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Article

Effects of Vertically Heterogeneous Soil Salinity on Genetic Polymorphism and Productivity of the Widespread Halophyte Bassia prostrata

by
Elena Shuyskaya
1,*,
Kristina Toderich
2,
Alexander Kolesnikov
3,
Maria Prokofieva
1 and
Marina Lebedeva
4
1
K.A. Timiryazev Institute of Plant Physiology RAS, 35 Botanicheskaya St., 127276 Moscow, Russia
2
International Platform for Dryland Research and Education, Tottori University, Tottori 680-0001, Japan
3
Institute of Forest Science, Russian Academy of Sciences, 143030 Uspenskoe, Russia
4
V.V. Dokuchaev Soil Science Institute, 7/2 Pyzhevsky per., 119017 Moscow, Russia
*
Author to whom correspondence should be addressed.
Life 2023, 13(1), 56; https://doi.org/10.3390/life13010056
Submission received: 23 November 2022 / Revised: 17 December 2022 / Accepted: 21 December 2022 / Published: 24 December 2022
(This article belongs to the Special Issue Plant Biotic and Abiotic Stresses)

Abstract

:
Salinity is one of the environmental factors that affects both productivity and genetic diversity in plant species. Within the soil profile, salinity is a dynamic indicator and significantly changes with depth. The present study examined the effects of the vertical heterogeneity of soil salinity chemistry on the plant height, fresh and dry biomass accumulation, water content, level of genetic polymorphism, and observed and expected heterozygosity in seven populations of halophyte Bassia prostrata in natural habitats. Soil salinity ranged from slight (Ssalts = 0.11–0.25%) to extreme (Ssalts = 1.35–2.57%). The main contributors to salinity were Na+, Ca2+, and Mg2+. Multivariate analysis revealed that biomass accumulation is positively affected by moderate/high salinity in 20–60 cm soil layers, which may be associated with the salt required for the optimal growth of the halophyte B. prostrata. The formation of seed genetic diversity is negatively affected by slight/moderate salinity in the 0–40 cm layers. An increase in divalent ion content can reduce genetic diversity and increase the local adaptation of B. prostrata to magnesium–calcium sulfate salinity. The effect of the in-depth distribution of soil salinity on productivity and genetic diversity may be related to seasonal variables during biomass accumulation (summer) and seed formation (autumn).

