A Clip Domain Serine Protease Involved in Egg Production in Nilaparvata lugens: Expression Patterns and RNA Interference
Abstract
:1. Introduction
2. Materials and Methods
2.1. Insect Rearing
2.2. Cloning the cDNA of NlPCE3
2.3. Sequence Comparison and Phylogenetic Analysis
2.4. Real-time Quantitative PCR Analysis
2.5. RNA Interference
2.6. Dissection Observation and Fertility Analysis
2.7. Immunofluorescence
3. Results
3.1. Identification and Phylogenetic Analysis of NlPCE3
3.2. Developmental and Tissue-specific Expression of NlPCE3
3.3. Effects of RNA Interference
3.4. Immunofluorescence Analysis of NlPCE3 Expression
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Primers | Primer Sequence (5′–3′) |
---|---|
for cloning cDNA | |
NlPCE3-F | CGGCAGTCTGCTTCAGTTT |
NlPCE3-R | GTCGAAAACTAGGTCAAACAT |
for qRT-PCR | |
NlPCE3-qF | CGAAATGGAAGATTGCTGAGTC |
NlPCE3-qR | TGGTGTTGGCGTTGATTATGG |
Nl18S-qF | GTAACCCGCTGAACCTCC |
Nl18S-qR | GTCCGAAGACCTCACTAAATCA |
for synthesizing dsRNA | |
T7-NlPCE3-dsF | GGATCCTAATACGACTCACTATAGGGACTCTCGTTTCCAGATACC |
T7-NlPCE3-dsR | GGATCCTAATACGACTCACTATAGGCACCAGTGACTTCGCTC |
T7-GFP-dsF | GGATCCTAATACGACTCACTATAGGGATACGTGCAGGAGAGGAC |
T7-GFP-dsR | GGATCCTAATACGACTCACTATAGGGCAGATTGTGTGGACAGG |
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Wu, J.-m.; Zheng, R.-e.; Zhang, R.-j.; Ji, J.-l.; Yu, X.-p.; Xu, Y.-p. A Clip Domain Serine Protease Involved in Egg Production in Nilaparvata lugens: Expression Patterns and RNA Interference. Insects 2019, 10, 378. https://doi.org/10.3390/insects10110378
Wu J-m, Zheng R-e, Zhang R-j, Ji J-l, Yu X-p, Xu Y-p. A Clip Domain Serine Protease Involved in Egg Production in Nilaparvata lugens: Expression Patterns and RNA Interference. Insects. 2019; 10(11):378. https://doi.org/10.3390/insects10110378
Chicago/Turabian StyleWu, Jia-min, Rong-er Zheng, Rui-juan Zhang, Jin-liang Ji, Xiao-ping Yu, and Yi-peng Xu. 2019. "A Clip Domain Serine Protease Involved in Egg Production in Nilaparvata lugens: Expression Patterns and RNA Interference" Insects 10, no. 11: 378. https://doi.org/10.3390/insects10110378