Comparative Transcriptome Profiling Provides Insights into Plant Salt Tolerance in Watermelon (Citrullus lanatus)
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials and Treatments
2.2. Measurements of Physiological and Biochemical Index
2.3. RNA Extraction, Sequencing and Expression Profiling
2.4. RT-qPCR for DEGs
3. Results
3.1. Salt Stress Inhibits the Growth of Watermelon Seedlings
3.2. RNA-Seq for the Roots and Leaves of Watermelon under Salt Treatment
3.3. Verification of DEGs by RT-qPCR
3.4. GO Classification and KEGG Pathway Enrichment Analysis of DEGs
3.5. Transcription Factors Respond to Salt Stress
3.6. DEGs in Response to Salt Stress
3.7. Key DEGs Related to Salt Stress in Watermelon
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
RWC | relative water content |
MDA | malondialdehyde acid |
ROS | reactive oxygen species |
NAA | naphthaleneacetic acid |
JA | jasmonic acid |
IAA | indole-3-acetic acid |
GA | gibberellin |
SA | salicylic acid |
DEGs | differentially expressed genes |
FDR | false discovery rate |
ORF | open reading frame |
TPS | trehalose-6-phosphate synthase |
HKT | high-affinity K+ transporter |
KUP | K+ uptake permease |
PCA | principal component analysis |
HSP | heat shock protein |
CYP | cytochrome P450 |
SAUR | small auxin up RNA |
GH3 | gretchen hagen 3 |
ARF | auxin response factor |
CESAs | cellulose synthases |
PEPCK | phosphoenolpyruvate carboxykinase |
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Tissue | Gene ID | KEGG Number | Log2 (TT/TC) | Log2 (ST/SC) | Nr Description |
---|---|---|---|---|---|
Root | Cla97C03G054690 | ko00620 | 1.85 | 6.31 | malate synthase |
Cla97C05G107020 | ko00620 | −1.46 | −1.25 | pyruvate dehydrogenase E1 | |
Cla97C05G107020 | ko00020 | −1.46 | −1.25 | pyruvate dehydrogenase E1 | |
Novel_G000429 | ko00052 | −1.53 | −2.74 | UDP-sugar pyrophosphorylase | |
Cla97C01G009200 | ko00052 | 0.78 | 1.19 | alpha-glucosidase | |
Cla97C02G031490 | ko00052 | −2.47 | −2.20 | alcohol dehydrogenase (NADP+) | |
Cla97C10G197800 | ko00052 | 1.37 | 1.96 | aldose 1-epimerase | |
Cla97C10G197810 | ko00052 | 1.37 | 1.96 | alcohol dehydrogenase (NADP+) | |
Cla97C02G031490 | ko00010 | −2.47 | −2.20 | alcohol dehydrogenase (NADP+) | |
Cla97C05G107020 | ko00010 | −1.46 | −1.25 | pyruvate dehydrogenase E1 | |
Cla97C10G197800 | ko00010 | 1.37 | 1.96 | aldose 1-epimerase | |
Cla97C10G197810 | ko00010 | 1.37 | 1.96 | aldose 1-epimerase | |
Cla97C11G206680 | ko00780 | 2.43 | 3.67 | NADPH-dependent pterin aldehyde reductase-like | |
Leaf | Cla97C10G192070 | ko00620 | 0.45 | 1.35 | phosphoenolpyruvate carboxykinase [ATP] |
Cla97C11G216650 | ko00620 | −1.57 | −1.64 | lactoylglutathione lyase | |
Cla97C11G220850 | ko00620 | 1.00 | 3.73 | phosphoenolpyruvate carboxykinase [ATP] | |
Cla97C10G192070 | ko00020 | 0.45 | 1.35 | phosphoenolpyruvate carboxykinase [ATP] | |
Cla97C11G220850 | ko00020 | 1.00 | 3.73 | phosphoenolpyruvate carboxykinase [ATP] | |
Cla97C07G140230 | ko00052 | −0.58 | −0.83 | ATP-dependent 6-phosphofructokinase 6 | |
Cla97C08G156510 | ko00052 | −1.18 | −1.42 | beta-fructofuranosidase | |
Cla97C08G156670 | ko00052 | −1.31 | −1.47 | beta-fructofuranosidase | |
Cla97C07G140230 | ko00010 | −0.58 | −0.83 | ATP-dependent 6-phosphofructokinase 6 | |
Cla97C10G192070 | ko00010 | 0.45 | 1.35 | phosphoenolpyruvate carboxykinase [ATP] | |
Cla97C11G220850 | ko00010 | 1.00 | 3.73 | phosphoenolpyruvate carboxykinase [ATP] | |
Cla97C11G206570 | ko00780 | −0.47 | −0.93 | NADPH-dependent pterin aldehyde reductase |
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Zhu, Y.; Yuan, G.; Gao, B.; An, G.; Li, W.; Si, W.; Sun, D.; Liu, J. Comparative Transcriptome Profiling Provides Insights into Plant Salt Tolerance in Watermelon (Citrullus lanatus). Life 2022, 12, 1033. https://doi.org/10.3390/life12071033
Zhu Y, Yuan G, Gao B, An G, Li W, Si W, Sun D, Liu J. Comparative Transcriptome Profiling Provides Insights into Plant Salt Tolerance in Watermelon (Citrullus lanatus). Life. 2022; 12(7):1033. https://doi.org/10.3390/life12071033
Chicago/Turabian StyleZhu, Yingchun, Gaopeng Yuan, Bowen Gao, Guolin An, Weihua Li, Wenjing Si, Dexi Sun, and Junpu Liu. 2022. "Comparative Transcriptome Profiling Provides Insights into Plant Salt Tolerance in Watermelon (Citrullus lanatus)" Life 12, no. 7: 1033. https://doi.org/10.3390/life12071033