Morphological and Molecular Analysis of Australian Earwigs (Dermaptera) Points to Unique Species and Regional Endemism in the Anisolabididae Family
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sampling, Identification, and Morphology
2.2. Barcoding
2.3. Phylogeny
3. Results
3.1. Sampling, Identification, and Morphology
3.2. Barcoding
3.3. Phylogeny
4. Discussion
4.1. The Utility of Barcoding for Earwigs
4.2. Distribution of Anisolabididae
4.3. Conflict between Morphological and Molecular Inferences
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Appendix A. Key to the Anisolabididae Morphospecies of the Southern Australian Grain Belt
- 1
- Forceps rounded, teeth may be present, lifting of penultimate ventral sternite reveals parameres lying within the body cavity, although they may be anteriorly recessed………………………………………………………………………………………………………………………………………..…....…..3
- 2
- Forceps straight, short, no true teeth but the medial surface may be slightly ridged, no parameres present……………………………………………………………………………………………………………………………………………………………….....15
- 3
- Patterned/banded on dorsal surface, mixture of red, yellow, brown; medial forceps tooth on inner margins of both forceps near base…………………………………………………………………………………………………………………………….………………....…Anisolabis sp. 1
- 4
- No patterning, uniform colour or otherwise continuous gradient from light brown to dark brown/black, forceps teeth may be present, if so are dorsoventrally oriented and protruding from the dorsal surface near the base………………………………………..……………………………………5
- 5
- From South Australia; body length including forceps generally lesser than 15 mm; forceps roughly as wide as long………………………………………………………………………………………………………………………..…………………………………......……7
- 6
- From Western Australia; body length including forceps generally greater than 15 mm; forceps longer than wide…………………………………………………………………………………………………………………...…………….……………….……………....13
- 7
- Forceps roughly as wide as long and curving upward, large dorsoventrally oriented teeth on dorsal surface near the base of the forceps, parameres roughly twice as long as wide…………………………………...……………....…………………………………………….Gonolabis nr. gilesi
- 8
- Forceps roughly as wide as long and not curving upward, no teeth, parameres roughly 1.5 times as long as wide………………………………………………………………………………………………...…………………………………………………………….…..9
- 9
- Apical angle of paramere sharp and clearly acute……………………………………………………………………………………….Gonolabis forcipata
- 10
- Apical angle of paramere rounded or with a slight bump which protrudes from the external margin.……………………………………………………………………………………………………..………………………………………………………11
- 11
- Angle of external paramere margin more or less straight but with concavity along the anterior edge of the angle……………………………………………………………………………………………………………………………………....……….Gonolabis sp. 2
- 12
- Angle of external paramere margin rounded, external apical margin with obtuse-angled bump which is proceeds straight down on the internal apical margin………….…………………………………………………………………………………………………………………………..Gonolabis sp. 3
- 13
- Lateral internal edges of forceps with an almost right-angled corner near the anterior end with a roughly parallel region, thoracic sternites as dark as dorsal surface in places; femur uniformly brown or with a proximal light patch, parameres much longer than wide with no curvature on outer edges and acuminate tip……………………………………………………………………………………………………………...Anisolabis sp. 2
- 14
- No parallel region near the base of the forceps, lateral internal edge smoothly curved (with small ridges), viewed from the side a triangular ridge is apparent near the posterior end of the forceps but this is not a pronounced tooth, parameres as long as wide and slightly pinched toward the tip forming a kind of snout……………………………………………………………………………..………..………………..Gonolabis sp. 1
- 15
- Patterned/banded on dorsal surface, especially the head; mixture of red, yellow, brown, red; lateral forceps tooth on inner margin near base…………………………………………………………………………………………………………………………………....…………...Anisolabis sp. 1
- 16
