Growth Characteristics of a Desmodesmus Species from the San Antonio Springs and Its Short-Term Impact on Soil Microbial Dynamics
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals, Culture Media, and Reference Algae
2.2. General Procedures for Algae Culturing, Harvest, and Quantitation
2.3. Isolation of Xenic and Axenic Cultures
2.4. Species Identification and Phylogenetic Analysis
2.5. Growth Rate Determination
2.6. Biomass Processing and Estimation of Carbohydrates, Proteins, and Lipids
2.7. Pigment Extraction and Analyses
2.8. Salt and Temperature Tolerance Experiments
2.9. Soil Treatment and Biodiversity Analysis
2.10. Statistical Analysis
3. Results
3.1. Isolation and Identification of a Desmodesmus Species from the Blue Hole
3.2. Growth Characteristics and Biomass Composition
3.3. Pigment Profiles
3.4. Salt and Temperature Tolerance
3.5. Soil Studies
3.5.1. Soil Persistence
3.5.2. Soil Microbial Activity and Functional Diversity
4. Discussion
4.1. Morphological Identification and Phylogenetic Analysis
4.2. Growth Characteristics
4.3. Salt and Temperature Tolerance
4.4. Compositional Biomass Analysis
4.5. Soil Persistence and Impact on Soil Microbiome
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Growth or Biomass Parameter | Desmodesmus XB | Desmodesmus AxB |
---|---|---|
Specific growth rate m (d−1) | 0.46 ± 0.042 | 0.77 ± 0.03 |
Doubling time (d) | 1.53 + 0.069 | 1.02 ± 0.15 |
Cell number (×106 cells/mL) | 17.6 ± 4.2 | 29.6 ± 6.1 |
Dry weight (DW, mg/L) | 441.3 ± 72.0 | 488.7 ± 81.9 |
Carbohydrate (% DW) | 21 ± 6.3 | 13 ± 4.1 |
Protein (% DW) | 50 ± 0.9 | 49 ± 0.4 |
Lipid (% DW) | 32 ± 3.4 | 28 ± 0.3 |
Chlorophylls (mg/L) * | 11.7 [11.4] | 13.8 [14.0] |
Carotenoids (mg/L) * | 2.6 [1.1] | 2.2 [0.9] |
Parameter | Soil Treatment | September–October (Fall) | January–March (Spring) |
---|---|---|---|
AWCD (120 h) | Water | 1.66 ± 0.03 | 1.46 ± 0.09 |
Spent medium | 2.09 ± 0.12 | 2.32 ± 0.16 | |
XB cells | 3.96 ± 0.24 | 3.26 ± 0.17 | |
Shannon diversity index (120 h) | Water | 2.90 ± 0.11 | 2.92 ± 0.06 |
Spent medium | 3.00 ± 0.07 | 2.91 ± 0.07 | |
XB cells | 3.06 ± 0.05 | 2.97 ± 0.07 |
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Bomer, L.K.; Leverett, B.D. Growth Characteristics of a Desmodesmus Species from the San Antonio Springs and Its Short-Term Impact on Soil Microbial Dynamics. Life 2024, 14, 1053. https://doi.org/10.3390/life14091053
Bomer LK, Leverett BD. Growth Characteristics of a Desmodesmus Species from the San Antonio Springs and Its Short-Term Impact on Soil Microbial Dynamics. Life. 2024; 14(9):1053. https://doi.org/10.3390/life14091053
Chicago/Turabian StyleBomer, Lauren K., and Betsy D. Leverett. 2024. "Growth Characteristics of a Desmodesmus Species from the San Antonio Springs and Its Short-Term Impact on Soil Microbial Dynamics" Life 14, no. 9: 1053. https://doi.org/10.3390/life14091053