Flammulina filiformis Pkac Gene Complementing in Neurospora crassa Mutant Reveals Its Function in Mycelial Growth and Abiotic Stress Response
Abstract
:1. Introduction
2. Materials and Methods
2.1. Strains and Culture Conditions
2.2. Sequence, Structure and Similarity Analysis of Pkac Gene in F. filiformis and N. crassa
2.3. Total DNA and RNA Extraction and RT-qPCR
2.4. Construction of Complementation Vector of N. crassa
2.5. RNA-Seq of N. crassa Transformants
3. Results
3.1. Structure and Protein Function of Pkac Gene
3.2. Expression Pattern of Pkac Gene
3.3. Pkac Gene Regulates Mycelial Growth
3.4. Pkac Gene Participates in Abiotic Stress Response
3.5. Identification and Validation of Downstream Regulatory Genes of Pkac
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Yang, Y.; Xie, B.; Jing, Z.; Lu, Y.; Ye, J.; Chen, Y.; Liu, F.; Li, S.; Xie, B.; Tao, Y. Flammulina filiformis Pkac Gene Complementing in Neurospora crassa Mutant Reveals Its Function in Mycelial Growth and Abiotic Stress Response. Life 2022, 12, 1336. https://doi.org/10.3390/life12091336
Yang Y, Xie B, Jing Z, Lu Y, Ye J, Chen Y, Liu F, Li S, Xie B, Tao Y. Flammulina filiformis Pkac Gene Complementing in Neurospora crassa Mutant Reveals Its Function in Mycelial Growth and Abiotic Stress Response. Life. 2022; 12(9):1336. https://doi.org/10.3390/life12091336
Chicago/Turabian StyleYang, Yayong, Bin Xie, Zhuohan Jing, Yuanping Lu, Jun Ye, Yizhao Chen, Fang Liu, Shaojie Li, Baogui Xie, and Yongxin Tao. 2022. "Flammulina filiformis Pkac Gene Complementing in Neurospora crassa Mutant Reveals Its Function in Mycelial Growth and Abiotic Stress Response" Life 12, no. 9: 1336. https://doi.org/10.3390/life12091336