1. Introduction

Salinity is a significant environmental problem that limits plant productivity, especially in arid and semiarid regions that cover approximately 40% of the globe. Semiarid regions are projected to become drier and more saline due to rising global temperatures [1,2,3]. Vegetation survival and productivity are primarily regulated by the water balance in soil, which affects the water balance and photosynthetic rate in plants [4]. Soils in drylands are usually heterogeneous in space and time due to the presence of biotic and abiotic elements. Spatiotemporal variations in soil salinity and water content are well documented [5]. Salinity amplifies the effects of soil drought on plants by creating additional osmotic pressure. Soil is considered saline when the salt content exceeds 3–5 g salt/L in the soil solution, when electrical conductivity (EC) exceeds 2–4 dS/m, or when the sum of salts exceeds 0.15–0.2%, creating osmotic pressure above 0.2 MPa, which significantly reduces the yield of the most crops [2,6]. Salinity reduces plant growth and prematurely ages mature leaves, which leads to a decrease in the functional leaf area. A decrease in plant biomass is also influenced by Na+ and Cl toxicity and the accompanying oxidative stress [2,7]. Halophytes are highly salt-tolerant plants but underutilized resources that occupy naturally saline soil environments in coastal estuaries and inland salt flats in arid and semiarid zones [8]. Nowadays, climate-smart agriculture (CSA) practices increasingly use wild salt-tolerant species (halophytes) to restore the grazing capacity of degraded pastures, provide forage for livestock and utilize oilseeds and medicinal and aromatic plants [7,9]. Apart from these applications, halophytes play a significant role in the maintenance of ecosystem functions and sustainability [10,11].
Genetic diversity provides plants with the ability to adapt and survive in changing environments, including soil chemistry variability. The genetic architecture of a population plays a fundamental role in the origin and maintenance of local adaptation [12]. The degree of local adaptation is largely determined by the interaction between selection and gene flow along ecological gradients. Different types of selection can operate under natural conditions: (i) conditionally neutral selection occurs when two alleles do not have an advantage in fitness in one environment but differ in fitness in another environment; (ii) environmentally antagonistic selection, when different alleles are locally adapted to different environments, conferring higher fitness there [12]. ‘Fitness’ is often viewed as the ability to withstand adverse conditions; however, from an evolutionary perspective, fitness is defined as the ability of an individual to spread their genes through offspring. Thus, in plants, fitness depends on the number of seeds that a plant can successfully produce under adverse environmental conditions [13]. A common plant response to environmental stresses is a decrease in fertility, which consists of aborting ovules and/or pollen and redirecting resources from reproductive activity into metabolic reactions for stress tolerance [14]. Plants are reported to control the consumption of maternal resources at several stages of development by regulating the number of flowers, gametophytes, and embryos that develop further [14]. This type of developmental regulation can lead to the favored selection of certain alleles or genotypes, producing genotype–environment associations and/or interactions [15]. The survival rate and adaptation of populations in different and changing environments depend on the genetic diversity of the seed pool. For example, diversity in the genetic composition of seeds allows Atriplex tatarica to survive under distinct conditions: heterozygous plants mainly germinate under optimal conditions, and homozygous plants typically germinate under suboptimal conditions [16]. Ecological factors that influence reproduction and seed dispersal are, therefore, particularly important aspects in shaping genetic diversity and population structures. Edaphic conditions, such as soil type, pH, nutrients, moisture, and the depth of soil layers, can significantly affect the level of genetic diversity and local adaptation in plant populations [15,17,18,19]. For example, Phragmites australis populations with high genetic diversity have a high tolerance to soil salinity [20]. The high genetic diversity of populations is fundamental to the long-term survival success of a plant species [21].
In unfavorable environments, such as areas of high soil salinity, plants are forced to seek a ‘compromise’ between productivity and adaptation, which depends on genetic diversity at the population level. Within the soil profile, salinity is a dynamic indicator; it changes with depth and according to seasons [5]. In turn, plants have a vertical fine-root distribution, which determines the possibility of acquiring resources along the soil profile, since plants rely mainly on their fine roots to acquire belowground water and nutrient resources [22]. To assess and predict the productivity and adaptation of species under changing conditions, it is necessary to understand how the salinization of different soil layers affects biomass and genetic polymorphism formation. A convenient model species for these purposes is the polymorphic widespread halophyte Bassia prostrata (L.) A.J. Scott (Kochia prostrata (L.) Schrad.) (subfamily Chenopodiaceae), with a significant variety of morphological, biochemical, and ecological–physiological properties; high genetic polymorphism; and wide ecological plasticity [23,24,25,26,27]. Moreover, the effect of soil conditions on both the level of diversity and genetic structure of B. prostrata populations has been shown [28].
The present study aims to investigate the effects of the level and chemistry of salinity within different soil layers (including horizontal and vertical variations in the soil characteristics) on the productivity and genetic diversity of the halophyte B. prostrata to clarify the adaptive mechanism it uses to withstand fluctuations of salt accumulation along soil depth profiles.

2. Materials and Methods

2.1. Study Area

Studies were carried out in the northwestern Caspian Lowland (Russia) (Figure 1A). The region is a flat marine accumulative plain, which is characterized by the almost complete absence of surface and subsurface runoff. According to climate parameters (Figure 1B), the region is arid, with an average annual temperature of 8.7 °C and precipitation of 291 mm. In Caspian Lowland plain landscapes, solonetzic complexes are widespread; depressions and other negative relief elements (microdepressions, depressions, estuaries) are characterized by dark-colored chernozem-like or meadow–chestnut soils [29]. Seven typical habitats of B. prostrata were selected for the study based on differences in the soil salinity levels (No. 1–7 in Figure 1A). Five of them were located near the salt lakes Bulukhta and Elton at different distances from the coastline. The other two habitats were in the plain part between these lakes (Figure 1A). B. prostrata habitats were characterized mainly by solonetzic and/or light chestnut solonetzic soils and desert steppe vegetation.

