; Liu, J.; Wang, A. Study on the Preparation and Effect of Tomato Seedling Disease Biocontrol Compound Seed-Coating Agent. Life 2022, 12, 849. https://doi.org/10.3390/life12060849
Zhang Y, Li Y, Liang S, Zheng W, Chen X, Liu J, Wang A. Study on the Preparation and Effect of Tomato Seedling Disease Biocontrol Compound Seed-Coating Agent. Life. 2022; 12(6):849. https://doi.org/10.3390/life12060849
Chicago/Turabian StyleZhang, Yao, Yingying Li, Sibo Liang, Wei Zheng, Xiuling Chen, Jiayin Liu, and Aoxue Wang. 2022. "Study on the Preparation and Effect of Tomato Seedling Disease Biocontrol Compound Seed-Coating Agent" Life 12, no. 6: 849. https://doi.org/10.3390/life12060849