Methods for Screening and Isolating Extremely Heat-Resistant Escherichia coli from Meat Sources
Abstract
:1. Introduction
2. Materials and Methods
2.1. Samples and Growth Conditions
2.2. Multiplex PCR to Screen for tLST
2.3. Aluminum Heat-Block Method for Screening and Isolation of XHR Bacteria
2.4. Aluminum Heat-Block Method for Screening of XHR Phenotype in E. coli Isolates and Identification of Heat-Resistance Magnitude
2.5. Water-Bath Method for Screening of XHR Phenotype in E. coli Isolates
3. Results
3.1. Screening of Extreme Heat Resistance Using an Aluminum Heat Block
3.1.1. Heat Resistance in Cattle Fecal Samples
3.1.2. Heat Resistance among E. coli Isolated from Meat Sources
3.2. Heat Resistance Identified among E. coli Isolated from the Beef Processing Continuum Using the Refined 60 °C Water Bath Method
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Primer | Sequence | Amplicon Size (bp) | Reference |
---|---|---|---|
1266 F 1372 R a | 5′-AATGCAGGCGGTGATGAAGA-3′ 5′-CGCTGATTGCCCATCAACAG-3′ | 107 | [26] |
4295 F 4505 R a | 5′-CGAGGGAGAATTCCAGTCCG-3′ 5′-GGCACTACGCTAATCCTGCT-3′ | 210 | [26] |
7069 F 7404 R a | 5′-CTCATTGGATGCTTCGCTGC-3′ 5′-ACGGAAACCATTGAGGCGAT-3′ | 335 | [26] |
14160 F 14699 R a | 5′-CCTGGCATTGTTTTCTGGCC-3′ 5′-GGCTGTTCGATGACGCATTC-3′ | 539 | [26] |
ClpK2F ClpK2R | 5′-ACGATCACTATCGCCAACTG-3′ 5′-AGTATTTATCCAGCTCGGGCGTG-3′ | 711 | [26] |
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Guragain, M.; Smith, G.E.; Bosilevac, J.M. Methods for Screening and Isolating Extremely Heat-Resistant Escherichia coli from Meat Sources. Life 2024, 14, 1123. https://doi.org/10.3390/life14091123
Guragain M, Smith GE, Bosilevac JM. Methods for Screening and Isolating Extremely Heat-Resistant Escherichia coli from Meat Sources. Life. 2024; 14(9):1123. https://doi.org/10.3390/life14091123
Chicago/Turabian StyleGuragain, Manita, Gregory E. Smith, and Joseph M. Bosilevac. 2024. "Methods for Screening and Isolating Extremely Heat-Resistant Escherichia coli from Meat Sources" Life 14, no. 9: 1123. https://doi.org/10.3390/life14091123