2.2. Plant Sampling

Bassia prostrata (L.) A.J. Scott (Kochia prostrata (L.) Schrad.) (Chenopodiaceae) is a typical perennial C4 halophyte native to arid and semiarid rangelands in Central Eurasia and the Western United States. B. prostrata naturally occurs in all kinds of soils, such as saline, sandy, rocky, and poor soils [24,30,31]. B. prostrata has a thick, woody root system that can penetrate 3–6.5 m depths and lateral roots stretching 130–160 cm that mine for moisture in the upper (up to 60 cm) soil layers [30,31]. This is the reason for studying the upper soil layers: approximately 0–20 cm, 20–40 cm, and 40–60 cm. Soils, plants, and seeds were sampled in seven typical habitats of B. prostrata (No. 1–7 in Figure 1A). The aboveground parts of five plants were harvested in each habitat in the middle of September for biomass analysis. More than 100 seeds from 10 to 15 mother plants from each habitat (population) were collected at the beginning of November and combined to generate a seed pool for population genetic analysis.

2.3. Soil Sampling and Analysis

Seven habitats of B. prostrata soil pits (Nos. 14, 11, 15, 10, 18, 7, and 6, corresponding to habitats Nos. 1 to 7 in Figure 1A) were excavated. Profiles were examined to depths of 0 to 60 cm. Three soil samples (n = 3) were used for the analysis of each soil layer of each habitat. Chemical and physicochemical analyses were performed at the Analytical Laboratory of the V.V. Dokuchaev Soil Science Institute using standard methods [32]. Calcium and magnesium concentrations in water extracts (1:5) were determined with the complexometric titration method; sodium and potassium concentrations were determined with the flame photometry method; the total alkalinity was determined using titration with sulfuric acid (with methyl orange indicator); the concentration of chlorine ions was determined with argentometry (according to Mohr); and the concentration of sulfate ions was determined using titration with BaCl2. The content of ions Na+, K+, Ca2+, Mg2+, Cl, SO42−, and HCO3 are presented in cmol(eq)/kg. The sum of salts (Ssalts) represents the sum of the mass fraction of ions from the solid soil residue (%) [6].

2.4. Plant Biomass and Water Content

Plant height, fresh (FW) and dry (DW) biomass, and water content (W) were assessed for aboveground parts of B. prostrata plants (n = 5) from seven habitats. Biomass was estimated for fresh and dry shoots. Plant samples were dried at 80 °C for two days until reaching a constant mass to quantitatively measure the dry shoot matter. The water content in the shoots was calculated according to the following formula:
W = (FW − DW)/DW.

2.5. Population Genetic Analysis

Genetic diversity can be studied using neutral markers (based on differences in DNA sequences) and partially selective markers (isozymes), which can reflect changes in environmental conditions [33,34]. In this study, we used isozymes (alternative forms of the enzymes encoded by different alleles of the same gene) to assess the genetic diversity of the populations.
For each population of B. prostrata, 50 seeds from the seed pool (more than 100 seeds from 10 to 15 mother plants) were germinated, and all good germinated seeds (n = 25–35 per population) were analyzed for genetic polymorphism. Population genetic analysis was performed on embryos using starch gel electrophoresis of the following enzymatic systems: glutamate oxaloacetate transaminase (GOT (AAT), E.C. 2.6.1.1), diaphorase (DIA, E.C. 1.6.99), glutamate dehydrogenase (GDH, E.C. 1.4.1.2), superoxide dismutase (SOD, E.C. 1.15.1.1), glucose-6-phosphate dehydrogenase (G6PD, E.C. 1.1.1.49), 6-phosphogluconate dehydrogenase (6PGD, E.C. 1.1.1.44), malate dehydrogenase (MDH, E.C. 1.1.1.37), and malic enzyme (Me, E.C. 1.1.1.40). The seeds were cleaned of their wings and soaked in water for 12 h and homogenized in 80 μL of Tris-HCl buffer with KCl, MgCl2, EDTA, Triton X-100, and PVP. Enzymes were separated in 10% starch gel using two buffer systems. In system 1, the electrode buffer was 160 mM Tris–50 mM citric acid, pH 8.0; the gel buffer was prepared by diluting 10 mL of the electrode buffer with 90 mL H2O. In system 2, the electrode buffer was 300 mM boric acid–60 mM NaOH, pH 8.2; the gel buffer was 80 mM Tris–9 mM citric acid, pH 8.7. Electrophoresis was performed at 90 V, 40–50 mA in buffer system 1 or at 210 V, and 70–80 mA in buffer system 2 for 4–6 h at 5 °C. Staining of particular enzymes and genetic interpretation of the results followed standard techniques according to Soltis and Soltis [35] and Spooner et al. [33]. The level of genetic polymorphism was estimated by calculating observed (Ho) and expected (He) heterozygosity for each polymorphic loci and by calculating the proportion of polymorphic loci (P99) and the average (for all loci) observed (Ho) and expected (He) heterozygosity in POPGEN 1.32.

