IL-18 Signaling Is Essential for Causing Streptococcal Toxic Shock-like Syndrome (STSLS)
Abstract
:1. Introduction
2. Materials and Methods
2.1. S. suis Strain and Culture Conditions
2.2. Ethics Statement
2.3. Construction of il18r1 Gene Knockout Mice
2.4. Experimental Infections of Mice
2.5. Multiplex Cytokine Assays
2.6. Histopathology Examinations
2.7. Bacterial Load in The Blood and Tissues
2.8. Statistical Analysis
3. Results and Discussion
3.1. Construction of il18r1 Gene Knockout Mice
3.2. Knockout of il18r1 Could Significantly Decrease Mortality during STSLS
3.3. Knockout of il18r1 Could Decrease the Tissue Damages during STSLS
3.4. Knockout of il18r1 Decreases Serum Inflammatory Cytokine Storms but Does Not Significantly Decrease Bacterial Load
3.5. Limitation
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
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Xu, L.; Zeng, Y.; Gao, P.; Lu, X.; Xia, K.; Zhou, L.; Zhang, C.; Yi, C.; Zhang, A. IL-18 Signaling Is Essential for Causing Streptococcal Toxic Shock-like Syndrome (STSLS). Life 2022, 12, 1324. https://doi.org/10.3390/life12091324
Xu L, Zeng Y, Gao P, Lu X, Xia K, Zhou L, Zhang C, Yi C, Zhang A. IL-18 Signaling Is Essential for Causing Streptococcal Toxic Shock-like Syndrome (STSLS). Life. 2022; 12(9):1324. https://doi.org/10.3390/life12091324
Chicago/Turabian StyleXu, Lei, Yue Zeng, Peiying Gao, Xi Lu, Kunlong Xia, Liting Zhou, Chengfeng Zhang, Chenyang Yi, and Anding Zhang. 2022. "IL-18 Signaling Is Essential for Causing Streptococcal Toxic Shock-like Syndrome (STSLS)" Life 12, no. 9: 1324. https://doi.org/10.3390/life12091324