The Hepatoprotective Effects of Camellia sinensis on Cisplatin-Induced Acute Liver Injury
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals
2.2. The Experimental Animals and Study Groups
2.3. The Procurement and Preparation of White Tea
2.4. Blood and Liver Tissues Collection
2.5. Biochemical Analysis
2.5.1. Determination of Serum ALT and AST Levels
2.5.2. Determination of Tissue MDA and GSH Levels
2.6. Histopathological Analysis
2.7. Immunohistochemical Analysis (IHC)
2.8. Statistical Analysis
3. Results
3.1. Biochemical Results
3.2. Histopathological and Immunohistochemical Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Component | Amount in Dry Weight |
---|---|
Gallic acid | 0.13 (%) |
Caffeine | 5.62 (%) |
EGC (epigallocatechin) | 2.54 (%) |
EC (epicatechin) | 0.91 (%) |
EGCG (epigallocatechin gallate) | 10.39 (%) |
ECG (epicatechin gallate) | 3.58 (%) |
Copper | 0.08 (ppm) |
Iron | 0.12 (ppm) |
Zinc | 0.54 (ppm) |
Sodium | 2.87 (ppm) |
Potassium | 283.00 (ppm) |
Calcium | 6.12 (ppm) |
Magnesium | 26.88 (ppm) |
Aluminum | 0.98 (ppm) |
MDA (nmol/g Tissue) | GSH (µmol/g Tissue) | |
---|---|---|
Control | 1.17 ± 0.11 | 14.90 ± 2.04 |
Cisplatin | 1.56 ± 0.26 a,* | 16.74 ± 2.00 a,** |
Cisplatin + White Tea | 1.39 ± 0.39 | 15.08 ± 1.38 b,* |
AST (U/L) | ALT (U/L) | |
---|---|---|
Control | 71.13 ± 4.49 | 35.25 ± 4.89 |
Cisplatin | 97.88 ± 7.20 a,** | 43.75 ± 7.61 a,* |
Cisplatin + White Tea | 77.00 ± 6.21 | 40.38 ± 3.89 |
Score | Finding |
---|---|
0 | Less than 5% caspase-3 positive hepatocytes |
1 | Less than 25% caspase-3 positive hepatocytes |
2 | Less than 50% caspase-3 positive hepatocytes |
3 | More than 50% caspase-3 positive hepatocytes |
Group | Caspase-3 Positivity Score |
---|---|
Control | 0.00 ± 0.35 |
Cisplatin | 3.00 ± 0.46 a |
Cisplatin+ White tea | 1.00 ± 0.46 b |
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Yilmaz, A.; Dizman, F.; Akyildiz, K.; Mataraci Karakas, S.; Mercantepe, T.; Uydu, H.A.; Tumkaya, L.; Ozturk, K. The Hepatoprotective Effects of Camellia sinensis on Cisplatin-Induced Acute Liver Injury. Life 2024, 14, 1077. https://doi.org/10.3390/life14091077
Yilmaz A, Dizman F, Akyildiz K, Mataraci Karakas S, Mercantepe T, Uydu HA, Tumkaya L, Ozturk K. The Hepatoprotective Effects of Camellia sinensis on Cisplatin-Induced Acute Liver Injury. Life. 2024; 14(9):1077. https://doi.org/10.3390/life14091077
Chicago/Turabian StyleYilmaz, Adnan, Fatih Dizman, Kerimali Akyildiz, Sibel Mataraci Karakas, Tolga Mercantepe, Huseyin Avni Uydu, Levent Tumkaya, and Koksal Ozturk. 2024. "The Hepatoprotective Effects of Camellia sinensis on Cisplatin-Induced Acute Liver Injury" Life 14, no. 9: 1077. https://doi.org/10.3390/life14091077