2.6. Statistical Analysis

Principal component analysis (PCA) was carried out using R software (version 3.6.1). Table 1 and Figure 2 show the means of the obtained values and their standard errors (n = 3 for soil samples and n = 5 for plant samples).

3. Results

3.1. Soil Characteristics

The soils in B. prostrata habitats (populations) differed significantly in the degree and vertical changes in salinity chemistry. In each of the seven habitats, the soil salinity the 0–20 cm, 20–40 cm, and 40–60 cm in layers was studied. Two habitats (Nos. 1 and 2) had non-saline or slightly saline soils (Table 1). The soils in the other five habitats (Nos. 3–7) were much more saline: the upper 0–20 cm layers were non-saline or slightly saline (Ssalts = 0.11–0.25%); the 20–40 cm layers were moderately or highly saline (Ssalts = 0.5–1.17%); and the 40–60 cm layers were highly or extremely saline (Ssalts = 1.35–2.57%) (Table 1). In all habitats, except for No. 4, Na+ was the dominant cation: 0.03–2.27 cmol(eq)/kg, 0–20 cm layer; 0.32–8.95 cmol(eq)/kg, 20–40 cm layer; and 3.15–23.25 cmol(eq)/kg, 40–60 cm layer. The Ca2+ ion predominated in habitat No. 4. In other soils, Ca2+ and Mg2+ contents also significantly contributed to salinity at 0.28–12.9 and 0.26–8.32 cmol(eq)/kg, respectively. Chlorides and sulfates were the dominant anions (Table 1).
Table 1. Contents of anions and cations in soils of the seven Bassia prostrata habitats in the northwestern Caspian Lowland.
Table 1. Contents of anions and cations in soils of the seven Bassia prostrata habitats in the northwestern Caspian Lowland.
Habitats,
No
Soil LayersAnions, cmol(eq)/kgCations, cmol(eq)/kgSsalt, %Salinity Level
HCO3−ClSO42−Ca2+Mg2+Na+K+
10–201.04 ± 0.080.26 ± 0.020.78 ± 0.040.78 ± 0.020.52 ± 0.040.56 ± 0.010.22 ± 0.040.15non-saline
20–401.25 ± 0.110.17 ± 0.010.52 ± 0.030.52 ± 0.010.26 ± 0.031.45 ± 0.120.02 ± 0.010.16non-saline
40–602.18 ± 0.170.26 ± 0.030.52 ± 0.030.52 ± 0.020.78 ± 0.081.74 ± 0190.03 ± 0.010.23slight
20–200.10 ± 0.020.17 ± 0.010.52 ± 0.030.26 ± 0.010.26 ± 0.010.24 ± 0.010.03 ± 0.010.05non-saline
20–400.31 ± 0.020.34 ± 0.040.26 ± 0.010.26 ± 0.020.26 ± 0.010.32 ± 0.040.07 ± 0.010.06non-saline
40–600.94 ± 0.131.89 ± 0.141.82 ± 0.230.78 ± 0.660.78 ± 0.323.15 ± 0.070.04 ± 0.010.31slight
30–201.35 ± 0.080.34 ± 0.070.52 ± 0.010.52 ± 0.020.26 ± 0.011.31 ± 0.040.12 ± 0.010.17slight
20–401.77 ± 0.215.41 ± 0.431.04 ± 0.080.52 ± 0.010.78 ± 0.037.01 ± 0.160.01 ± 0.010.50moderate
40–600.52 ± 0.0210.22 ± 0.9318.98 ± 0.219.88 ± 0.108.32 ± 0.7211.48 ± 0.810.04 ± 0.011.87extreme
40–200.94 ± 0.100.26 ± 0.031.56 ± 0.332.08 ± 0.500.26 ± 0.090.14 ± 0.070.27 ± 0.050.20slight
20–400.57 ± 0.040.17 ± 0.0115.60 ± 0.4212.09 ± 0.743.12 ± 0.411.84 ± 0.090.15 ± 0.011.11moderate
40–600.52 ± 0.030.69 ± 0.0818.72 ± 1.539.88 ± 0.513.64 ± 0.336.27 ± 0.710.14 ± 0.021.35high
50–200.73 ± 0.170.49 ± 0.0.10.35 ± 0.070.43 ± 0.080.43 ± 0.110.74 ± 0.260.06 ± 0.020.11non-saline
20–401.25 ± 0.767.90 ± 0.062.60 ± 0.321.04 ± 0.090.52 ± 0.0710.28 ± 0.930.01 ± 0.010.75moderate
40–600.31 ± 0.027.39 ± 0.0723.92 ± 1.3010.14 ± 0.126.76 ± 0.5414.77 ± 1.100.05 ± 0.012.06extreme
60–200.78 ± 0.130.39 ± 0.040.91 ± 0.110.52 ± 0.010.52 ± 0.010.78 ± 0.070.26 ± 0.090.15non-saline
20–401.77 ± 0.173.18 ± 1.054.03 ± 0.981.04 ± 0.210.39 ± 0.117.50 ± 1.940.04 ± 0.010.76moderate
40–600.42 ± 0.037.65 ± 1.1122.36 ± 1.7411.70 ± 1.038.06 ± 0.9210.67 ± 1.050.10 ± 0.031.95extreme
70–201.51 ± 0.041.42 ± 0.741.69 ± 0.320.52 ± 0.010.39 ± 0.113.51 ± 1.010.20 ± 0.110.25slight
20–400.73 ± 0.049.02 ± 1.088.58 ± 0.913.12 ± 0.072.08 ± 0.3413.14 ± 1.110.09 ± 0.011.17high
40–600.62 ± 0.0316.06 ± 1.7723.66 ± 1.7310.66 ± 0.856.50 ± 0.7723.25 ± 2.760.04 ± 0.012.57extreme
Ssalt—the sum of salts represents the sum of the mass fraction of ions from the solid soil residue (%). Values are means ± standard errors (n = 3).