- No patterning; uniform colour or otherwise gradient from light to dark; small bumps on internal margins of forceps but no true teeth……………………………………………………………………………………………………………………………………………………..…………17
- 17
- From South Australia; body length including forceps generally lesser than 15 mm………………………………………………………………….....19
- 18
- From Western Australia; body length including forceps generally greater than 15 mm………………………………………………………….….....21
- 19
- Small dorsal bump near attachment of forceps, not a true tooth but distinct……………………………………………………….Gonolabis nr. gilesi
- 20
- No dorsal bump near attachment of forceps, not to be confused with the lateral ridge where the forceps slide under the ultimate tergite at the attachment site……………………………………………………………………………………………………………………………..Gonolabis forcipata
- 21
- Forceps clearly curved upwards near tip; setae on final sternite very long; small projections on posterior edge of final dorsal tergite that align with forceps attachments creating straight edge between forceps attachment; thoracic sternites as dark as dorsal surface in places; femur uniformly brown or with a proximal light patch………………………………………………………………………..……….…………...Gonolabis sp. 1
- 22
- Forceps more or less straight towards tip; final sternite glabrous or covered in moderate length setae; pronounced projections on posterior edge of final dorsal tergite that align with forceps creating concave edge between forceps attachment; thoracic sternites lighter than dorsal surface; femur as dark as dorsal surface near trochanter and lighter towards tibia………………………………………………….....Anisolabis sp. 2
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PC1 | PC2 | PC3 | |
---|---|---|---|
Component Loadings | |||
Basal width of forceps | 0.370 | −0.587 | 0.593 |
Forceps asymmetry index | 0.733 | 0.017 | 0.026 |
Paramere length-width ratio | 0.417 | 0.757 | 0.163 |
Forceps length-width ratio | −0.389 | 0.285 | 0.788 |
Eigenvalues | |||
Standard deviation | 1.292 | 1.051 | 1.003 |
Variance explained | 0.417 | 0.276 | 0.252 |
Cumulative variance explained | 0.417 | 0.693 | 0.945 |
N | Forficula auricularia | Labidura truncata | Nala lividipes | Anisolabis sp. 1 | Anisolabis sp. 2 | Gonolabis forcipata | Gonolabis nr. gilesi | Gonolabis sp. 1 | Gonolabis sp. 2 | Gonolabis sp. 3 | |
---|---|---|---|---|---|---|---|---|---|---|---|
Forficula auricularia | 39 | 0.009 (0.003) | 0.015 | 0.016 | 0.015 | 0.016 | 0.016 | 0.015 | 0.015 | 0.015 | 0.015 |
Labidura truncata | 33 | 0.222 | 0.007 (0.002) | 0.014 | 0.014 | 0.015 | 0.015 | 0.015 | 0.014 | 0.014 | 0.015 |
Nala lividipes | 26 | 0.227 | 0.181 | 0.005 (0.001) | 0.014 | 0.015 | 0.015 | 0.015 | 0.014 | 0.015 | 0.016 |
Anisolabis sp. 1 | 17 | 0.220 | 0.178 | 0.194 | 0.008 (0.002) | 0.012 | 0.013 | 0.013 | 0.012 | 0.013 | 0.013 |
Anisolabis sp. 2 | 5 | 0.228 | 0.200 | 0.200 | 0.105 | 0.001 (0.001) | 0.013 | 0.013 | 0.012 | 0.013 | 0.015 |
Gonolabis forcipata | 8 | 0.226 | 0.209 | 0.199 | 0.145 | 0.154 | 0.017 (0.003) | 0.014 | 0.013 | 0.014 | 0.015 |
Gonolabis nr. gilesi | 3 | 0.222 | 0.198 | 0.202 | 0.136 | 0.139 | 0.179 | 0.002 (0.001) | 0.011 | 0.013 | 0.014 |
Gonolabis sp. 1 | 12 | 0.216 | 0.189 | 0.196 | 0.117 | 0.121 | 0.151 | 0.104 | 0.040 (0.007) | 0.011 | 0.014 |
Gonolabis sp. 2 | 4 | 0.217 | 0.196 | 0.214 | 0.151 | 0.154 | 0.178 | 0.151 | 0.115 | 0.013 (0.002) | 0.013 |
Gonolabis sp. 3 | 2 | 0.238 | 0.199 | 0.221 | 0.139 | 0.162 | 0.182 | 0.195 | 0.169 | 0.166 | 0.000 (0.000) |
N | Anisolabis sp. 1 | Anisolabis sp. 2 | Gonolabis forcipata | Gonolabis nr. gilesi | Gonolabis sp. 1 | Gonolabis sp. 2 | Gonolabis sp. 3 | |
---|---|---|---|---|---|---|---|---|
Anisolabis sp. 1 | 11 | 0.016 (0.004) | 0.019 | 0.022 | 0.021 | 0.020 | 0.023 | 0.022 |
Anisolabis sp. 2 | 5 | 0.186 | 0.001 (0.001) | 0.022 | 0.020 | 0.020 | 0.022 | 0.023 |
Gonolabis forcipata | 8 | 0.249 | 0.267 | 0.020 (0.005) | 0.022 | 0.022 | 0.023 | 0.024 |
Gonolabis nr. gilesi | 3 | 0.230 | 0.214 | 0.254 | 0.001 (0.001) | 0.014 | 0.021 | 0.022 |
Gonolabis sp. 1 | 16 | 0.221 | 0.194 | 0.268 | 0.106 | 0.054 (0.012) | 0.020 | 0.021 |
Gonolabis sp. 2 | 5 | 0.282 | 0.258 | 0.305 | 0.221 | 0.217 | 0.019 (0.004) | 0.023 |
