Induction of Triticale (×Triticosecale Wittmack) In Vitro Androgenesis in Anther Cultures of F1 Hybrid Combinations, Varieties and Homogeneity Testing of Offspring Generation
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials and Growing Conditions of Donor Plants
2.2. Pre-Treatment of Donor Tillers
2.3. Isolation of Anthers and In Vitro AC
2.4. In Vitro Plant Regeneration and Acclimatization of Plantlets
2.5. Primers
2.6. DNA Isolation, PCR, Microsatellite Analysis, Allele Size Determination
2.7. Statistical Analysis
3. Results
3.1. Androgenesis Induction in Triticale (×Triticosecale Wittmack)
3.2. Testing of Homogeneity with Molecular Markers
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Source of Variance | df | MS—ELS | MS—Green Plantlets | MS—Albinos | MS—Transplanted Plantlets |
---|---|---|---|---|---|
Induction medium | 1 | 4145.636 * | 159.1225 ns | 72.7273 * | 210.182 * |
Genotype | 8 | 35,588.25 *** | 2926.648 *** | 42.2263 ** | 1019.25 *** |
Interaction | 8 | 721.4058 ns | 94.3030 ns | 23.0884 ns | 103.5818 ** |
Error | 54 | 910.6944 | 99.4634 | 12.5278 | 39.2424 |
Source of Variance | df | MS—Plant Regeneration Efficiency | MS—Green Plant Regeneration Efficiency |
---|---|---|---|
Genotype | 8 | 234.7103 *** | 285.0326 *** |
Induction medium | 1 | 3.2918 ns | 23.9410 ns |
Error | 8 | 26.5776 | 19.6739 |
Genotype | Number of Transplanted Plantlets | Haploids | Spontaneous DH | Percentage of Spontaneous Chromosome Doubling * (%) |
---|---|---|---|---|
‘Hungaro’ | 175 | 131 | 44 | 25.1 |
‘H × Sz’ | 26 | 14 | 12 | 46.2 |
‘H × D’ | 43 | 14 | 29 | 67.4 |
‘GK Szemes’ | 3 | 2 | 1 | 33.3 |
‘Sz × H’ | 82 | 64 | 18 | 22.0 |
‘Sz × D’ | 36 | 29 | 7 | 19.4 |
‘Dimenzio’ | 7 | 5 | 2 | 28.6 |
‘D × H’ | 87 | 62 | 25 | 28.7 |
‘D × Sz’ | 55 | 42 | 13 | 23.6 |
‘H × R’ | 351 | 256 | 95 | 27.1 |
‘D × R’ | 187 | 125 | 62 | 33.2 |
Total | 1052 | 744 | 308 | 29.3 |
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Kruppa, J.; Kanbar, O.Z.; Tóth-Lencsés, K.A.; Kiss, E.; Bóna, L.; Lantos, C.; Pauk, J. Induction of Triticale (×Triticosecale Wittmack) In Vitro Androgenesis in Anther Cultures of F1 Hybrid Combinations, Varieties and Homogeneity Testing of Offspring Generation. Life 2023, 13, 1970. https://doi.org/10.3390/life13101970
Kruppa J, Kanbar OZ, Tóth-Lencsés KA, Kiss E, Bóna L, Lantos C, Pauk J. Induction of Triticale (×Triticosecale Wittmack) In Vitro Androgenesis in Anther Cultures of F1 Hybrid Combinations, Varieties and Homogeneity Testing of Offspring Generation. Life. 2023; 13(10):1970. https://doi.org/10.3390/life13101970
Chicago/Turabian StyleKruppa, József, Osama Zuhair Kanbar, Kitti Andrea Tóth-Lencsés, Erzsébet Kiss, Lajos Bóna, Csaba Lantos, and János Pauk. 2023. "Induction of Triticale (×Triticosecale Wittmack) In Vitro Androgenesis in Anther Cultures of F1 Hybrid Combinations, Varieties and Homogeneity Testing of Offspring Generation" Life 13, no. 10: 1970. https://doi.org/10.3390/life13101970