3.2. Plant Growth and Water Content

B. prostrata plants varied significantly in growth parameters between populations (Figure 2). The greatest plant heights were in populations Nos. 2, 4, and 7 (Figure 2A), while the highest fresh (FW) and dry (DW) biomass aboveground parts of plants were observed in populations No. 4 and No. 6 (Figure 2B,C). At the same time, the water content (W) in the plants was the highest in population No. 7 (Figure 2D).
Figure 2. Growth parameters and water content of Bassia prostrata plants from seven populations. 1–7—populations; (A)—plant height; (B)—fresh biomass (FW) aboveground part of plants; (C)—dry biomass (DW) aboveground part of plants; (D)—water content (W) of plants. Values are means ± standard errors (n = 5).
Figure 2. Growth parameters and water content of Bassia prostrata plants from seven populations. 1–7—populations; (A)—plant height; (B)—fresh biomass (FW) aboveground part of plants; (C)—dry biomass (DW) aboveground part of plants; (D)—water content (W) of plants. Values are means ± standard errors (n = 5).
Life 13 00056 g002

3.3. Population Genetic Diversity

An analysis of eight enzyme systems in seven B. prostrata populations revealed ten loci; one of them (Sod) was monomorphic in all populations. The other nine loci were polymorphic: G6pd in all populations; Me in six populations; Gdh in four populations; Got, 6pgd, and Mdh-A in 3 populations; and Dia-A, Dia-B, and Mdh-B in one population. Values of observed heterozygosity (Ho) varied from 5 to 47% among polymorphic loci and populations (Figure 3A), whereas expected heterozygosity (He) varied from 5% to 59% (Figure 3B). The average (for all loci) observed heterozygosity varied from 5.5% to 11.1%, and expected heterozygosity varied from 6.2% to 15.9% in populations of B. prostrata (Figure 3C). The polymorphic loci proportion (P) among the populations was 20–70% (Figure 3C). On average, populations Nos. 2, 3, 5, and 6 were more polymorphic than populations Nos. 1, 4, and 7 (Figure 3).