Gonolabis sp. 3 | 2 | 0.234 | 0.262 | 0.315 | 0.265 | 0.252 | 0.294 | 0.004 (0.003) |
Individual | Morphospecies | Family | cox1 | cob | 28S | tuba1 |
---|---|---|---|---|---|---|
167 | Anisolabis sp. 1 | Anisolabididae | MK399508 | MK399441 | MK399637 | - |
173 | Anisolabididae | MK399511 | MK399442 | MK399638 | - | |
253 | Anisolabididae | MK399540 | MK399447 | MK399643 | - | |
254 | Anisolabididae | MK399541 | MK399448 | MK399644 | MK399671 | |
269 | Anisolabididae | MK399545 | MK399449 | - | MK399672 | |
298 | Anisolabididae | MK399554 | - | - | MK399675 | |
301 | Anisolabididae | MK399558 | MK399453 | - | - | |
306 | Anisolabididae | MK399562 | MK399454 | - | - | |
344 | Anisolabididae | MK399584 | MK399464 | MK399652 | - | |
347 | Anisolabididae | MK399587 | MK399465 | MK399653 | - | |
348 | Anisolabididae | MK399588 | MK399466 | MK399654 | - | |
153 | Anisolabis sp. 2 | Anisolabididae | MK399502 | MK399435 | - | MK399665 |
155 | Anisolabididae | MK399504 | MK399437 | - | MK399667 | |
160 | Anisolabididae | MK399505 | MK399438 | MK399635 | - | |
161 | Anisolabididae | MK399506 | MK399439 | MK399636 | MK399668 | |
288 | Gonolabis forcipata | Anisolabididae | MK399548 | MK399452 | - | MK399674 |
324 | Anisolabididae | MK399574 | MK399458 | - | MK399678 | |
326 | Anisolabididae | MK399575 | MK399459 | MK399649 | MK399679 | |
327 | Anisolabididae | MK399576 | MK399460 | MK399650 | - | |
331 | Anisolabididae | MK399578 | MK399462 | - | - | |
333 | Anisolabididae | MK399579 | MK399463 | - | MK399681 | |
308 | Gonolabis nr. gilesi | Anisolabididae | MK399564 | MK399456 | MK399647 | MK399677 |
309 | Anisolabididae | MK399565 | MK399457 | MK399648 | - | |
484 | Anisolabididae | MK399610 | MK399471 | MK399658 | - | |
146 | Gonolabis sp. 1 | Anisolabididae | MK399500 | MK399433 | MK399632 | - |
152 | Anisolabididae | MK399501 | MK399434 | MK399633 | - | |
384 | Anisolabididae | MK399598 | MK399467 | MK399655 | MK399682 | |
385 | Anisolabididae | MK399599 | MK399468 | MK399656 | MK399683 | |
386 | Anisolabididae | MK399600 | MK399469 | MK399657 | - | |
533 | Anisolabididae | MK399613 | MK399473 | MK399659 | MK399684 | |
535 | Anisolabididae | MK399614 | MK399475 | MK399660 | - | |
536 | Anisolabididae | MK399615 | MK399476 | MK399661 | MK399685 | |
539 | Anisolabididae | MK399618 | MK399479 | MK399664 | MK399688 | |
232 | Gonolabis sp. 2 | Anisolabididae | MK399531 | MK399443 | MK399639 | - |
236 | Anisolabididae | MK399532 | MK399444 | MK399640 | MK399669 | |
239 | Anisolabididae | MK399533 | MK399445 | MK399641 | - | |
274 | Anisolabididae | MK399546 | MK399450 | - | - | |
251 | Gonolabis sp. 3 | Anisolabididae | MK399539 | MK399446 | MK399642 | MK399670 |
276 | Anisolabididae | MK399547 | MK399451 | MK399645 | MK399673 | |
C_fletch | Challia fletcheri | Pygidicranidae | NC_018538 | - | - | |
L_ripar | Labidura riparia | Labiduridae | KX069089 | - | AB119553 | KX069030 |
N_tenui | Nala tenuicornis | Labiduridae | KX069090 | - | - | KX069055 |
N_livid | Nala lividipes | Labiduridae | KX069069 | - | - | KX069048 |
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Stuart, O.P.; Binns, M.; Umina, P.A.; Holloway, J.; Severtson, D.; Nash, M.; Heddle, T.; van Helden, M.; Hoffmann, A.A. Morphological and Molecular Analysis of Australian Earwigs (Dermaptera) Points to Unique Species and Regional Endemism in the Anisolabididae Family. Insects 2019, 10, 72. https://doi.org/10.3390/insects10030072
Stuart OP, Binns M, Umina PA, Holloway J, Severtson D, Nash M, Heddle T, van Helden M, Hoffmann AA. Morphological and Molecular Analysis of Australian Earwigs (Dermaptera) Points to Unique Species and Regional Endemism in the Anisolabididae Family. Insects. 2019; 10(3):72. https://doi.org/10.3390/insects10030072
Chicago/Turabian StyleStuart, Oliver P., Matthew Binns, Paul A. Umina, Joanne Holloway, Dustin Severtson, Michael Nash, Thomas Heddle, Maarten van Helden, and Ary A. Hoffmann. 2019. "Morphological and Molecular Analysis of Australian Earwigs (Dermaptera) Points to Unique Species and Regional Endemism in the Anisolabididae Family" Insects 10, no. 3: 72. https://doi.org/10.3390/insects10030072