Comparing Methodologies for Stomatal Analyses in the Context of Elevated Modern CO2
Abstract
:1. Introduction
2. Materials and Methods
2.1. Specimen Sampling and Preparation
2.2. Microscopy
2.3. C Isotope Measurements
2.4. Gas Exchange Model
3. Results
3.1. Method Comparisons
3.2. CO2 Reconstructions Using Both Methods
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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n | Average Stomata Density (Stomata m−2) | Standard Deviation Stomata Density (Stomata m−2) | Average Guard Cell Length (m) | Guard Cell Length Standard Deviation (m) | Average Guard Cell Width (m) | Guard Cell Width Standard Deviation (m) | |
---|---|---|---|---|---|---|---|
Populus tremuloides impressions | 82 | 1.71 × 108 | 5.95 × 107 | 2.58 × 10−5 | 6.07 × 10−6 | 7.56 × 10−6 | 2.78 × 10−6 |
Thuja impressions | 24 | 2.60 × 108 | 8.92 × 107 | 3.17 × 10−5 | 7.77 × 10−6 | 1.34 × 10−5 | 4.50 × 10−6 |
All impressions | 106 | 1.91 × 108 | 7.68 × 107 | 2.71 × 10−5 | 6.91 × 10−6 | 8.92 × 10−6 | 4.05 × 10−6 |
CrO3-cleared Thuja | 10 | 2.46 × 108 | 9.67 × 107 | 2.98 × 10−5 | 4.35 × 10−6 | 9.01 × 10−6 | 4.36 × 10−6 |
NaOH-cleared Populus | 20 | 1.41 × 108 | 5.10 × 107 | 3.55 × 10−5 | 7.04 × 10−6 | 2.12 × 10−5 | 3.61 × 10−6 |
All cleared leaves | 30 | 1.76 × 108 | 8.46 × 107 | 3.36 × 10−5 | 6.76 × 10−6 | 1.72 × 10−5 | 6.98 × 10−6 |
Stomatal Density | Populus tremuloides | Thuja occidentalis |
---|---|---|
Populus tremuloides | ||
Thuja occidentalis | <0.01 | |
Thuja plicata | <0.01 | 0.13 |
Guard Cell Length | Populus tremuloides | Thuja occidentalis |
Populus tremuloides | ||
Thuja occidentalis | 0.13 | |
Thuja plicata | <0.01 | 0.56 |
Guard Cell Width | Populus tremuloides | Thuja occidentalis |
Populus tremuloides | ||
Thuja occidentalis | <0.01 | |
Thuja plicata | <0.01 | 0.41 |
Value | Stomatal Density (Stomata m−2) | Guard Cell Length (m) | Guard Cell Width (m) |
---|---|---|---|
Mean for impressions | 1.74 × 10 8 | 2.97 × 10−5 | 9.95 × 10−6 |
Mean for cleared leaves | 1.70 × 108 | 3.37 × 10−5 | 1.72 × 10−5 |
p-value | 0.83 | 0.05 | <0.001 |
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Stein, R.A.; Sheldon, N.D.; Smith, S.Y. Comparing Methodologies for Stomatal Analyses in the Context of Elevated Modern CO2. Life 2024, 14, 78. https://doi.org/10.3390/life14010078
Stein RA, Sheldon ND, Smith SY. Comparing Methodologies for Stomatal Analyses in the Context of Elevated Modern CO2. Life. 2024; 14(1):78. https://doi.org/10.3390/life14010078
Chicago/Turabian StyleStein, Rebekah A., Nathan D. Sheldon, and Selena Y. Smith. 2024. "Comparing Methodologies for Stomatal Analyses in the Context of Elevated Modern CO2" Life 14, no. 1: 78. https://doi.org/10.3390/life14010078