3.4. Plant–Soil Interaction

Principal component analysis (PCA) did not reveal significant correlations between B. prostrata fresh and dry biomass and soil properties in 0–20 cm soil layers (Figure 4A). There were significant positive correlations between B. prostrata fresh and dry biomass and the sum of salts and the sum of the contents of anions Ca2+, Mg2+, and SO42− in the 20–40 cm and, to a lesser degree, 40–60 cm soil layers (Figure 4B), as well as with K+ content in 40–60 cm layers (Figure 4C).
PCA revealed the negative dependencies of genetic polymorphism parameters (P, He, and Ho) on K+, Ca2+, and sulfate ions contents and, to a lesser degree, on the sum of salts and the sum of anions in the 0–20 cm soil layers (Figure 4D). In addition, a negative correlation was observed between P, He, and Ho from one side and Mg2+, K+, Ca2+, and SO42− contents in 20–40 cm soil layers from the other side (Figure 4E). There were no correlations between genetic polymorphism parameters and soil properties in the 40–60 cm layers (Figure 4F).

4. Discussion

The habitats of Bassia prostrata in this study were characterized by significant diversity in the degree and chemistry of soil salinity; high salinity occurred at different soil depths (Table 1). B. prostrata has wide edaphic plasticity and can grow on various soil genesis, e.g., chestnut, light-chestnut alkaline soils, and solonetz, as well as on soil-forming rocks of different compositions, from light sandy to heavy loamy, stony, and gypsum [30,36].
Our results revealed differences in correlations between B. prostrata aboveground biomass accumulation and seed genetic polymorphism and the chemistry and degree of salinity of different soil layers. The genetic diversity level was affected by the salinity degree and the chemistry of the uppermost soil layers (0–20 cm, 20–40 cm), and biomass accumulation was mainly affected by the salinity of the 20–40 cm and 40–60 cm soil layers. Such differences may be associated with different seasons of aboveground biomass and seed pool formation. B. prostrata biomass accumulation (before flowering) occurs mainly in the summer, the hottest and driest season: 23–26 °C, 40–43% humidity, and 65.7 mm precipitation (Figure 1B). In the summer, the drying of the uppermost soil layers can be observed, and plants receive water and dissolved salt ions from lower soil layers, affecting biomass formation. Our study showed a positive dependence of B. prostrata productivity on the degree of salinity in 20–40 cm soil layers (Figure 4B). B. prostrata, as a halophyte, requires a certain amount of salt in the substrate for optimal growth [37] and has high productivity in soils with 20 dS/m (EC) salinity [31]. The content of the main plant nutrient K+ in seven soil habitats decreased from the upper to lower layers, whereas the Na+ concentration increased (means of K+/Na+ were 0.45 and 0.01 in the 0–20 cm and 40–60 cm soil layers, respectively; Table 1). Despite the fact that plants growing in saline habitats have acquired mechanisms that allow for selective uptake of K+ when Na+ dominates in the substrate [37], in B. prostrata plants, K+ content in tissues decreased when Na+ exceeded 100–200 mM NaCl [38]. Thus, the selective absorption of K+ from the 40–60 cm soil layer under conditions of increased competition with Na+ affects B. prostrata biomass accumulation in natural habitats (Figure 4C).
Ca2+ and Mg2+ ions are also essential mineral nutrients. Ca2+ is a universal signal in all eukaryotic cells and participates in many other cellular processes, for example, in the maintenance of cell membrane integrity, cation–anion balance, and osmoregulation [39,40]. Mg2+ is an activator of more than 300 enzymes, in particular, photosynthetic and respiratory ones, which are also needed for DNA and RNA synthesis [41,42]. It is well known that Ca2+ plays a protective role in a plant’s response to salinity. Much less is known about the role of Mg2+ in the salt tolerance of plants [39]. However, it was shown