Comparative Transcriptome Analysis Provides Insights into the Molecular Mechanism Underlying the Effect of MeJA Treatment on the Biosynthesis of Saikosaponins in Bupleurum chinense DC.
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Material and MeJA Treatment
2.2. RNA Isolation, Library Construction, and Transcriptome Sequencing
2.3. Transcriptome Assembly and Annotation
2.4. Differential Expression Analysis and Functional Enrichment
2.5. Simple Sequence Repeats (SSRs) and Variant Detection
2.6. Quantitative Real-Time PCR (qRT–PCR) Analysis
2.7. HPLC Analysis
2.8. Statistical Analysis
3. Results
3.1. Transcriptome Sequencing and De Novo Assembly
3.2. Functional Annotation and Classification
3.3. Identification of DEGs
3.4. Enrichment Analysis of DEGs
3.5. DEGs Involved in SS Biosynthetic Pathways
3.6. Identification of TFs
3.7. Integrated Analysis
3.8. Identification of SSRs and Variant Detection
3.9. qRT–PCR Validation
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Sample Name | Clean Reads | Clean Bases (bp) | Clean Reads (%) | Clean GC (%) | Clean Q20 (%) | Clean Q30 (%) | rRNA Ratio (%) | Mapped Ratio (%) |
---|---|---|---|---|---|---|---|---|
CKR1 | 44,526,918 | 6,679,037,700 | 99.65 | 43.01 | 97.20 | 92.51 | 0.35 | 75.9 |
CKR2 | 42,887,626 | 6,433,143,900 | 99.71 | 42.87 | 97.22 | 92.72 | 0.29 | 75.83 |
CKR3 | 53,660,932 | 8,049,139,800 | 99.75 | 42.83 | 97.26 | 92.94 | 0.25 | 74.66 |
M6HR1 | 42,239,902 | 6,335,985,300 | 99.67 | 42.88 | 96.87 | 91.99 | 0.33 | 76.9 |
M6HR2 | 54,916,582 | 8,237,487,300 | 99.66 | 43.12 | 97.05 | 92.11 | 0.34 | 76.02 |
M6HR3 | 57,836,632 | 8,675,494,800 | 99.68 | 43.03 | 97.04 | 92.26 | 0.32 | 76.41 |
M12HR1 | 45,061,582 | 6,759,237,300 | 99.58 | 42.78 | 96.89 | 92.07 | 0.42 | 76.51 |
M12HR2 | 54,093,938 | 8,114,090,700 | 99.54 | 42.92 | 97.19 | 92.58 | 0.46 | 76.85 |
M12HR3 | 56,753,582 | 8,513,037,300 | 99.55 | 42.88 | 96.82 | 91.87 | 0.45 | 76.78 |
M24HR1 | 50,885,024 | 7,632,753,600 | 99.57 | 42.84 | 97.37 | 93.13 | 0.43 | 76.06 |
M24HR2 | 54,550,916 | 8,182,637,400 | 99.68 | 42.85 | 96.73 | 91.70 | 0.32 | 76.53 |
M24HR3 | 65,380,004 | 9,807,000,600 | 99.60 | 42.72 | 97.29 | 92.95 | 0.40 | 75.59 |
M48HR1 | 45,675,820 | 6,851,373,000 | 99.69 | 42.93 | 97.24 | 92.84 | 0.31 | 77.19 |
M48HR2 | 51,196,014 | 7,679,402,100 | 99.64 | 42.96 | 97.23 | 92.83 | 0.36 | 75.92 |
M48HR3 | 60,980,926 | 9,147,138,900 | 99.52 | 42.96 | 97.34 | 92.96 | 0.48 | 76.38 |
M72HR1 | 48,107,866 | 7,216,179,900 | 99.50 | 43.08 | 95.98 | 89.99 | 0.50 | 75.8 |
M72HR2 | 48,412,486 | 7,261,872,900 | 99.65 | 42.79 | 97.33 | 92.76 | 0.35 | 76.52 |
M72HR3 | 55,104,860 | 8,265,729,000 | 99.55 | 43.05 | 97.15 | 92.69 | 0.45 | 77.62 |
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Mao, Y.; Yang, Y.; Li, Y.; Zhang, Y.; Wei, P.; Chen, H.; Hou, D. Comparative Transcriptome Analysis Provides Insights into the Molecular Mechanism Underlying the Effect of MeJA Treatment on the Biosynthesis of Saikosaponins in Bupleurum chinense DC. Life 2023, 13, 563. https://doi.org/10.3390/life13020563
Mao Y, Yang Y, Li Y, Zhang Y, Wei P, Chen H, Hou D. Comparative Transcriptome Analysis Provides Insights into the Molecular Mechanism Underlying the Effect of MeJA Treatment on the Biosynthesis of Saikosaponins in Bupleurum chinense DC. Life. 2023; 13(2):563. https://doi.org/10.3390/life13020563
Chicago/Turabian StyleMao, Yanping, Yuping Yang, Yuchan Li, Yiguan Zhang, Ping Wei, Hua Chen, and Dabin Hou. 2023. "Comparative Transcriptome Analysis Provides Insights into the Molecular Mechanism Underlying the Effect of MeJA Treatment on the Biosynthesis of Saikosaponins in Bupleurum chinense DC." Life 13, no. 2: 563. https://doi.org/10.3390/life13020563