LINC00084/miR-204/ZEB1 Axis Mediates Myofibroblastic Differentiation Activity in Fibrotic Buccal Mucosa Fibroblasts: Therapeutic Target for Oral Submucous Fibrosis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture
2.2. Reagents
2.3. Tissue Acquisition
2.4. Quantitative Real-Time PCR and Western Blot Analysis
2.5. Lentiviral-Mediated Knockdown or Overexpression of LINC00084
2.6. Collagen Gel Contraction
2.7. Cell Migration and Invasion Assays
2.8. Dual-Luciferase Reporter Gene Assay
2.9. RIP (RNA Immunoprecipitation) Assay
2.10. Statistical Analysis
3. Results
3.1. Arecoline-Induced LINC00084 Promotes Myofibroblast Activation
3.2. The Upregulated LINC00084 in OSF Tissues Is Positively Associated with Several Fibrosis Markers
3.3. Silencing LINC00084 in fBMFs Mitigates the Myofibroblasts Features
3.4. LINC00084 Acts as a Molecular Sponge of miR-204
3.5. MiR-204 Regulates the Myofibroblast Activities by Directly Targeting ZEB1
3.6. LINC00084 Mediates the Myofibroblast Activation via Sponge Regulation of miR-204-ZEB1 Interaction
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Conflicts of Interest
References
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Lee, Y.-H.; Liao, Y.-W.; Lu, M.-Y.; Hsieh, P.-L.; Yu, C.-C. LINC00084/miR-204/ZEB1 Axis Mediates Myofibroblastic Differentiation Activity in Fibrotic Buccal Mucosa Fibroblasts: Therapeutic Target for Oral Submucous Fibrosis. J. Pers. Med. 2021, 11, 707. https://doi.org/10.3390/jpm11080707
Lee Y-H, Liao Y-W, Lu M-Y, Hsieh P-L, Yu C-C. LINC00084/miR-204/ZEB1 Axis Mediates Myofibroblastic Differentiation Activity in Fibrotic Buccal Mucosa Fibroblasts: Therapeutic Target for Oral Submucous Fibrosis. Journal of Personalized Medicine. 2021; 11(8):707. https://doi.org/10.3390/jpm11080707
Chicago/Turabian StyleLee, Yu-Hsien, Yi-Wen Liao, Ming-Yi Lu, Pei-Ling Hsieh, and Cheng-Chia Yu. 2021. "LINC00084/miR-204/ZEB1 Axis Mediates Myofibroblastic Differentiation Activity in Fibrotic Buccal Mucosa Fibroblasts: Therapeutic Target for Oral Submucous Fibrosis" Journal of Personalized Medicine 11, no. 8: 707. https://doi.org/10.3390/jpm11080707