that low concentrations of mixed salts with CaCl2, and MgSO4 are necessary for the successful seed germination of B. prostrata [43]. Our study showed that Ca2+ and Mg2+ contents contributed significantly to soil salinity in B.prostrata habitats (Table 1). Positive correlations between biomass accumulation and Ca2+ and Mg2+ contents in the 20–40 cm soil layer (Figure 4B) indicate their necessity for B. prostrata growth. The influence of magnesium in this soil layer can be associated with the optimal K+/Mg2+ ratio. The K+/Mg2+ ratio for soils and plant tissues is critical to maintaining optimal plant nutrition and, hence, plant productivity [42]. The K+/Mg2+ ratio (0.09 ± 0.03) in the 20–40 cm soil layer in B. prostrata habitats was less than that of the 0–20 cm soil layer (0.56 ± 0.17) but higher than that of the 40–60 cm soil layer (0.02 ± 0.01).
B. prostrata seeds are formed in autumn, during a cooler and rainier period (1–16 °C, 49–81% humidity, 77.1 mm precipitation; Figure 1B) when the upper soil layers are moist and plants receive water and dissolved salt ions from them. At the same time, the need for water decreases due to lower air temperatures and higher humidity. Therefore, the formation of seed genetic diversity in B. prostrata, upon which the future stability of populations in changing environments depends [12,44,45], is affected by the salinity level and ionic composition of the 0–40 cm soil layers. In heterogeneous environments, the processes of gene flow, mutation, and sexual reproduction generate local genetic variation, providing material for local adaptation [45]. The influence of soil factors such as soil type, pH, moisture, and soil layer depth on population genetic diversity has been demonstrated in different plant species [15,17,18,19]. A nine-year experiment on the influence of soil moisture and nitrogen, phosphorus, and potassium content in soil on allozyme frequency revealed an allele–habitat association in Festuca ovina [15]. It was found that in natural populations the Pgi-2-2 allele is significantly associated with soil moisture and is affected by nutrient/water treatments [15]. Negative correlations between B. prostrata genetic diversity with inorganic ion content (except for Na+ and Cl) and the sum of salts in the 0–40 cm soil layers (Figure 4D,E) indicate selection in favor of homozygotes. Since isozymes (allozymes) were also used in our study, a question arises regarding the functional significance of enzymes under selection. Loci G6pd and Me were the most polymorphic among the B. prostrata populations (Figure 3A). They encode the enzymes glucose-6-phosphate dehydrogenase (G6PD) and malik-enzyme (NADP-Me), respectively, which are associated with the regulatory nodes of dark respiration and photosynthesis. G6PD is a key enzyme in the alternative apotomous oxidative pentose phosphate pathway (OPPP), whose role is enhanced under stress [46]. Malik-enzyme is involved in photosynthesis and is especially active in C4 species, and it plays a vital role in the tolerance to salt stress [47]. The adaptive–compensatory reactions of plants under stress are always associated with additional energy costs, which leads to a change in the balance between photosynthesis and respiration [46]. Any shifts in this balance are reflected in the total plant productivity. Selection leads to local adaptation, and the strength of local adaptation depends on the strength of selection. Strong selection leads to strong local adaptation, which is significantly affected by landscape heterogeneity [48]. The negative influence of Ca2+, Mg2+, and SO42− contents in the 0–40 cm soil layer on heterozygosity indicates the formation of the local adaptation of B. prostrata to magnesium–calcium sulfate soil salinity. The detected level of sodium chloride salinity did not negatively impact seed genetic polymorphism (Figure 4D,E). This is probably due to the necessity of these ions in maintaining water balance in the aboveground organs of B. prostrata (Figure 4C).

