Natural Co-Exposure to Borrelia burgdorferi s.l. and Anaplasma phagocytophilum: Unraveling the Hematological Profile in Sheep
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animal Selection Criteria
2.2. Blood Sampling
2.3. Complete Blood Count
2.4. Indirect Immunofluorescence Antibody (IFA) Assay
2.5. Groups
2.6. Data Analysis
3. Results
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Groups | B. burgdorferi | A. phagocytophilum | |
---|---|---|---|
IgM | IgG | IgG | |
BorIgM+ | + | − | − |
BorIgG+ | − | + | − |
BorIgG&M+ | + | + | − |
An+BorIgG+ | − | + | + |
An+BorIgG&M+ | + | + | + |
An+ | − | − | + |
Control | − | − | − |
Seropositivity | PCV | PLTs |
---|---|---|
BorIgM+ | −5.03 (0.81) *** | −19,157 (34,669) NS |
BorIgG+ | −4.97 (0.66) *** | −142,795 (28,295) *** |
BorIgG&M+ | −8.53 (0.95) *** | −356,990 (40,750) *** |
An+BorIgG+ | −4.81 (0.81) *** | −184,045 (34,669) *** |
An+BorIgG&M+ | −9.23 (0.83) *** | −408,285 (35,711) *** |
An+ | −2.10 (1.00) * | −22,733 (42,808) NS |
Seropositivity | WBC | Neutrophils | Lymphocytes |
---|---|---|---|
BorIgM+ | −1184 (517.835) * | −773 (294) ** | −232 (334) NS |
BorIgG+ | 685 (422) NS | 712 (240) ** | 10 (273) NS |
BorIgG&M+ | −3804 (608) *** | −1309 (346) *** | −2412 (393) *** |
An+BorIgG+ | −2040 (517) *** | −724 (294) * | −923 (334) ** |
An+BorIgG&M+ | −6466 (533) *** | −3165 (303) *** | −2937 (344) *** |
An+ | −2513 (639) *** | −2058 (363) *** | −536 (413) NS |
Seropositivity | Monocytes | Eosinophils |
---|---|---|
BorIgM+ | −77 (29) * | −100 (27) *** |
BorIgG+ | −10 (23) NS | −27 (22) NS |
BorIgG&M+ | 10 (34) NS | −127 (32) *** |
An+BorIgG+ | −108 (29) *** | −282 (27)*** |
An+BorIgG&M+ | −122 (30) *** | −241 (28) *** |
An+ | 46 (36) NS | 36 (34) NS |
Seropositivity | Anemia | Thrombocytopenia | Leukopenia | Neutropenia | Lymphocytopenia |
---|---|---|---|---|---|
BorIgM+ | 12.21 *** | 10.38 *** | 1.08 NS | 0.00 NS | 0.00 NS |
BorIgG+ | 16.14 *** | 20.75 *** | 0.60 NS | 3.86 NS | 0.00 NS |
BorIgG&M+ | 103.75 *** | 0.00 NS | 35.00 *** | 42.50 *** | 28.00 *** |
An+BorIgG+ | 30.18 *** | 41.50 *** | 3.50 NS | 0.00 NS | 0.00 NS |
An+BorIgG&M+ | 498.00 *** | 0.00 NS | 0.00 NS | 0.00 NS | 30.33 *** |
An+ | 6.92 * | 1.38 NS | 21.78 *** | 19.32 *** | 4.00 NS |
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Athanasiou, L.V.; Tsokana, C.N.; Gougoulis, D.A.; Tzivara, A.H.; Dedousi, A.; Katsoulos, P.D. Natural Co-Exposure to Borrelia burgdorferi s.l. and Anaplasma phagocytophilum: Unraveling the Hematological Profile in Sheep. Life 2023, 13, 469. https://doi.org/10.3390/life13020469
Athanasiou LV, Tsokana CN, Gougoulis DA, Tzivara AH, Dedousi A, Katsoulos PD. Natural Co-Exposure to Borrelia burgdorferi s.l. and Anaplasma phagocytophilum: Unraveling the Hematological Profile in Sheep. Life. 2023; 13(2):469. https://doi.org/10.3390/life13020469
Chicago/Turabian StyleAthanasiou, Labrini V., Constantina N. Tsokana, Dimitris A. Gougoulis, Athanasia H. Tzivara, Anna Dedousi, and Panagiotis D. Katsoulos. 2023. "Natural Co-Exposure to Borrelia burgdorferi s.l. and Anaplasma phagocytophilum: Unraveling the Hematological Profile in Sheep" Life 13, no. 2: 469. https://doi.org/10.3390/life13020469