The RUBISCO to Photosystem II Ratio Limits the Maximum Photosynthetic Rate in Picocyanobacteria
Abstract
:1. Introduction
2. Experimental Section
2.1. Culturing
2.2. Whole Cell Protein Extraction and Quantitative Immunoblotting
2.3. Physiological Measurements
3. Results and Discussion
3.1. Changes in the Photosynthetic Electron Transport Chain Stoichiometry Following Subculturing
3.2. Molar Quantitations of Photosynthetic Protein Complexes
Parameter Measured | MIT 9313 | MED 4 | WH 8102 |
---|---|---|---|
σPSII (Å2PSII−1) | 340 ± 17 | 312 ± 16 | 307 ± 14 |
ETR Capacity (e-PSII−1s−1) | 155 ± 3 | 113 ± 12 | 336 ± 44 |
ETRmax (e-PSII−1s−1) | 102 ± 2 | 98 ± 3 | 238 ± 5 |
EK (umol photons m−2·s−1) | 54 ± 3 | 67 ± 4 | 154 ± 5 |
μ (day−1) | 0.25 ± 0.03 | 0.19 ± 0.02 | 0.24 ± 0.02 |
PSI:PSII | 9 ± 5 | 2 ± 0.7 | 2 ± 0.2 |
Cytb6f:PSII | 2.5 ± 0.5 | 1.3 ± 0.4 | 1.1 ± 0.1 |
Cytb6f:PSI | 0.2 ± 0.2 | 0.7 ± 0.1 | 0.5 ± 0.1 |
RUBISCO:PSII | 0.5 ± 0.1 | 0.8 ± 0.2 | 1.5 ± 0.2 |
3.3. Photosystem II Photophysiological and Electron Transport Differences among Strains
3.4. Discussion
4. Conclusions
Acknowledgments
Author Contributions
Supplementary Materials
Conflicts of Interest
References
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Zorz, J.K.; Allanach, J.R.; Murphy, C.D.; Roodvoets, M.S.; Campbell, D.A.; Cockshutt, A.M. The RUBISCO to Photosystem II Ratio Limits the Maximum Photosynthetic Rate in Picocyanobacteria. Life 2015, 5, 403-417. https://doi.org/10.3390/life5010403
Zorz JK, Allanach JR, Murphy CD, Roodvoets MS, Campbell DA, Cockshutt AM. The RUBISCO to Photosystem II Ratio Limits the Maximum Photosynthetic Rate in Picocyanobacteria. Life. 2015; 5(1):403-417. https://doi.org/10.3390/life5010403
Chicago/Turabian StyleZorz, Jackie K., Jessica R. Allanach, Cole D. Murphy, Mitchell S. Roodvoets, Douglas A. Campbell, and Amanda M. Cockshutt. 2015. "The RUBISCO to Photosystem II Ratio Limits the Maximum Photosynthetic Rate in Picocyanobacteria" Life 5, no. 1: 403-417. https://doi.org/10.3390/life5010403