5. Conclusions

Our study demonstrates that in natural habitats the productivity and seed genetic polymorphism of halophytes may be affected by the salinity of different soil layers. These differential plant responses to vertically heterogeneous soil salinity could be attributed to seasonal variables during biomass accumulation (summer) and seed formation (autumn). An excess of some ions in the uppermost soil layers can lead to increased local adaptation to a certain type of salinity and the appearance of genotype-environment associations. Genotype–environment association analyses may allow us to develop adaptive measures for natural resource management, pasture improvement, and the phytoremediation and restoration of lands with different salinity chemistries.

Author Contributions

Conceptualization, E.S., M.L., and K.T.; methodology, E.S. and M.L.; software, M.P.; validation, E.S., K.T. and M.L.; formal analysis, A.K.; investigation, E.S., A.K. and M.L.; resources, E.S. and K.T.; data curation, A.K.; writing—original draft preparation, E.S.; writing—review and editing, E.S., K.T. and M.L.; visualization, M.P.; supervision, K.T.; project administration, E.S. All authors have read and agreed to the published version of the manuscript.

Funding

This research was supported by the Science and Technology Research Partnership for Sustainable Development (SATREPS), in collaboration with the Japan Science and Technology Agency (JST, JPMJSA2001) and the Japan International Cooperation Agency (JICA), and by the Ministry of Science and Higher Education of the Russian Federation (theme No. 122042700044-6) and FGUR-2022-001- 1021060307664-8-4.1.4.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

The data are available from the authors upon request.

Acknowledgments

We are grateful to the administration of the Elton Regional Nature Park for the opportunity to conduct research in the park.

Conflicts of Interest

The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.

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Figure 1. The location of seven populations of Bassia prostrata (A) and the long-term (2007–2018) average atmospheric temperature and precipitation (B) of the northwestern Caspian Lowland. 1–7—numbers of populations (habitats); Tm—temperature; P—precipitation.
Figure 1. The location of seven populations of Bassia prostrata (A) and the long-term (2007–2018) average atmospheric temperature and precipitation (B) of the northwestern Caspian Lowland. 1–7—numbers of populations (habitats); Tm—temperature; P—precipitation.
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Figure 3. Genetic polymorphism in seven populations of Bassia prostrata. (A)—observed heterozygosity (Ho) of polymorphic loci; (B)—expected heterozygosity (He) of polymorphic loci; (C)—polymorphic loci proportion of population (P), average (for all loci) observed heterozygosity (Ho), and average (for all loci) heterozygosity (He) of seven populations of Bassia prostrata.
Figure 3. Genetic polymorphism in seven populations of Bassia prostrata. (A)—observed heterozygosity (Ho) of polymorphic loci; (B)—expected heterozygosity (He) of polymorphic loci; (C)—polymorphic loci proportion of population (P), average (for all loci) observed heterozygosity (Ho), and average (for all loci) heterozygosity (He) of seven populations of Bassia prostrata.
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Figure 4. Principle component analysis (PCA) of growth (AC), genetic diversity (DF) parameters of Bassia prostrata, and salinity of 0–20 cm (A,D), 20–40 cm (B,E), and 40–60 cm (C,F) soil layers. K+, Na+, Ca2+, Mg2+, Cl, SO42−, HCO3—ions content in soil; Ss—sum of salts; Sa—sum of anions in soil; FW—fresh plant biomass; DW—dry plant biomass; W—water content in leaves; P—proportion of polymorphic loci; Ho—observed heterozygosity; and He—expected heterozygosity of B. prostrata.
Figure 4. Principle component analysis (PCA) of growth (AC), genetic diversity (DF) parameters of Bassia prostrata, and salinity of 0–20 cm (A,D), 20–40 cm (B,E), and 40–60 cm (C,F) soil layers. K+, Na+, Ca2+, Mg2+, Cl, SO42−, HCO3—ions content in soil; Ss—sum of salts; Sa—sum of anions in soil; FW—fresh plant biomass; DW—dry plant biomass; W—water content in leaves; P—proportion of polymorphic loci; Ho—observed heterozygosity; and He—expected heterozygosity of B. prostrata.
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Shuyskaya, E.; Toderich, K.; Kolesnikov, A.; Prokofieva, M.; Lebedeva, M. Effects of Vertically Heterogeneous Soil Salinity on Genetic Polymorphism and Productivity of the Widespread Halophyte Bassia prostrata. Life 2023, 13, 56. https://doi.org/10.3390/life13010056

AMA Style

Shuyskaya E, Toderich K, Kolesnikov A, Prokofieva M, Lebedeva M. Effects of Vertically Heterogeneous Soil Salinity on Genetic Polymorphism and Productivity of the Widespread Halophyte Bassia prostrata. Life. 2023; 13(1):56. https://doi.org/10.3390/life13010056

Chicago/Turabian Style

Shuyskaya, Elena, Kristina Toderich, Alexander Kolesnikov, Maria Prokofieva, and Marina Lebedeva. 2023. "Effects of Vertically Heterogeneous Soil Salinity on Genetic Polymorphism and Productivity of the Widespread Halophyte Bassia prostrata" Life 13, no. 1: 56. https://doi.org/10.3390/life13010056

APA Style

Shuyskaya, E., Toderich, K., Kolesnikov, A., Prokofieva, M., & Lebedeva, M. (2023). Effects of Vertically Heterogeneous Soil Salinity on Genetic Polymorphism and Productivity of the Widespread Halophyte Bassia prostrata. Life, 13(1), 56. https://doi.org/10.3390/life13010056

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